Intensification of 2′-Fucosyllactose biosynthesis pathway by using a novel fucosyltransferase from Bacillus cereus DOI Creative Commons

Kainuo Zhang,

Miaomiao Gao, Chang Cao

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 30, 2025

2'-Fucosyllactose (2'-FL) is an oligosaccharide that can be synthesized in the human body and known for its health-promoting prebiotic effects. The biosynthesis of 2'-FL using microorganisms has received attention recently due to increased application nutritional medical infant formulations. This work attempts new Bacillus cereus α-1,2-fucosyltransferase (FutCB) de novo synthesis Escherichia coli (E. coli). Additionally, knocking out LacZ WaaF genes alongside overexpression key gmd, manB, wcaG, manC enhances availability necessary precursors GDP-L-fucose lactose 2'-FL. use constitutive promoters achieved better control over production during fed-batch fermentation. After 64 h fermentation, modified E. strains produced 121.4 g/L with a yield 1.90 g/L/h, resulting impressive output. These results together indicate potential large-scale, high-yield form basis much more refinement done. next step will focus on maximum substrate utilization, alteration gene regulation, improvement commercial-scale synthesis.

Language: Английский

Intensification of 2′-Fucosyllactose biosynthesis pathway by using a novel fucosyltransferase from Bacillus cereus DOI Creative Commons

Kainuo Zhang,

Miaomiao Gao, Chang Cao

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 30, 2025

2'-Fucosyllactose (2'-FL) is an oligosaccharide that can be synthesized in the human body and known for its health-promoting prebiotic effects. The biosynthesis of 2'-FL using microorganisms has received attention recently due to increased application nutritional medical infant formulations. This work attempts new Bacillus cereus α-1,2-fucosyltransferase (FutCB) de novo synthesis Escherichia coli (E. coli). Additionally, knocking out LacZ WaaF genes alongside overexpression key gmd, manB, wcaG, manC enhances availability necessary precursors GDP-L-fucose lactose 2'-FL. use constitutive promoters achieved better control over production during fed-batch fermentation. After 64 h fermentation, modified E. strains produced 121.4 g/L with a yield 1.90 g/L/h, resulting impressive output. These results together indicate potential large-scale, high-yield form basis much more refinement done. next step will focus on maximum substrate utilization, alteration gene regulation, improvement commercial-scale synthesis.

Language: Английский

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