Endophytic Fungi for Crops Adaptation to Abiotic Stresses

Microorganisms, Journal Year: 2024, Volume and Issue: 12(7), P. 1357 - 1357

Published: July 2, 2024

Endophytic fungi (EFs) have emerged as promising modulators of plant growth and stress tolerance in agricultural ecosystems. This review synthesizes the current knowledge on role EFs enhancing adaptation crops to abiotic stress. Abiotic stresses, such drought, salinity, extreme temperatures, pose significant challenges crop productivity worldwide. shown remarkable potential alleviating adverse effects these stresses. Through various mechanisms, including synthesis osmolytes, production stress-related enzymes, induction defense enhance resilience stressors. Moreover, promote nutrient uptake modulate hormonal balance plants, further plants. Recent advancements molecular techniques facilitated identification characterization stress-tolerant EF strains, paving way for their utilization practices. Furthermore, symbiotic relationship between plants offers ecological benefits, improved soil health a reduced dependence chemical inputs. However, remain understanding complex interactions host well scaling up application diverse systems. Future research should focus elucidating mechanisms underlying endophytic-fungal-mediated developing sustainable strategies harnessing production.

Language: Английский

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Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 25, 2024

Abstract Endophytic fungi are microorganisms that considered as a potential source of natural compounds, and can be applied in various industries. The aims this research were molecular identification endophytic isolated from the Gundelia tournefortii stems, investigation their biological activities well phenolic fatty acid profile. Surface sterilized stems G. placed on potato dextrose agar (PDA) to isolate fungal endophytes. Genomic DNA was extracted by CTAB method, PCR amplification performed ITS 1 4 primers. enzyme production determined based formation clear zone appeared around colonies fungus. anti-oxidant activity evaluated measuring amount free radicals DPPH. Also, total phenol flavonoid contents measured obtained Folin-Ciocalteu aluminum chloride colorimetric methods, respectively. Moreover, separation acids done HPLC GC, Phylogenetic analysis Internal Transcribed Spacer (ITS) region, five isolates identified following: Aspergillus niger , Penicillium glabrum Alternaria alternata A. tenuissima Mucor circinelloides . Evaluation enzymatic properties showed P. gabrum (31 ± 1.9 mm), (23 1.7) had more ability for producing pectinase cellulase. extract (IC 50 = 471 29 µg/mL) highest properties, followed 512 19 µg/mL). greatest phenols flavonoids (8.2 0.4 mg GAL/g 2.3 0.3 QE/g, respectively). quantification rosmarinic acid, para-coumaric meta-coumaric (42.02 1.31, 7.53 0.19, 5.41 0.21 mg/g, respectively) main studied fungi. confirmed that, all fungi, stearic (27.9–35.2%), oleic (11.3–17.3%), palmitic (16.9–23.2%), linoleic (5.8–11.6%), caprylic (6.3–10.9%). Our finding bioactive which could used This is first report associated with provides knowledge future use biotechnological processes.

Language: Английский

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Therapeutic Potential of Endophytic Microbes: Emphasizing Both Fungal and Bacterial Endophytes

Applied Microbiology, Journal Year: 2025, Volume and Issue: 5(1), P. 5 - 5

Published: Jan. 5, 2025

This review explores the diverse applications and therapeutic potential of endophytic microbes, emphasizing both fungal bacterial endophytes. These microorganisms reside within plant tissues without causing harm play an important role in enhancing growth, nutrient acquisition, resistance to pathogens. They produce phytohormones, facilitate uptake, solubilize essential nutrients, fix nitrogen, improve stress tolerance. Furthermore, endophytes contribute agricultural sustainability by producing growth regulators, providing biocontrol against pathogens through antimicrobial compounds, competing for resources. Integrating microbes into practices can reduce reliance on chemical fertilizers pesticides, promoting eco-friendly sustainable farming. highlights dual fostering agriculture novel applications. By minimizing dependence inputs, support environmental health while boosting crop yields. The synthesis underscores importance leveraging tackle global food security challenges.

Language: Английский

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Exploring the potential of saponins from Dicotyledonous plants in sustainable pest management: innovations and challenges; A reveiw

Journal of Natural Pesticide Research, Journal Year: 2025, Volume and Issue: unknown, P. 100111 - 100111

Published: Jan. 1, 2025

Language: Английский

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Biological Guardians: Unveiling Microbial Solutions to Combat Cannabis sativa Fungal Pathogens

Stresses, Journal Year: 2025, Volume and Issue: 5(1), P. 16 - 16

Published: Feb. 17, 2025

Cannabis (Cannabis sativa L.) is one of the earliest cultivated crops and valued for its medicinal compounds, food, fibre, bioactive secondary metabolites. The rapid expansion cannabis industry has surpassed development production system knowledge. scientific community currently focuses on optimising agronomic environmental factors to enhance yield quality. However, cultivators face significant challenges from severe pathogens, with limited effective control options. principal diseases include root rot, wilt, bud powdery mildew, stunt disease, microorganisms that reduce post-harvest Sustainable management strategies involve utilising clean planting stocks, modifying conditions, implementing sanitation, applying fungal bacterial biological agents, drawing decades research other crops. Plant–microbe interactions can promote growth regulate metabolite production. This review examines recent literature pathogen in indoor using biocontrol agents. Specific morphological, biochemical, characteristics hinder implementation cannabis. Subsequent investigations should focus elucidating plant–microbe essential effectiveness methodologies cultivation systems.

