Bio-Based Surfactants and Biosurfactants: An Overview and Main Characteristics

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 863 - 863

Published: Feb. 13, 2025

Natural surfactants are surface-active molecules synthesized from renewable resources (i.e., plants, animals, or microorganisms) and possess properties comparable to conventional surfactants, making them an environmentally friendly potential alternative petrochemical surfactants. Additionally, they exhibit biological such as anti-microbial properties, biodegradability, less toxicity, allowing their use in everyday products with minimal risk human health the environment. Based on mode of production, natural can be classified into first-generation bio-based second-generation biosurfactants, although definition may vary depending author literature. This review offers extensive classification focusing composition, sources, production methods, applications across various industries. Furthermore, main challenges future perspectives discussed.

Language: Английский

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Antimicrobial Activity of Bacillus Cyclic Lipopeptides and Their Role in the Host Adaptive Response to Changes in Environmental Conditions

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 336 - 336

Published: Jan. 2, 2025

Bacillus cyclic lipopeptides (CLP), part of the three main families—surfactins, iturins, and fengycins—are secondary metabolites with a unique chemical structure that includes both peptide lipid components. Being amphiphilic compounds, CLPs exhibit antimicrobial activity in vitro, damaging membranes microorganisms. However, concentrations used vitro are difficult to achieve natural conditions. Therefore, environment, alternative mechanisms action by more likely, such as inducing apoptosis fungal cells, preventing microbial adhesion substrate, promoting death phytopathogens stimulating plant immune responses. In addition, low act signaling molecules Bacillus’s own metabolism, when environmental conditions change, they form an adaptive response host bacterium. Namely, trigger differentiation bacterial population into various specialized cell types: competent flagellated matrix producers, spores. this review, we have summarized current understanding under experimental We also shown relationship between some regulatory pathways involved CLP biosynthesis differentiation, well role determine changes physiological state subpopulations shifts

Language: Английский

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Molecular structural arrangement in quorum sensing and bacterial metabolic production

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(2)

Published: Feb. 1, 2025

Language: Английский

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An in-depth study of the growth inhibition of Vibrio parahaemolyticus by Surfactin and its effects on cell membranes, ROS levels and gene transcription

Journal of Invertebrate Pathology, Journal Year: 2025, Volume and Issue: 211, P. 108298 - 108298

Published: March 3, 2025

Language: Английский

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The Metabolic and Physiological Responses to Spaceflight of a Lipopeptide‐Producing Bacillus subtilis

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(3)

Published: March 1, 2025

ABSTRACT Outer space is an extreme environment and the survival of many microorganisms after spaceflight well established. However, adaptations Bacillus subtilis to stress, particularly metabolism, are largely unknown. Here, we first performed a mission B. TD7 strain compared spaceflight‐exposed with wild‐type in terms their phenotype, biofilm formation secondary metabolism. The exhibited slower growth, different morphology decreased formation. Importantly, decline lipopeptide production was observed spaceflight. Multi‐omics approaches were used uncover molecular mechanisms underlying metabolism 997 differentially expressed genes (DEGs) found, involving TCA cycle, fatty acid degradation, amino biosynthesis quorum sensing systems. Further analysis 26 lipopeptide‐related DEGs further elucidated relationship between regulation. Our findings could contribute better understanding microbial adaptation mechanisms.

Language: Английский

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Genomic and metabolomic insights into the antimicrobial compounds and plant growth-promoting potential of Bacillus velezensis B115

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 28, 2025

The B115 strain, isolated from the inter-root soil of healthy plants in a continuous cropping site Panax notoginseng, was identified as Bacillus velezensis by 16S rDNA sequence comparison and comparative genomic analysis. is strain beneficial microorganisms present zone plants, with favorable plant growth-promoting properties antagonistic effects against pathogen Fusarium oxysporum. However, whole genome remains unclear, thus restricting its potential applications. To address this gap, has been sequenced annotated to elucidate molecular mechanisms underlying antimicrobial activities. analysis revealed that comprises single circular chromosome 4,200,774 bp plasmid region 16,878 long, possessing GC content 45.95%. Moreover, 4349 protein-coding genes were predicted. Notably, contains substantial number (103) involved biosynthesis, transport, catabolism secondary metabolites. Through mining, 13 BGCs 540 encoding metabolites predicted roles identified, including members surfactin fengycin families. Utilizing LC–MS/MS technologies, 2318 detected fermentation broth B. B115, encompassing compounds such Corynebactin, Gamabufotalin, Pracinostat, Indoleacetic acid, (8)-Gingerol, Luteolin, Liquiritigenin, other antimicrobial, growth-promoting, antioxidant, antitumor properties. By exploring metabolite-related predicting based on whole-genome results, we further basis for ability promote growth inhibit activity.

