Research progress of N1-methyladenosine RNA modification in cancer DOI Creative Commons
Yafeng Liu, Shujun Zhang, Xiaohui Gao

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 30, 2024

Abstract N1-methyladenosine (m1A) is a post-transcriptionally modified RNA molecule that plays pivotal role in the regulation of various biological functions and activities. Especially cancer cell invasion, proliferation cycle regulation. Over recent years, there has been burgeoning interest investigating m1A modification RNA. Most studies have focused on enrichment areas different regions. This review provides comprehensive overview methodologies employed for detection modification. Furthermore, this delves into key players modification, known as “writers,” “erasers,” “readers.” by methyltransferases, or writers, such TRMT6, TRMT61A, TRMT61B, TRMT10C, NML, and, removed demethylases, erasers, including FTO ALKBH1, ALKBH3. It recognized m1A-binding proteins YTHDF1, TYHDF2, TYHDF3, TYHDC1, also “readers”. Additionally, we explore intricate relationship between its regulators their implications development progression specific types cancer, discuss how can potentially facilitate discovery novel approaches diagnosis, treatment, prognosis. Our summary methylated adenosine methods regulatory mechanisms cancers useful insights

Language: Английский

Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway DOI Creative Commons
Zhiqiang He,

Yuhan Zhong,

Parbatraj Regmi

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: March 27, 2024

Abnormal angiogenesis is crucial for gallbladder cancer (GBC) tumor growth and invasion, highlighting the importance of elucidating mechanisms underlying this process. LncRNA (long non-coding RNA) widely involved in malignancy GBC. However, conclusive evidence confirming correlation between lncRNAs GBC lacking.

Language: Английский

Citations

19
RNA m5C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer DOI Creative Commons
Xiaoyi Liu,

Qinglv Wei,

Chenyue Yang

et al.

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(3), P. 600 - 615

Published: March 1, 2024

Abstract 5-Methylcytosine (m 5 C) is a common RNA modification that modulates gene expression at the posttranscriptional level, but crosstalk between m C and biomolecule condensation, as well transcription factor-mediated transcriptional regulation, in ovarian cancer, poorly understood. In this study, we revealed methyltransferase NSUN2 facilitates mRNA forms positive feedback regulatory loop with factor E2F1 cancer. Specifically, promotes of increases its stability, binds to promoter, subsequently reciprocally activating transcription. The binding protein YBX1 functions reader involved NSUN2-mediated regulation. phase separation, which upregulates expression. are amplified upregulated, higher predicts worse prognosis for cancer patients. Moreover, transcriptionally regulates oncogenes MYBL2 RAD54L, driving progression. Thus, our study delineates NSUN2-E2F1-NSUN2 regulated by manner dependent on previously unidentified pathway could be promising target treatment.

Language: Английский

Citations

18
Critical roles and clinical perspectives of RNA methylation in cancer DOI Creative Commons

Ganglei Li,

Qinfan Yao,

Peixi Liu

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(5)

Published: May 1, 2024

Abstract RNA modification, especially methylation, is a critical posttranscriptional process influencing cellular functions and disease progression, accounting for over 60% of all modifications. It plays significant role in metabolism, affecting processing, stability, translation, thereby modulating gene expression cell essential proliferation, survival, metastasis. Increasing studies have revealed the disruption metabolism mediated by methylation has been implicated various aspects cancer particularly metabolic reprogramming immunity. This profound implications tumor growth, metastasis, therapy response. Herein, we elucidate fundamental characteristics their impact on expression. We highlight intricate relationship between reprogramming, immunity, using well‐characterized phenomenon as framework to discuss methylation's specific roles mechanisms progression. Furthermore, explore potential targeting regulators novel approach therapy. By underscoring complex which contributes this review provides foundation developing new prognostic markers therapeutic strategies aimed at treatment.

Language: Английский

Citations

17
The m6A methyltransferase METTL3 drives thyroid cancer progression and lymph node metastasis by targeting LINC00894 DOI Creative Commons
Xiang Zhou, Lisha Chang,

Qiaoqiao Liang

et al.