Language: Английский

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Isolation and Functional Characterization of Endophytic Bacteria from Muscadine Grape Berries: A Microbial Treasure Trove

Cells, Journal Year: 2025, Volume and Issue: 14(5), P. 369 - 369

Published: March 3, 2025

Muscadine grapes are renowned for their unique traits, natural disease resistance, and rich bioactive compounds. Despite extensive research on phytochemical properties, microbial communities, particularly endophytic bacteria, remain largely unexplored. These bacteria play crucial roles in plant health, stress tolerance, ecological interactions. This study represents the first comprehensive effort to isolate, identify, functionally characterize bacterial endophytes inhabiting muscadine grape berries using a culture-dependent approach. We isolated diverse species spanning six genera-Bacillus, Staphylococcus, Paenibacillus, Calidifontibacillus, Curtobacterium, Tatumella. Microscopic physiological analysis revealed variations morphology, with isolates demonstrating adaptability varied temperatures. Cluster-based indicated functional specialization among isolates, from Pseudomonadota Actinomycetota exhibiting superior growth-promoting abilities, whereas Bacillota displayed potential biocontrol probiotic properties. Among them, Tatumella ptyseos demonstrated exceptional including indole-3-acetic acid production, nitrogen fixation, phosphate solubilization, carbohydrate fermentation. Additionally, Bacillus spp. showed presumptive potential, while Paenibacillus cineris emerged as candidate. The identification of Calidifontibacillus erzurumensis novel further expands known biodiversity grape-associated microbes. findings provide insights into metabolic diversity endophytes, highlighting agricultural biotechnological applications.

Language: Английский

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Endophytic Fungi for Crops Adaptation to Abiotic Stresses

Published: Feb. 22, 2024

Endophytic fungi (EF) have emerged as promising modulators of plant growth and stress tolerance in agricultural ecosystems. This review synthesizes current knowledge on the role EF en-hancing crop adaptation to abiotic stress. Abiotic stresses, such drought, salinity, extreme temperatures, pose significant challenges productivity worldwide. shown re-markable potential alleviating adverse effects these stresses. Through various mechanisms including synthesis osmolytes, production stress-related enzymes, induction de-fense mechanisms, enhance resilience stressors. Moreover, promote nutrient uptake modulate hormonal balance plants, further enhancing tolerance. Recent ad-vancements molecular techniques facilitated identification characterization stress-tolerant endophytic fungal strains, paving way for their utilization prac-tices. Furthermore, symbiotic relationship between plants offers ecological benefits, improved soil health reduced dependence chemical inputs. However, remain understanding complex interactions host well scaling up application diverse systems. Future research should focus elucidating underlying fungal-mediated developing sustainable strategies harnessing production.

Language: Английский

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In vitro and In silico investigation deciphering novel antifungal activity of endophyte Bacillus velezensis CBMB205 against Fusarium oxysporum

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 3, 2025

Endophytes from medicinal plants are potential biocontrol agents against Fusarium oxysporum f. sp. cubense (Foc), which is the causative fungus of banana wilt disease. In present study, endophytic bacterium was isolated Globba racemosa and their antagonistic activities Foc were studied, probable molecular mechanism antagonism predicted by docking studies. The 16SrRNA sequencing confirmed isolate to be Bacillus velezensis CBMB205 (EG2). isolates distortion fungal hyphae illustrated in SEM. metabolites identified FTIR, suggesting presence C-H, CH3 O-H groups. Two major such as β-amyrin dihydroxy octadecenoic acid (DA) LC-MS analysis. Molecular studies suggested that these showed binding with chitin synthase 1 1,3-glucan pathogenic fungi. energy (BE) interaction between synthase-1 (CS-1), (1,3-GS) estimated -10.17 kcal/mol − 9.5 kcal/mol, respectively. BE CS-1 1,3-GS determined -2.43 3.4 current study demonstrated EG2 towards provided a silico also provides insight into developing metabolite-based antifungal for various agricultural applications.

Language: Английский

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Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes

Antibiotics, Journal Year: 2025, Volume and Issue: 14(1), P. 108 - 108

Published: Jan. 20, 2025

Background: The increasing prevalence of drug-resistant tuberculosis (TB) underscores the urgent need for novel antimicrobial agents. Methods: This study integrates cultivation optimization, nuclear magnetic resonance (NMR) fingerprinting, and principal component analysis (PCA) to explore microbial secondary metabolites as potential anti-TB Results: Using combined approach, 11 bioactive compounds were isolated identified, all exhibiting anti-Mycobacterium bovis BCG activity. Notable findings include borrelidin, a potent threonyl-tRNA synthetase inhibitor with broad biological activities, L-O-Lac-L-Val-D-O-Hiv-D-Val, peptide first time from plant endophyte, demonstrating broad-spectrum Additionally, elaiophylin polycyclic tetramate macrolactams (PTMs) displayed significant bactericidal effects, achieving complete inhibition at 72 h PTMs marking their reported also identified bafilomycins scaffolds drug development, showcasing rapid activity low MIC values. Conclusions: These emphasize value reservoir provide new avenues developing next-generation therapies.

Language: Английский

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Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A review

Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100455 - 100455

Published: Feb. 1, 2025

Language: Английский

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