Next-Generation Sequencing Technology: Current Trends and Advancements

Biology, Journal Year: 2023, Volume and Issue: 12(7), P. 997 - 997

Published: July 13, 2023

The advent of next-generation sequencing (NGS) has brought about a paradigm shift in genomics research, offering unparalleled capabilities for analyzing DNA and RNA molecules high-throughput cost-effective manner. This transformative technology swiftly propelled advancements across diverse domains. NGS allows the rapid millions fragments simultaneously, providing comprehensive insights into genome structure, genetic variations, gene expression profiles, epigenetic modifications. versatility platforms expanded scope facilitating studies on rare diseases, cancer genomics, microbiome analysis, infectious population genetics. Moreover, enabled development targeted therapies, precision medicine approaches, improved diagnostic methods. review provides an insightful overview current trends recent technology, highlighting its potential impact areas genomic research. delves challenges encountered future directions including endeavors to enhance accuracy sensitivity data, novel algorithms data pursuit more efficient, scalable, solutions that lie ahead.

Language: Английский

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Apoptosis Deregulation and the Development of Cancer Multi-Drug Resistance

Cancers, Journal Year: 2021, Volume and Issue: 13(17), P. 4363 - 4363

Published: Aug. 28, 2021

The ability of tumor cells to evade apoptosis is established as one the hallmarks cancer. deregulation apoptotic pathways conveys a survival advantage enabling cancer develop multi-drug resistance (MDR), complex phenotype referring concurrent toward agents with different function and/or structure. Proteins implicated in intrinsic pathway apoptosis, including Bcl-2 superfamily and Inhibitors Apoptosis (IAP) family members, well their regulator, suppressor p53, have been development MDR many types. PI3K/AKT pivotal promoting proliferation often overactive tumors. In addition, microenvironment, particularly factors secreted by cancer-associated fibroblasts, can inhibit reduce effectiveness anti-cancer drugs. this review, we describe main alterations that occur apoptosis-and related promote MDR. We also summarize therapeutic approaches against resistant tumors, targeting small molecule inhibitors IAPs or AKT natural origin may be used monotherapy combination conventional therapeutics. Finally, highlight potential exploitation epigenetic modifications reverse phenotype.

Language: Английский

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Epigenetics of glioblastoma multiforme: From molecular mechanisms to therapeutic approaches

Seminars in Cancer Biology, Journal Year: 2020, Volume and Issue: 83, P. 100 - 120

Published: Dec. 25, 2020

Language: Английский

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Triple negative breast cancer (TNBC): Non-genetic tumor heterogeneity and immune microenvironment: Emerging treatment options

Pharmacology & Therapeutics, Journal Year: 2022, Volume and Issue: 237, P. 108253 - 108253

Published: July 21, 2022

Triple-negative breast cancer (TNBC) is an aggressive subtype characterized by extensive intra-tumoral heterogeneity, and frequently develops resistance to therapies. Tumor heterogeneity lack of biomarkers are thought be some the most difficult challenges driving therapeutic relapse. This review will summarize current therapy for TNBC, studies in treatment relapse, including data from recent single cell sequencing. We discuss changes both transcriptome epigenome we mechanisms regulating immune microenvironment. Lastly, provide new perspective patient stratification, options targeting dysregulation microenvironment TNBC patients.

Language: Английский

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Benzimidazole and its derivatives as cancer therapeutics: The potential role from traditional to precision medicine

Acta Pharmaceutica Sinica B, Journal Year: 2022, Volume and Issue: 13(2), P. 478 - 497

Published: Sept. 21, 2022

Cancer is the second leading cause of mortality globally which remains a continuing threat to human health today. Drug insensitivity and resistance are critical hurdles in cancer treatment; therefore, development new entities targeting malignant cells considered high priority. Targeted therapy cornerstone precision medicine. The synthesis benzimidazole has garnered attention medicinal chemists biologists due its remarkable pharmacological properties. Benzimidazole heterocyclic pharmacophore, an essential scaffold drug pharmaceutical development. Multiple studies have demonstrated bioactivities derivatives as potential anticancer therapeutics, either through specific molecules or non-gene-specific strategies. This review provides update on mechanism actions various structure‒activity relationship from conventional healthcare bench clinics.

