Dental pulp stem cells – A basic research and future application in regenerative medicine

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 178, P. 116990 - 116990

Published: July 17, 2024

Dental pulp is a valuable and accessible source of stem cells (DPSCs) with characteristics similar to mesenchymal cells. DPSCs can regenerate range tissues their potential for clinical application in regenerative medicine promising. have been found express low levels Class II HLA-DR (MHC) molecules, making them candidates allogeneic transplantation without matching the donor's tissue. Research on correlation between non-coding RNAs (ncRNAs) human dental provides promising insights into use these settings wide medical conditions. It possible number ncRNAs order restore functional role downregulated that are correlated osteoblastogenesis, or suppress overexpressed associated osteoclast differentiation some cases.

Language: Английский

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Decoding the regulatory landscape of lncRNAs as potential diagnostic and prognostic biomarkers for gastric and colorectal cancers

Clinical and Experimental Medicine, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 31, 2024

Colorectal cancer (CRC) and gastric (GC) are major contributors to cancer-related mortality worldwide. Despite advancements in understanding molecular mechanisms improved drug treatments, the overall survival rate for patients remains unsatisfactory. Metastasis resistance challenges contributing high both CRC GC. Recent research has shed light on role of long noncoding RNAs (lncRNAs) development progression these cancers. LncRNAs regulate gene expression through various mechanisms, including epigenetic modifications interactions with microRNAs (miRNAs) proteins. They can serve as miRNA precursors or pseudogenes, modulating at transcriptional post-transcriptional levels. Additionally, circulating lncRNAs have emerged non-invasive biomarkers diagnosis, prognosis, prediction therapy response This review explores intricate relationship between CRC/GC, encompassing their roles development, progression, chemoresistance. Furthermore, it discusses potential therapeutic targets malignancies. The interplay lncRNAs, miRNAs, tumor microenvironment is also highlighted, emphasizing impact complexity biology. Understanding regulatory landscape governed by GC crucial effective diagnostic prognostic biomarkers, well novel strategies. provides a comprehensive overview current knowledge paves way further exploration key players management

Language: Английский

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Potential roles of lncRNA-XIST/miRNAs/mRNAs in human cancer cells

Clinical & Translational Oncology, Journal Year: 2023, Volume and Issue: 25(7), P. 2015 - 2042

Published: Feb. 28, 2023

Language: Английский

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Single-Cell Transcriptomic Approaches for Decoding Non-Coding RNA Mechanisms in Colorectal Cancer

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(2), P. 24 - 24

Published: March 10, 2025

Non-coding RNAs (ncRNAs) play crucial roles in colorectal cancer (CRC) development and progression. Recent developments single-cell transcriptome profiling methods have revealed surprising levels of expression variability among seemingly homogeneous cells, suggesting the existence many more cell types than previously estimated. This review synthesizes recent advances ncRNA research CRC, emphasizing bioinformatics approaches for their analysis. We explore computational tools used identification, characterization, functional prediction with a focus on RNA sequencing (scRNA-seq) data. The highlights key strategies, including sequence-based structure-based approaches, machine learning applications, multi-omics data integration. discuss how these techniques can be applied to analyze differential expression, perform enrichment, construct regulatory networks involving ncRNAs CRC. Additionally, we examine role leveraging as diagnostic prognostic biomarkers also scRNA-seq studies revealing heterogeneity aims provide comprehensive overview current state CRC outline future directions this rapidly evolving field, integration experimental validation advance our understanding biology

Language: Английский

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Multidimensional transcriptomics based to illuminate the mechanisms of taurine metabolism in immune resistance of pancreatic cancer

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 31, 2025

Pancreatic cancer, a highly malignant tumor of the digestive system, is characterized by microenvironment with high degree immunosuppression. This immunosuppressive property poses significant challenges, as it hampers effective infiltration immune cells and impairs their ability to exert cytotoxic effects. The metabolic process taurine has emerged crucial factor in modulating functions activities cells. Intervening metabolism holds potential reshape microenvironment, thereby enhancing recognize eliminate To explore therapeutic relationship between disorders pancreatic cancer immunotherapy, we employed multiple software packages, including "Seurat", "DoubletFinder", "Harmony", "GSVA", "CellChat" analyze single-cell data spatial transcriptomic cancer. In present study, four distinct cell subsets, namely RPS4Y1+ cells, LYZ+ CPE+ MKI67+ were identified for first time. CNV score highlighted role within Through cell-communication analysis, crosstalk among fibroblasts, CD8+ T was identified, offering novel insights into immunotherapy strategies, which strengthened co-localization analysis transcriptomics. Furthermore, conducting combined survival data, LY6D target. co-culture experiments uncovered underlying mechanism regulating imbalance establishment "taurine-immune crosstalk" criteria this study effectively paves way immunotherapy. conclusion, current research underscores significance Targeting may represent approach reversing "stiff-cancer" characteristics

Language: Английский

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Long non-coding RNA MAFG-AS1: A promising therapeutic target for human cancers

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 163, P. 114756 - 114756

Published: April 25, 2023

Long non-coding RNAs (lncRNAs) are commonly known for their important characters in cancer progression. LncRNA MAFG-antisense 1 (AS1) (MAFG-AS1) has been discovered as a novel oncogenic lncRNA several years. Accumulating data have demonstrated abnormal overexpression of MAFG-AS1 various human cancers, including breast, bladder, liver, gastric, and lung. Importantly, through regulating microRNAs cell signaling pathways, to exhibit biological effects, proliferation, metastasis, epithelial-mesenchymal transition (EMT). Meanwhile, is closely linked with histological grade, TNM stage, extensive depth invasion, poor OS, lymph node metastasis (LNM). In the present review, authors summarized previous studies on properties, molecular mechanisms, clinicopathological cancers. summary, promising prognostic marker potential therapeutic target treatment.

