Single-cell profiling transcriptomic reveals cellular heterogeneity and cellular crosstalk in breast cancer lymphatic node, bone, and brain metastases

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 17, 2025

Breast cancer is the most common malignant tumor in world, and its metastasis main cause of death breast patients. However, differences between primary tissue lymphatic node, bone, brain metastases at single-cell level are not fully understood. We analyzed microenvironment heterogeneity samples (n = 4), node 3), bone 2) using sequencing data from GEO database. The epithelial cells were characterized by InferCNV algorithm. cell-cell communication was CellChat package. biological function cell subpopulations gene set variation analysis. expression STMN1 immunohistochemical staining. proportion pCAFs explored multispectral identified seven clusters metastatic (Lymphatic brain, metastases) analyzing transcriptomic profiles. T-NK B dominated with metastasis, whereas fibroblasts prevalent cancer. five T (T memory, CD8 + cells, regulatory natural killer CD4 cells), three (naïve memory plasma cancer-associated (CAFs) (Smooth muscle (SMC), pericyte, antigen-presenting CAFs (apCAFs), proliferative (pCAFs), matrix (mCAFs)). Notably. metastasis. Furthermore, we four subpopulations: G0, G1, G2, G3. G2 population exhibited strong invasion ability, it can differentiate into G3 ability proliferation-related G1 after Cell-cell demonstrated an interaction metastasis-associated cells. Finally, discovered that advanced cancer, pCAF increased associated a poor prognosis This study elucidated potential cellular origins drivers to nodes, utilizing prognosis.

Language: Английский

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Thiazolidinedione derivatives in cancer therapy: exploring novel mechanisms, therapeutic potentials, and future horizons in oncology

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Language: Английский

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Single-Cell RNA-Sequencing: Opening New Horizons for Breast Cancer Research

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9482 - 9482

Published: Aug. 31, 2024

Breast cancer is the most prevalent malignant tumor among women with high heterogeneity. Traditional techniques frequently struggle to comprehensively capture intricacy and variety of cellular states interactions within breast cancer. As global precision medicine rapidly advances, single-cell RNA sequencing (scRNA-seq) has become a highly effective technique, revolutionizing research by offering unprecedented insights into heterogeneity complexity This cutting-edge technology facilitates analysis gene expression profiles at level, uncovering diverse cell types microenvironment. By dissecting composition transcriptional signatures cells, scRNA-seq provides new perspectives for understanding mechanisms behind therapy, drug resistance metastasis in In this review, we summarized working principle workflow emphasized major applications discoveries research, highlighting its impact on our comprehension biology potential guiding personalized treatment strategies.

Language: Английский

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The development of the occurrence and metastasis of breast cancer by single-cell sequencing

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 26, 2024

Breast cancer is currently the most frequent malignant tumor and leading cause of death among women globally. Although five-year survival rate for early breast has risen to more than 90%, medication resistance persists in advanced some intractable cancer, resulting a poor prognosis, high recurrence rate, low rate. Single-cell sequencing (SCS) study single cell's gene structure expression level differences order discover unusual molecular subgroups, disease development, variety mechanisms. This review briefly discusses single-cell its application, lists research on development metastasis bring fresh ideas comprehensive treatment cancer.

Language: Английский

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