BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 17, 2025
Enhanced glycolytic levels in cancer cells are a common characteristic of many types. Modulation metabolism is crucial for enhancing the efficacy therapy. The specific role 6-methoxyflavone regulating remains unclear. This study aimed to elucidate impact on cervical and its clinical relevance. tandem mass tag (TMT) proteomic analysis was used identify significantly enriched biological processes pathways HeLa after treatment with 6-methoxyflavone. Additionally, differential expression glycolysis-related proteins validated using parallel reaction monitoring (PRM) proteomics. Untargeted targeted metabolomics analyses were differentially expressed metabolites. Furthermore, alternative splicing, new transcripts, domain detect effects structures genes proteins. Subcellular localization, molecular docking, non-covalent interaction subcellular affinity proteins, sites interactions. Clinical characteristics immunological correlation relationships between clinicopathological characteristics, survival, prognosis, immune-related indicators patients cancer. Finally, glycolysis stress tests enzyme activity assays verify effect cells. TMT PRM proteomics, as well untargeted results, showed that downregulated metabolites cells, functions cytoplasm underwent changes. 6-Methoxyflavone had good nine all which sites. immune five survival four After 6-methoxyflavone, basal level, maximum capacity, reserve downregulated. inhibited pyruvate kinase. energy through pathway. may be related tumor microenvironment, checkpoints, immunotherapy
Language: Английский