Language: Английский

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Long Non-Coding RNAs in Venous Thromboembolism: Where Do We Stand?

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(15), P. 12103 - 12103

Published: July 28, 2023

Venous thromboembolism (VTE), a common condition in Western countries, is cardiovascular disorder that arises due to haemostatic irregularities, which lead thrombus generation inside veins. Even with successful treatment, the resulting disease spectrum of complications considerably affects patient’s quality life, potentially leading death. Cumulative data indicate long non-coding RNAs (lncRNAs) may have role VTE pathogenesis. However, clinical usefulness these as biomarkers and potential therapeutic targets for management yet unclear. Thus, this article reviewed emerging evidence on lncRNAs associated activity coagulation system, has central Until now, ten been implicated pathogenesis, among MALAT1 one more evidence. Meanwhile, five reported affect expression TFPI2, an important anticoagulant protein, but none described development. More investigation field needed help dissect pathways, aiding prediction, prevention treatment.

Language: Английский

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lncRNA TUG1 as potential novel biomarker for prognosis of cardiovascular diseases

Epigenomics, Journal Year: 2023, Volume and Issue: 15(23), P. 1273 - 1290

Published: Dec. 1, 2023

Globally, cardiovascular diseases (CVDs) are among the leading causes of death. In light high prevalence and mortality CVDs, it is imperative to understand molecules involved in CVD pathogenesis signaling pathways that they initiate. This may facilitate development more precise expedient diagnostic techniques, identification effective prognostic potential therapeutic targets. Numerous studies have examined role lncRNAs, such as TUG1, recent years. According this review article, TUG1 can be considered a biomarker for predicting prognosis CVD.

Language: Английский

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YY1-induced LncRNA-TUG1 elevates YOD1 to promote cell proliferation and inhibit bortezomib sensitivity in multiple myeloma

Leukemia & lymphoma/Leukemia and lymphoma, Journal Year: 2023, Volume and Issue: 64(6), P. 1161 - 1174

Published: April 20, 2023

Taurine upregulated gene 1 (TUG1) has been implicated in the onset and progression of various malignancies. The current study aimed to evaluate biological function potential mechanisms TUG1 multiple myeloma (MM) progression. knockdown MM cells was investigated vitro vivo role TUG1. We also predicted transcription factor (TF) that bound together with downstream target genes TUG1-TF interaction, evaluated regulatory mechanism cell assays. reduced cell’s proliferative migratory capabilities while increasing apoptosis bortezomib sensitivity inhibiting tumorigenesis vivo. found nucleus be positively regulated by TF-YY1. Further mechanistic investigations indicated YY1-TUG1 complex targeted YOD1 regulate

Language: Английский

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Identification and characterization of stromal-like cells with CD207+/low CD1a+/low phenotype derived from histiocytic lesions – a perspective in vitro model for drug testing

BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)

Published: Feb. 12, 2024

Abstract Background Histiocytoses are rare disorders manifested by increased proliferation of pathogenic myeloid cells sharing histological features with macrophages or dendritic and accumulating in various organs, i.a., bone skin. Pre-clinical vitro models that could be used to determine molecular pathways the disease limited, hence research on histiocytoses is challenging. The current study compares cytophysiological progenitor, stromal-like derived from histiocytic lesions (sl-pHCs) three pediatric patients different types outcomes. characterized may find potential applications drug testing. Methods Molecular phenotype cells, i.e. expression CD1a CD207 (langerin), was determined using flow cytometry. Cytogenetic analysis included GTG-banded metaphases microarray (aCGH) evaluation. Furthermore, morphology ultrastructure were evaluated a confocal scanning electron microscope. microphotographs imaging reconstruct mitochondrial network its morphology. Basic parameters, such as viability, activity, proliferation, analyzed multiple cellular assays, including Annexin V/7-AAD staining, mitopotential analysis, BrdU test, clonogenicity distribution within cell cycle. Biomarkers potentially associated progression RT-qPCR at mRNA, miRNA lncRNA levels. Intracellular accumulation histiocytosis-specific proteins detected Western blot. Cytotoxicyty IC50 vemurafenib trametinib MTS assay. Results Obtained models, RAB-1, HAN-1, CHR-1, heterogenic terms express CD1a/CD207 markers characteristic for but also show intracellular mesenchymal origin, vimentin (VIM) osteopontin (OPN). In subsequent cultures, remain viable metabolically active, well developed, some distinctive morphotypes noted each line. Cell-specific transcriptome profile noted, providing information new biomarkers (non-coding RNAs) diagnostic prognostic features. showed sensitivity trametinib. Conclusion can studies histiocytosis biology

Language: Английский

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