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 30, 2024

Abstract Background Long noncoding RNAs (lncRNAs) are significant contributors to various human malignancies. The aberrant expression of lncRNA LINC00894 has been reported in We aimed illustrate the role and its underlying mechanism development papillary thyroid carcinoma (PTC). Methods performed bioinformatics analysis differentially expressed from TCGA GEO datasets selected target LINC00894. SRAMP revealed abundant M6A modification sites Further StarBase, GEPIA, was identify related genes METTL3. Colony formation CCK-8 assays confirmed relationship between LINC00894, METTL3, proliferative capacity PTC cells. AnnoLnc2, Starbase datasets, meRIP-PCR qRT‒PCR experiments influence METTL3-mediated m6A on study employed KEGG enrichment as well Western blotting investigate impact proteins Hippo signalling pathway. Results downregulation detected tissues cells even further downregulated with lymphatic metastasis. inhibits lymphangiogenesis vascular endothelial proliferation cancer METTL3 enhances progression by upregulating enhancing mRNA stability through m6A-YTHDC2-dependent may inhibit malignant phenotypes Conclusion METTL3-YTHDC2 axis stabilizes an m6A-dependent manner subsequently tumour malignancy

Language: Английский

Citations

16
Advances in Drug Targeting, Drug Delivery, and Nanotechnology Applications: Therapeutic Significance in Cancer Treatment DOI Creative Commons
Fatih Çiftçi, Ali Can Özarslan, İmran Cagri Kantarci

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(1), P. 121 - 121

Published: Jan. 16, 2025

In the 21st century, thanks to advances in biotechnology and developing pharmaceutical technology, significant progress is being made effective drug design. Drug targeting aims ensure that acts only pathological area; it defined as ability accumulate selectively quantitatively target tissue or organ, regardless of chemical structure active substance method administration. With targeting, conventional, biotechnological gene-derived drugs body’s organs, tissues, cells can be transported specific regions. These systems serve carriers regulate timing release. Despite having many advantageous features, these have limitations thoroughly treating complex diseases such cancer. Therefore, combining with nanoparticle technologies imperative treat cancer at both local systemic levels effectively. The nanocarrier-based delivery involves encapsulating target-specific molecules into polymeric vesicular systems. Various (DDS) were investigated discussed this review article. first part passive systems, hydrogels, thermoplastics, microdevices transdermal-based second carrier nanobiotechnology (carbon nanotubes, nanoparticles, coated, pegylated, solid lipid nanoparticles smart nanogels). third part, advantages discussed, finally, market research commercial used nanotechnological approaches was included.

Language: Английский

Citations

2
m6A Demethylase FTO-Mediated Upregulation of BAP1 Induces Neuronal Ferroptosis via the p53/SLC7A11 Axis in the MPP+/MPTP-Induced Parkinson’s Disease Model DOI

Zhengyu Li,

Xin Chen,

Wenwen Xiang

et al.

ACS Chemical Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Background: Parkinson's disease (PD) is a neurodegenerative disorder characterized by the involvement of ferroptosis in its pathological mechanism. In this study, effects and mechanism BRCA1-associated protein 1 (BAP1) on neuronal PD were evaluated. Methods: A mouse model was constructed injecting mice with MPTP. Nissl staining, immunohistochemistry, immunofluorescence, Prussian blue staining evaluated histopathology iron distribution. The cell subjecting SK-N-SH cells to MPP+. m6A level BAP1 assessed MeRIP. mRNA levels BAP1, FTO, IGF2BP1, METTL3, YTHDF2, SLC7A11 utilizing RT-qPCR. Protein SLC7A11, p53 measured Western blot. Cell viability using CCK-8 assay, TUNEL used for assessing apoptosis. MDA, GSH, SOD, Fe2+ also measured. interactions among molecules verified RIP dual luciferase reporter ChIP assay. Results: treated MPP+ showed decrease overall BAP1. FTO facilitated demethylation leading an increased expression m6A-binding protein, YTHDF2 recognized decayed methylated reduced stability. FTO/BAP1 axis promoted MPP+-induced suppressing SLC7A11. collaboration p53, Knocking down mitigated MPTP model. Conclusion: m6A-mediated modification regulates cooperating Thus, may be potential therapeutic target treatment.

