LncRNA-SLC16A1-AS1 induces metabolic reprogramming during Bladder Cancer progression as target and co-activator of E2F1 DOI Creative Commons
Stella Logotheti,

Stephan Marquardt,

Shailendra K. Gupta

et al.

Theranostics, Journal Year: 2020, Volume and Issue: 10(21), P. 9620 - 9643

Published: Jan. 1, 2020

Long non-coding RNAs (lncRNAs) have emerged as integral components of E2F1-regulated gene regulatory networks (GRNs), but their implication in advanced or treatment-refractory malignancy is unknown. Methods: We combined high-throughput transcriptomic approaches with bioinformatics and structure modeling to search for lncRNAs that participate E2F1-activated prometastatic GRNs phenotypic targets the highly-relevant case E2F1-driven aggressive bladder cancer (BC). RNA immunoprecipitation was performed verify RNA-protein interactions. Functional analyses including qRT-PCR, immunoblotting, luciferase assays measurement extracellular fluxes were conducted validate expression target regulation. Results: identified E2F1-responsive lncRNA-SLC16A1-AS1 its associated neighboring protein-coding gene, SLC16A1/MCT1, which both promote invasiveness. Mechanistically, upon E2F1-mediated co-transactivation pair, SLC16A1-AS1 associates E2F1 a structure-dependent manner forms an complex enhances SLC16A1/MCT1 through binding composite SLC16A1-AS1:E2F1-responsive promoter element. Moreover, increases aerobic glycolysis mitochondrial respiration fuels ATP production by fatty acid β-oxidation. These metabolic changes are accompanied alterations PPARA, key mediator Conclusions: Our results unveil new program E2F1-induced transcription factor promotes reprogramming towards acquisition hybrid oxidative phosphorylation/glycolysis cell phenotype favoring BC

Language: Английский

The Role of Non-coding RNAs in Oncology DOI Creative Commons
Frank J. Slack, Arul M. Chinnaiyan

Cell, Journal Year: 2019, Volume and Issue: 179(5), P. 1033 - 1055

Published: Nov. 1, 2019

Language: Английский

Citations

1255
The biology, function, and applications of exosomes in cancer DOI Creative Commons
Jinyi Liu,

Liwen Ren,

Sha Li

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2021, Volume and Issue: 11(9), P. 2783 - 2797

Published: Jan. 10, 2021

Exosomes are cell-derived nanovesicles with diameters from 30 to 150 nm, released upon fusion of multivesicular bodies the cell surface. They can transport nucleic acids, proteins, and lipids for intercellular communication activate signaling pathways in target cells. In cancers, exosomes may participate growth metastasis tumors by regulating immune response, blocking epithelial-mesenchymal transition, promoting angiogenesis. also involved development resistance chemotherapeutic drugs. liquid biopsies be used as non-invasive biomarkers early detection diagnosis cancers. Because their amphipathic structure, natural drug delivery vehicles cancer therapy.

Language: Английский

Citations

372
Exosome-transmitted circular RNA hsa_circ_0051443 suppresses hepatocellular carcinoma progression DOI
Wei Chen,

Yingyao Quan,

Shaoyi Fan

et al.

Cancer Letters, Journal Year: 2020, Volume and Issue: 475, P. 119 - 128

Published: Jan. 31, 2020

Language: Английский

Citations

236
Exosomes: roles and therapeutic potential in osteoarthritis DOI Creative Commons
Zhenhong Ni, Siru Zhou, Song Li

et al.

Bone Research, Journal Year: 2020, Volume and Issue: 8(1)

Published: June 19, 2020

Exosomes participate in many physiological and pathological processes by regulating cell-cell communication, which are involved numerous diseases, including osteoarthritis (OA). detectable the human articular cavity were observed to change with OA progression. Several joint cells, chondrocytes, synovial fibroblasts, osteoblasts, tenocytes, can produce secrete exosomes that influence biological effects of targeted cells. In addition, from stem cells protect damage promoting cartilage repair, inhibiting synovitis, mediating subchondral bone remodeling. This review summarizes roles therapeutic potential discusses perspectives challenges related exosome-based treatment for patients future.

Language: Английский

Citations

227
Long Non-coding RNAs in Cancer: Implications for Diagnosis, Prognosis, and Therapy DOI Creative Commons

Yuchen Qian,

Lei Shi, Zhong Luo

et al.

Frontiers in Medicine, Journal Year: 2020, Volume and Issue: 7

Published: Nov. 30, 2020

Long non-coding RNAs (lncRNAs) are major components of cellular transcripts that arising as important players in various biological pathways. They have received extensive attention recent years, regarded to be involved both developmental processes and diseases. Due their specific expression functional diversity a variety cancers, lncRNAs promising applications cancer diagnosis, prognosis therapy. Studies shown with high specificity accuracy the potential become biomarkers cancers. LncRNAs can noninvasively extracted from body fluids, tissues cells, used independent or auxiliary improve diagnosis prognosis. Currently, most well-recognized lncRNA is PCA3, which has been approved for use prostate cancer. Moreover, underlying mechanisms were explored therapeutic targets, investigated clinical trials several In this review, we presented compilation publications, patents, addressing could considered hopes providing promised implications future

