The lncrna HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis DOI Creative Commons
Jinhua He, Fei Tian, Jie Li

et al.

Journal of Ovarian Research, Journal Year: 2025, Volume and Issue: 18(1)

Published: June 3, 2025

LncRNAs are crucial regulators of ovarian cancer, playing a significant role in malignant transformation and closely linked to poor prognosis. Therefore, it is investigate the impact lncRNAs on biological behavior cancer understand their underlying molecular mechanisms. Gene expression levels were measured using qRT-PCR. The cells was assessed through cell function assays. binding sites target genes predicted bioinformatics analysis, gene targeting relationships verified dual-luciferase reporter gene(DLRG) assay. Protein analyzed Western blotting. In cells, HMMR-AS1 PTN upregulated, whereas miR-627-3p downregulated. Cell experiments demonstrated that could enhance while exhibited opposite effect. DLRG assay indicated lncRNA directly targets miR-627-3p, with identified as miR-627-3p. blotting promoted protein, inhibited it. Furthermore, or partially reverse effects phenotype cells. summary, highly expressed plays carcinogenic by regulating miR-627-3p/PTN axis suggest potential strategy for treatment.

Language: Английский

The lncRNA HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis DOI
Jinhua He, Fei Tian, Jie Li

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract Background Long non-coding RNAs (lncRNAs) are crucial regulators of ovarian cancer(OC), playing a significant role in malignant transformation and closely linked to poor prognosis. Therefore, it is investigate the impact lncRNAs on biological behavior cancer understand their underlying molecular mechanisms. Methods The binding sites target genes were predicted through bioinformatics analysis, gene expression levels measured using qRT-PCR. cells was assessed cell function assays. Gene targeting relationships verified dual-luciferase reporter gene(DLRG) assay. Protein analyzed Western blotting. Results Hyaluronan-mediated motility receptor antisense RNA1(HMMR-AS1) upregulated cells. Cell experiments demonstrated that HMMR-AS1 promotes behaviors DLRG targets miR-627-3p, which turn pleiotrophin(PTN). Furthermore, we discovered functions as competing endogenous RNA (ceRNA) for regulating PTN thereby promoting phenotypes Conclusions In summary, our study indicates lncRNA highly expressed plays carcinogenic role. Targeting may offer novel therapeutic strategy treating cancer.

Language: Английский

Citations

0
The Metabolic Landscape of Cancer Stem Cells: Insights and Implications for Therapy DOI Creative Commons

Martina Milella,

Monica Rutigliano,

Savio Domenico Pandolfo

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(10), P. 717 - 717

Published: May 15, 2025

Cancer stem cells (CSCs) are a subpopulation with self-renewal and differentiation capacities believed to be responsible for tumor initiation, progression, recurrence. These exhibit unique metabolic features that contribute their stemness survival in hostile microenvironments. Like non-stem cancer cells, CSCs primarily rely on glycolysis ATP production, akin the Warburg effect. However, also show increased dependence alternative pathways, such as oxidative phosphorylation (OXPHOS) fatty acid metabolism, which provide necessary energy building blocks therapy resistance. The plasticity of enables them adapt fluctuating nutrient availability hypoxic conditions within tumor. Recent studies highlight importance these shifts maintaining CSC phenotype promoting progression. model suggests small, metabolically adaptable drives growth can switch between mitochondrial enhancing under stress dormant states. Targeting metabolism offers promising therapeutic strategy; however, adaptability complicates eradication. A multi-targeted approach addressing various pathways is essential effective elimination, underscoring need further research into specific markers mechanisms distinguish from normal successful intervention.

Language: Английский

Citations

0
The lncrna HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis DOI Creative Commons
Jinhua He, Fei Tian, Jie Li

et al.

Journal of Ovarian Research, Journal Year: 2025, Volume and Issue: 18(1)

Published: June 3, 2025

LncRNAs are crucial regulators of ovarian cancer, playing a significant role in malignant transformation and closely linked to poor prognosis. Therefore, it is investigate the impact lncRNAs on biological behavior cancer understand their underlying molecular mechanisms. Gene expression levels were measured using qRT-PCR. The cells was assessed through cell function assays. binding sites target genes predicted bioinformatics analysis, gene targeting relationships verified dual-luciferase reporter gene(DLRG) assay. Protein analyzed Western blotting. In cells, HMMR-AS1 PTN upregulated, whereas miR-627-3p downregulated. Cell experiments demonstrated that could enhance while exhibited opposite effect. DLRG assay indicated lncRNA directly targets miR-627-3p, with identified as miR-627-3p. blotting promoted protein, inhibited it. Furthermore, or partially reverse effects phenotype cells. summary, highly expressed plays carcinogenic by regulating miR-627-3p/PTN axis suggest potential strategy for treatment.

Language: Английский

Citations

0