Dual-responsive near-infrared turn-on fluorescent probe for cancer stem cell-specific visualization

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(28), P. 6959 - 6967

Published: Jan. 1, 2024

Aldehyde dehydrogenase 1A1 (ALDH1A1) stands out as one of the most reliable intracellular biomarkers for stem cells because it is expressed in both cancer (CSCs) and normal somatic (NSCs). Although several turn-on fluorescent probes ALDH1A1 have been developed to visualize CSCs cells, discrimination from NSCs difficult. We here report an AND-type dual-responsive probe, CHO_βgal, near-infrared fluorescence which can be turned on after responding β-galactosidase. The dual responsiveness enables clearly visualized, whereas are non-emissive microscopy. CSC-positive metastasis model lungs were successfully discriminated ex vivo staining experiments using single-input ALDH1A1-responsive probe failed achieve this owing pronounced false-positive output lung NSCs. In tissue slice experiments, even presence adjacent tissues, peripheral region-specific localization was clear. versatility CHO_βgal holds promise not only a fundamental vitro research tool visualizing but also valuable asset practical diagnosis, significantly contributing assessment malignancy.

Language: Английский

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Polyploid giant cancer cells induced by Docetaxel exhibit a senescence phenotype with the expression of stem cell markers in ovarian cancer cells

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0306969 - e0306969

Published: July 11, 2024

Docetaxel (Doc) plays a crucial role in clinical antineoplastic practice. However, it is continuously documented that tumors frequently develop chemoresistance and relapse, which may be related to polyploid giant cancer cells (PGCCs). The aim of this study was investigate the formation mechanism biological behavior PGCCs induced by Doc. Ovarian were treated with Doc, then effect Doc on cellular viability evaluated MTT assay microscopic imaging analysis. properties further Hoechst 33342 staining, cell cycle DNA content assay, damage response (DDR) signaling detection, β-galactosidase mitochondrial membrane potential reverse transcription-quantitative polymerase chain reaction. results indicated reduced viability; however, many still alive, polyploid. increased proportion stayed G2/M phase number cells. In addition, expression γ-H2A.X constantly after treatment. showed senescence-associated activity an increase monomeric form JC-1. mRNA level octamer-binding transcription factor 4 ( OCT4) krüppel-like KLF4) significantly PGCCs. Taken together, our suggest induces arrest, inhibits proliferation activates persistent DDR promote Importantly, exhibit senescence phenotype express stem markers.

Language: Английский

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Tumor-produced immune regulatory factors as a therapeutic target in cancer treatment

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 14, 2024

Citation: Rogovskii V (2024) Tumor-produced immune regulatory factors as a therapeutic target in cancer treatment. Front. Immunol. 15:1416458. doi: 10.3389/fimmu.2024.1416458

Language: Английский

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Cancer Stem Cells in Gastrointestinal Cancers

Stem cell biology and regenerative medicine, Journal Year: 2024, Volume and Issue: unknown, P. 279 - 336

Published: Jan. 1, 2024

Language: Английский

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