Language: Английский

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Simultaneously Enhancing the Catalytic Activity and Thermostability of Pseudomonas aeruginosa Aminopeptidase via Structure-based Design

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Aminopeptidases are crucial hydrolases in the food and pharmaceutical industries. This study addresses need to enhance catalytic performance of Pseudomonas aeruginosa aminopeptidase (PaAps) through a multifaceted computational design strategy. We introduced single-site mutations followed by combinatorial develop mutant library, identifying optimal S112D, which demonstrated 5.19-fold increase activity nearly doubled thermostability compared wild type. The kinetic parameters (kcat, kcat/Km, Vmax) S112D were found be 4.36, 6.52, 4.36 times greater than those type, respectively. Molecular dynamics (MD) simulations revealed that induced global conformational changes, resulting more open active pocket facilitated better binding with substrate, thereby improving stability. Additionally, exhibited closer nucleophilic attack distance stronger hydrogen bonding interactions, further boosting efficiency. Remarkably, as well showed hydrolytic on both corn soybean proteins. hydrolysis rate protein was approximately 1.92 PaAps, for protein, it is roughly 1.84 times. These findings offered valuable insights developing efficient enzyme modification strategies.

Language: Английский

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The TerC family metal chaperone MeeY enables surfactin export in Bacillus subtilis

Journal of Bacteriology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

ABSTRACT TerC family proteins are widely conserved integral membrane with functions related to metal transport. In Bacillus subtilis , the MeeF and MeeY play overlapping roles in metalation of manganese-requiring extracellular enzymes. interact secretion translocon SecYEG metalate either during or after protein translocation. Here, we demonstrate that swarming motility is dependent on MeeY. This defect can be complemented extracellularly correlated a loss surfactin. Surfactin export mediated by SwrC, an RND efflux pump previously shown co-immunoprecipitation studies. The amendment growth medium manganese has long been known enhance surfactin production. We suggest model which enhanced MeeY-dependent lipopeptide export. IMPORTANCE produces surfactin, powerful detergent-like compound intercellular communication, surface motility, as broad-spectrum antimicrobial agent. Production cyclic lipopeptide, depends non-ribosomal peptide synthase followed member resistance-nodulation-cell division (RND) proteins. production additionally requires MeeY, exports ions support function secreted metalloenzymes. propose interacts SwrC facilitate binding from cell. These results may explain long-appreciated role divalent industrial fermentation.

Language: Английский

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Distantly related bacteria share a rigid proteome allocation strategy with flexible enzyme kinetics

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(18)

Published: April 29, 2025

Bacteria are known to allocate their proteomes according how fast they grow, and the allocation strategies employed strongly affect bacterial adaptation different environments. Much of what is currently about proteome based on extensive studies model organism Escherichia coli . It not clear much E. ’s strategy applicable other species, particularly since species can grow at vastly rates even in same growth condition. In this study, we investigate differences nutrient-dependent programs adopted by several distantly related including Vibrio natriegens , one fastest-growing bacteria known. Extensive quantitative characterization across conditions reveals an invariant program response changing nutrients despite systemic, species-specific enzyme kinetics. This organized rate but a common internal metric nutrient quality after scaling away kinetics, with faster behaving as if it growing under higher temperature. The flexibility kinetics rigidity defy notions evolvability resource optimization. Our results suggest existence blueprint shared diverse implications underlying regulatory strategies. Further knowledge organization such phylogeny-transcending relations also promises simplify bottom–up description understanding behaviors ecological communities.

Language: Английский

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Unravelling the Molecular Dialogue of Beneficial Microbe−Plant Interactions

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

ABSTRACT Plants are an intrinsic part of the soil community, which is comprised a diverse range organisms that interact in rhizosphere through continuous molecular communications. The dialogue within plant microbiome involves complex repertoire primary and secondary metabolites different liquid matrices biofilms. Communication functions likely to involve membrane‐less organelles formed by liquid−liquid phase separation proteins natural deep eutectic solvents play role as alternative media water. We discuss chemistry inter‐organism communication signalling biosphere allows plants discriminate between harmful, benign beneficial microorganisms. summarize current information concerning chemical underpins plant−microbe host‐range specificity. highlight how regulated production, perception processing reactive oxygen species (ROS) used microbes communities shape microbiome.

Language: Английский

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