Three-Dimensional Visualization of Breast Cancer Pathology Evolution in Clinical Patient Tissues with NIR-II Imaging DOI
Shasha Li, Xin Qi, Yuan Li

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(33), P. 10337 - 10347

Published: Aug. 9, 2024

Breast cancer (BC) is the most common tumor worldwide and requires crucial molecular typing for treatment prognosis assessment. Currently, approaches like pathological staining, immunohistochemistry (IHC), immunofluorescence (IF) face limitations due to low signal-to-background ratio (SBR) high heterogeneity, resulting in a misdiagnosis rate. Fluorescent assay second near-infrared region (NIR-II, 1000–1700 nm) exhibits ultrahigh SBR owing diminished scattering tissue autofluorescence. Here, we present NIR-II strategy accurate BC three-dimensional (3D) visualization based on atomically precise fluorescent Au24Pr1 clusters. Single-atom Pr doping results 3.9-fold fluorescence enhancement long-term photostability. The clusters possess centered at ∼1100 nm section diagnosis was 4 times higher than that of NIR-I imaging. This enables spatial resolution 3D biopsy specimens, which can surmount heterogeneity clinical BC.

Language: Английский

Exploring the prognostic and predictive potential of bacterial biomarkers in non-gastrointestinal solid tumors DOI Creative Commons

Caoimbhe Burke,

Thomas J. Glynn, Chowdhury Arif Jahangir

et al.

Expert Review of Molecular Diagnostics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Standard clinical parameters like tumorsize, age, lymph node status, and molecular markers are used to predictprogression risk treatment response. However, exploring additional markersthat reflect underlying biology could offer a more comprehensive understandingof the tumor microenvironment (TME). The TME influences development,progression, disease severity, survival, with tumor-associated bacteriaposited play significant roles. Studies on microbiota havefocused high bacterial-load sites such as gut, oral cavity, stomach,but interest is growing in non-gastrointestinal (GI) solid tumors, asbreast, lung, pancreas. Microbe-based biomarkers, including Helicobacter pylori, humanpapillomavirus (HPV), hepatitis B C viruses, have proven valuable inpredicting gastric, cervical, renal cancers. Potential of prognostic andpredictive bacterial biomarkers non-GI tumors methodologiesused. Advances techniques 16SrRNA gene sequencing, qPCR, immunostaining, situ hybridization enabled detailed analysis ofdifficult-to-culture microbes tumors. ensure reliableresults, it critical standardize protocols, accurately align reads,address contamination, maintain proper sample handling. This will pave theway for developing reliable that enhance prognosis,prediction, personalized planning.

Language: Английский

Citations

0
Reducing the availability of endogenous copper and glucose for cascade starvation therapy and chemodynamic therapy DOI Creative Commons
Chunhui Wang, Pingting Ye,

Mengyao Chen

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101702 - 101702

Published: March 1, 2025

Language: Английский

Citations

0
Three-Dimensional Visualization of Breast Cancer Pathology Evolution in Clinical Patient Tissues with NIR-II Imaging DOI
Shasha Li, Xin Qi, Yuan Li

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(33), P. 10337 - 10347

Published: Aug. 9, 2024

Breast cancer (BC) is the most common tumor worldwide and requires crucial molecular typing for treatment prognosis assessment. Currently, approaches like pathological staining, immunohistochemistry (IHC), immunofluorescence (IF) face limitations due to low signal-to-background ratio (SBR) high heterogeneity, resulting in a misdiagnosis rate. Fluorescent assay second near-infrared region (NIR-II, 1000–1700 nm) exhibits ultrahigh SBR owing diminished scattering tissue autofluorescence. Here, we present NIR-II strategy accurate BC three-dimensional (3D) visualization based on atomically precise fluorescent Au24Pr1 clusters. Single-atom Pr doping results 3.9-fold fluorescence enhancement long-term photostability. The clusters possess centered at ∼1100 nm section diagnosis was 4 times higher than that of NIR-I imaging. This enables spatial resolution 3D biopsy specimens, which can surmount heterogeneity clinical BC.

Language: Английский

Citations

2