Quantifying the ∼75-95% of Peptides in DIA-MS Datasets that were not Previously Quantified

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract We demonstrate an algorithm termed GoldenHaystack (GH) that, compared to the leading DIA-MS algorithm, (a) quantifies and identifies with better FDR accuracy peptides found in FASTA search spaces (∼5-25% of analytes datasets), (b) remaining ∼75-95% that were previously unquantified, (c) runs ∼40-200x faster (or ∼1-10x than LC-MS). Specifically, without using a file or spectral library, GH can deconvolute accurately quantify chimeric LC-MS spectra. The central idea enables this claim is: for sufficiently sized projects (e.g., ≥ ∼50 files), pairs co-elute one subset files do not exactly different files. thus analyzes project holistically: it uses multi -partite matching match fragment ions across all samples, separates regroups into distinct peptide signatures, then those signatures.

Language: Английский

Construction of AMPK-related circRNA network in mouse myocardial ischemia–reperfusion injury model

BMC Cardiovascular Disorders, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 30, 2024

To screen Myocardial ischemia-reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI.

Language: Английский