Language: Английский

Citations

2
Epitranscriptome: Review of Top 25 Most-Studied RNA Modifications DOI Open Access
Viktoriia A. Arzumanian, Georgii Dolgalev, Ilya Y. Kurbatov

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(22), P. 13851 - 13851

Published: Nov. 10, 2022

The alphabet of building blocks for RNA molecules is much larger than the standard four nucleotides. diversity achieved by post-transcriptional biochemical modification these nucleotides into distinct chemical entities that are structurally and functionally different from their unmodified counterparts. Some modifications constituent critical functions, while others serve as dynamic markings to regulate fate specific molecules. Together, form epitranscriptome, an essential layer cellular biochemistry. As time writing this review, more 300 all three life domains have been identified. However, only a few most well-established included in reviews on topic. To provide complete overview current state research we analyzed extent available information known modifications. We selected 25 describe detail. Summarizing our findings, status identify further developments field.

Language: Английский

Citations

45
m6A writer WTAP targets NRF2 to accelerate bladder cancer malignancy via m6A-dependent ferroptosis regulation DOI
Ke Wang, Gang Wang, Gang Li

et al.

APOPTOSIS, Journal Year: 2023, Volume and Issue: 28(3-4), P. 627 - 638

Published: Jan. 31, 2023

Language: Английский

Citations

38
METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progression DOI Creative Commons
Wei Wei, Zhongyuan Zhang, Bin Shi

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)

Published: June 20, 2023

Abstract Background Glycolysis is the key hallmark of cancer and maintains malignant tumor initiation progression. The role N6-methyladenosine (m6A) modification in glycolysis largely unknown. This study explored biological function m6A methyltransferase METTL16 glycolytic metabolism revealed a new mechanism for progression Colorectal (CRC). Methods expression prognostic value was evaluated using bioinformatics immunohistochemistry (IHC) assays. functions CRC analyzed vivo vitro. Glycolytic assays were used to verify Suppressor glucose by autophagy (SOGA1). protein/RNA stability, RNA immunoprecipitation (RIP), Co-immunoprecipitation (Co-IP) pull-down explore potential molecular mechanisms. Results SOGA1 direct downstream target involved mediated significantly enhances mRNA stability via binding “reader” protein insulin-like growth factor 2 1 (IGF2BP1). Subsequently, promotes AMP-activated kinase (AMPK) complex ubiquitination, inhibits its phosphorylation, thus upregulates pyruvate dehydrogenase 4 (PDK4), crucial controlling metabolism. Moreover, Yin Yang (YY1) can transcriptionally inhibit cells directly promoter. Clinical data showed that positively correlated PDK4, associated with poor prognosis patients. Conclusions Our findings suggest METTL16/SOGA1/PDK4 axis might be promising therapeutic targets CRC.

Language: Английский

Citations

31
The emerging importance role of m6A modification in liver disease DOI Open Access

Sheng Wang,

Songsen Gao,

Wufei Ye

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 162, P. 114669 - 114669

Published: April 8, 2023

N6-methyladenosine (m6A) modification, as one of the most common types inner RNA modification in eukaryotes, plays a multifunctional role normal and abnormal biological processes. This type is modulated by m6A writer, eraser reader, which turn impact various processes metabolism, such processing, translation, nuclear export, localization decay. The current academic view holds that exerts crucial post-transcriptional modulation gene expression, involved multiple cellular functions, developmental disease However, potential molecular mechanism specific development liver have not been fully elucidated. In our review, we summarized latest research progress on disease, explored how these novel findings reshape knowledge metabolism. addition, also illustrated effect regeneration to prompt further exploration physiology pathology, providing new insights references for search therapeutic targets disease.

Language: Английский

Citations

30