Language: Английский

Citations

209
Recent advances in exosome-mediated nucleic acid delivery for cancer therapy DOI Creative Commons
Ying Zhang, Qiqi Liu, Xinmeng Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: June 14, 2022

Abstract Cancer is a leading public health problem worldwide. Its treatment remains daunting challenge, although significant progress has been made in existing treatments recent years. A large concern the poor therapeutic effect due to lack of specificity and low bioavailability. Gene therapy recently emerged as powerful tool for cancer therapy. However, delivery methods limit its effects. Exosomes, subset extracellular vesicles secreted by most cells, have characteristics good biocompatibility, toxicity immunogenicity, great designability. In past decades, carriers diagnostic markers, they caught extensive attention. This review introduced exosomes, focused on their applications DNA, messenger RNA (mRNA), microRNA (miRNA), small interfering (siRNA), circular (circRNA) other nucleic acids. Meanwhile, application exosome-based clinical trials were presented discussed. Through systematic summarization analysis, advances current challenges exosome-mediated acid are introduced, which will provide theoretical basis development drugs. Graphical

Language: Английский

Citations

191
CircRNA Cdr1as functions as a competitive endogenous RNA to promote hepatocellular carcinoma progression DOI Creative Commons
Yang Su, Xiurui Lv,

Wei Yin

et al.

Aging, Journal Year: 2019, Volume and Issue: 11(19), P. 8183 - 8203

Published: Oct. 1, 2019

Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. Recent years, circular RNA (circRNA) have been shown to exert vital functions in pathological progressions many diseases. A growing number evidences identified representative function exosomal circRNAs physiological state donor cells, which further induces cellular responses after captured by recipient cells. However, contributions HCC remain largely unknown. In vitro and vivo regulatory roles circRNA Cdr1as proliferative migratory abilities were evaluated CCK8, EdU, Transwell tumourigenicity assays, respectively. Results showed was highly expressed cell lines tissues. Overexpression greatly accelerated cells proliferate migrate. Mechanistically, we found that could promote expression AFP, a well-known biomarker for HCC, sponging miR-1270. Further studies exosomes extracted from overexpressing surrounding normal all, serves as ceRNA progression HCC. Meanwhile, it directly transferred via mediate biological

Language: Английский

Citations

189
Long noncoding RNAs and exosomal lncRNAs: classification, and mechanisms in breast cancer metastasis and drug resistance DOI

Hassan Yousefi,

Maryam Maheronnaghsh,

Fatemeh Molaei

et al.

Oncogene, Journal Year: 2019, Volume and Issue: 39(5), P. 953 - 974

Published: Oct. 10, 2019

Language: Английский

Citations

187
Comprehensive landscape of extracellular vesicle-derived RNAs in cancer initiation, progression, metastasis and cancer immunology DOI Creative Commons
Wei Hu, Cong Liu, Zhuoyue Bi

et al.

Molecular Cancer, Journal Year: 2020, Volume and Issue: 19(1)

Published: June 5, 2020

Abstract Extracellular vesicles (EVs), a class of heterogeneous membrane vesicles, are generally divided into exosomes and microvesicles on basis their origination from the endosomal or plasma membrane, respectively. EV-mediated bidirectional communication among various cell types supports cancer growth metastasis. EVs derived different status have been shown to distinct RNA profiles, comprising messenger RNAs non-coding (ncRNAs). Recently, ncRNAs attracted great interests in field EV-RNA research, growing numbers ranging microRNAs long investigated reveal specific functions underlying mechanisms tumor microenvironment premetastatic niches. Emerging evidence has indicated that EV-RNAs essential functional cargoes modulating hallmarks cancers reciprocal crosstalk within cells between stromal over short distance, thereby regulating initiation, development progression cancers. In this review, we discuss current findings regarding EV biogenesis, release interaction with target as well sorting, highlight biological roles molecular EV-ncRNAs biology.

Language: Английский

Citations

179
Exosome-transmitted lncRNA UFC1 promotes non-small-cell lung cancer progression by EZH2-mediated epigenetic silencing of PTEN expression DOI Creative Commons

Xueyan Zang,

Jianmei Gu, Jiayin Zhang

et al.

Cell Death and Disease, Journal Year: 2020, Volume and Issue: 11(4)

Published: April 2, 2020

Abstract Long non-coding RNAs (LncRNAs) have been suggested as important regulators of cancer development and progression in non-small cell lung (NSCLC). Nevertheless, the biological roles clinical significance lncRNA UFC1 NSCLC remain unclear. We detected expression tumor tissues, serum, serum exosomes patients by qRT-PCR. Gene overexpression or silencing were used to examine NSCLC. RNA immunoprecipitation ChIP assays performed evaluate interaction between enhancer zeste homolog 2 (EZH2) binding EZH2 PTEN gene promoter. Rescue study was access importance regulation progression. upregulated high level associated with infiltration. knockdown inhibited proliferation, migration invasion while promoted cycle arrest apoptosis. led opposite effects. Mechanistically, bound mediated its accumulation at promoter region gene, resulting trimethylation H3K27 inhibition expression. growth mouse xenograft models simultaneous depletion reversed this effect. cells derived could promote through transfer UFC1. Moreover, Exosome-transmitted promotes inhibiting via EZH2-mediated epigenetic silencing. Exosome-mediated transmit may represent a new mechanism for provide potential marker diagnosis.

Language: Английский

Citations

147