Metabolic Engineering of Escherichia coli for Efficient l-Isoleucine Production based on the Citramalate Pathway DOI

Qiquan Zhang,

Yuheng Wang, Xiaolu Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

l-isoleucine, a value-added branched-chain amino acid, has been widely used in the food, feed, and pharmaceutical industries. However, production efficiency of l-isoleucine is relatively low due to complex inefficient biosynthetic network canonical threonine pathway. Here, we report exploitation concise citramalate pathway for development an efficient producer. First, chassis strain key genes were screened establishment Subsequently, importer was identified applied enhance citramalate's utilization efficiency. Finally, plasmid-free high-l-isoleucine producer developed by enhancement efflux, optimization rate-limiting gene expression, introduction nonoxidative glycolysis Fed-batch fermentation final 10-L bioreactor produced 56.6 g/L with productivity 1.66 g/L/h, which highest titer reported. This study paves way construction microbial cell factories related derivatives based on

Language: Английский

Metabolic Engineering of Escherichia coli for Efficient l-Isoleucine Production based on the Citramalate Pathway DOI

Qiquan Zhang,

Yuheng Wang, Xiaolu Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

l-isoleucine, a value-added branched-chain amino acid, has been widely used in the food, feed, and pharmaceutical industries. However, production efficiency of l-isoleucine is relatively low due to complex inefficient biosynthetic network canonical threonine pathway. Here, we report exploitation concise citramalate pathway for development an efficient producer. First, chassis strain key genes were screened establishment Subsequently, importer was identified applied enhance citramalate's utilization efficiency. Finally, plasmid-free high-l-isoleucine producer developed by enhancement efflux, optimization rate-limiting gene expression, introduction nonoxidative glycolysis Fed-batch fermentation final 10-L bioreactor produced 56.6 g/L with productivity 1.66 g/L/h, which highest titer reported. This study paves way construction microbial cell factories related derivatives based on

Language: Английский

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