Language: Английский

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Anticancer Activity of Natural and Synthetic Chalcones

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(21), P. 11306 - 11306

Published: Oct. 20, 2021

Cancer is a condition caused by many mechanisms (genetic, immune, oxidation, and inflammatory). Anticancer therapy aims to destroy or stop the growth of cancer cells. Resistance treatment theleading cause inefficiency current standard therapies. Targeted therapies are most effective due low number side effects resistance. Among small molecule natural compounds, flavonoids particular interest for theidentification new anticancer agents. Chalcones precursors all have biological activities. The activity chalcones ability these compounds act on targets. Natural chalcones, such as licochalcones, xanthohumol (XN), panduretin (PA), loncocarpine, been extensively studied modulated. Modification basic structure in order obtain with superior cytotoxic properties has performed modulating aromatic residues, replacing residues heterocycles, obtaining hybrid molecules. A huge chalcone derivatives diaryl ether, sulfonamide, amine obtained, their presence being favorable activity. amino group aminochalconesis always antitumor This why molecules different nitrogen hetercycles obtained. From these, azoles (imidazole, oxazoles, tetrazoles, thiazoles, 1,2,3-triazoles, 1,2,4-triazoles) importance identification

Language: Английский

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Machine Learning: A New Prospect in Multi-Omics Data Analysis of Cancer

Frontiers in Genetics, Journal Year: 2022, Volume and Issue: 13

Published: Jan. 27, 2022

Cancer is defined as a large group of diseases that associated with abnormal cell growth, uncontrollable division, and may tend to impinge on other tissues the body by different mechanisms through metastasis. What makes cancer so important incidence rate growing worldwide which can have major health, economic, even social impacts both patients governments. Thereby, early prognosis, diagnosis, treatment play crucial role at front line combating cancer. The onset progression occur under influence complicated some alterations in level genome, proteome, transcriptome, metabolome etc. Consequently, advent omics science its broad research branches (such genomics, proteomics, transcriptomics, metabolomics, forth) revolutionary biological approaches opened new doors comprehensive perception landscape. Due complexities formation development cancer, study underlying has gone beyond just one field arena. Therefore, making connection between resultant data from examining them multi-omics pave way for facilitating discovery novel prognostic, diagnostic, therapeutic approaches. As volume complexity studies are increasing dramatically, use leading-edge technologies such machine learning promising assessments data. Machine categorized subset artificial intelligence aims parsing, classification, pattern identification applying statistical methods algorithms. This acquired knowledge subsequently allows computers learn improve accurate predictions experiences processing. In this context, application learning, computational technology offers opportunities achieving in-depth analysis studies. it be concluded roles fight against

Language: Английский

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piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner

Journal of Translational Medicine, Journal Year: 2022, Volume and Issue: 20(1)

Published: Jan. 29, 2022

Abstract Background Cervical cancer (CC) is one of the most common gynecological tumors that threatens women's health and lives. Aberrant expression PIWI-interacting RNA (piRNA) closely related with a range cancers can serve as tumor promoter or suppressor in proliferation, migration invasion. In this study, aim was not only to discover differential piRNA CC tissue (CC cells) normal cervical (normal epithelium cells), but also investigate biological function action mechanism CC. Methods The DESeq2 approach used estimate fold change between tissue. relative expressions piRNAs (piRNA-20657, piRNA-20497, piRNA-14633 piRNA-13350) m6A methyltransferases/demethylases were detected using RT-qPCR. After intervention METTL14 expression, viability CaSki cells SiHa by CCK8. cell proliferation colony formation assay. Apoptosis rate cycle flow cytometry. Transwell assay performed detect EpiQuik Methylation Quantification Kit evaluate methylation levels. Expression methyltransferase-like protein 14 (METTL14), PIWIL-proteins CYP1B1 RT-qPCR western blot. effect on evaluated dual-luciferase reporter vivo effects assessed nude mice experiments. Results showed high tissues cells, mimic (piRNA-14633 overexpression) promoted viability, invasion cells. Besides, increased levels mRNA stability. dual luciferase assays indicated directed target gene piRNA-14633. Knockdown siRNA attenuated while silencing impaired its expression. overexpression has concentration-dependent characteristics. from experiment growth. Conclusion promotes METTL14/CYP1B1 signaling axis, highlighting important role

Language: Английский

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The application of long-read sequencing in clinical settings

Human Genomics, Journal Year: 2023, Volume and Issue: 17(1)

Published: Aug. 8, 2023

Long-read DNA sequencing technologies have been rapidly evolving in recent years, and their ability to assess large complex regions of the genome makes them ideal for clinical applications molecular diagnosis therapy selection, thereby providing a valuable tool precision medicine. In third-generation duopoly, Oxford Nanopore Technologies Pacific Biosciences work towards increasing accuracy, throughput, portability long-read methods while trying keep costs low. These trades made an attractive use research settings. This article provides overview current limitations explores its potential point-of-care testing health care remote

Language: Английский

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Targeting GPX4 in human cancer: Implications of ferroptosis induction for tackling cancer resilience

Cancer Letters, Journal Year: 2023, Volume and Issue: 559, P. 216119 - 216119

Published: March 7, 2023

Language: Английский

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