Optimization and characterization of polyhydroxybutyrate produced by Halomonas meridiana using orange peel waste DOI Creative Commons

Mahmoud H. Hendy,

Amr M. Shehabeldine, Amr H. Hashem

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 19, 2025

Abstract The production of bioplastics from marine microorganisms is significantly relevant in the field biotechnological applications for sustainable ecological management. Nevertheless, expense associated with PHB substantial and regarded as primary obstacle to its industrialization. In this study, orange peel waste served a carbon source enhance efficiency. Among 15 strains evaluated, MH 96 was selected due high salt tolerance efficient utilization substrate. highest producing strain MH96 genetically identified using 16S rRNA sequencing Halomonas meridiana submitted GenBank under accession numbers PP826284. optimal fermentation conditions were evaluated through single-factor optimization. Upon completion response surface optimization, Plackett–Burman Box-Behnken design experiments conducted utilizing outcomes final parameters inoculum size 1.74, (NH 4 ) 2 HPO concentration 1.0 pH 6.37, yield 5.94 g/L. characterization extracted biopolymer by NMR, FTIR, XRD, thermal properties used examine PHB, gas chromatography-mass spectrometry (GC–MS) proves presence 2-butenoic acid, 1-methyl ethyl ester, tetradecane, hexadecanoic methyl docosanoic 8,9,13-trihydroxy-. Methyl which confirmed structure polymer PHB.

Language: Английский

Analyzing and optimizing the lipids production potential of the oleaginous yeast Candida neerlandica from synthetic carbon sources and real wastes DOI

Gayathri Vemparala,

Abhishiktha Chiliveru,

Sameena Begum

et al.

Next research., Journal Year: 2025, Volume and Issue: unknown, P. 100196 - 100196

Published: Feb. 1, 2025

Language: Английский

Citations

0
Optimization and characterization of polyhydroxybutyrate produced by Halomonas meridiana using orange peel waste DOI Creative Commons

Mahmoud H. Hendy,

Amr M. Shehabeldine, Amr H. Hashem

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 19, 2025

Abstract The production of bioplastics from marine microorganisms is significantly relevant in the field biotechnological applications for sustainable ecological management. Nevertheless, expense associated with PHB substantial and regarded as primary obstacle to its industrialization. In this study, orange peel waste served a carbon source enhance efficiency. Among 15 strains evaluated, MH 96 was selected due high salt tolerance efficient utilization substrate. highest producing strain MH96 genetically identified using 16S rRNA sequencing Halomonas meridiana submitted GenBank under accession numbers PP826284. optimal fermentation conditions were evaluated through single-factor optimization. Upon completion response surface optimization, Plackett–Burman Box-Behnken design experiments conducted utilizing outcomes final parameters inoculum size 1.74, (NH 4 ) 2 HPO concentration 1.0 pH 6.37, yield 5.94 g/L. characterization extracted biopolymer by NMR, FTIR, XRD, thermal properties used examine PHB, gas chromatography-mass spectrometry (GC–MS) proves presence 2-butenoic acid, 1-methyl ethyl ester, tetradecane, hexadecanoic methyl docosanoic 8,9,13-trihydroxy-. Methyl which confirmed structure polymer PHB.

Language: Английский

Citations

0