Biotransformation of quercetin by Bacillus subtilis and anticancer activity evaluation: in vitro and in Silico

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 2, 2025

Language: Английский

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De novo biosynthesis of quercetin in Yarrowia Lipolytica through systematic metabolic engineering for enhanced yield

Bioresources and Bioprocessing, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 22, 2025

Abstract Kaempferol and quercetin possess various biological activities, making them valuable in food medicine. However, their production via traditional methods is often inefficient. This study aims to address this gap by engineering the yeast Yarrowia lipolytica achieve high yields of these flavonoids. We designed a kaempferol biosynthetic pathway integrating multiple-copy fusion enzyme expression modules, F3H-(GGGGS) 2 -FLS, into genome with an optimized linker (GGGGS) enhance from naringenin. To synthesize de novo, we introduced FMOCPR gene kaempferol-synthesizing strain using p FBAin promoter. Notably, increasing glucose concentration effectively boosted both Our results demonstrated titers reaching 194.30 ± 7.69 278.92 11.58 mg/L, respectively, shake-flask cultures. These findings suggest that Y. promising platform for efficient flavonoid-derived products.

Language: Английский

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Systematic Engineering of Eriodictyol-7-O-glucoside Biosynthetic Pathway through Enzyme Screening and Gene Regulation

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

The natural product eriodictyol-7-O-glucoside is more water-soluble and stable than eriodictyol also exhibits novel biological activities functions. However, its biosynthesis hindered by the limited catalytic efficiency of key enzymes. Furthermore, intricate interplay between cell growth UDP-glucose supply adds another layer complexity, rendering engineering productive strains challenging. To address this challenge, we introduced a "top-down" strategy in which bioinformatics analysis was initially used to identify six specific flavonoid 7-O-glucosyltransferases uniquely capable converting into 7-O-glucoside derivative. By manipulation plasmid copy number promoter strength, expression levels most promising from Arabidopsis thaliana were precisely fine-tuned, resulting remarkable 66.16% increase production. improve enhancement process, focused on overexpression two pivotal enzymes: phosphoglucose mutase (encoded pgm gene) pyrophosphorylase galU gene). This strategic step implemented significantly availability glycosylation donors. Moreover, concentration increased ensure ample feedstock. Finally, through comprehensive optimization fermentation conditions, production substantially enhanced, with staggering 1187.36 mg L-1 obtained (molar conversion rate: 94.99%) at substrate titer 800 after 48 h fermentation. best engineered strain produced 2.70 g 5 L bioreactor. study provides an effective sustainable method for high-yield glycoside derivatives.

Language: Английский

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Regulation of the phenolic release and conversion in oats (Avena sativa L.) by co-microbiological fermentation with Monascus anka, Saccharomyces cerevisiae and Bacillus subtilis

Bioprocess and Biosystems Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Language: Английский

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