Folic Acid-Targeted Mixed Pluronic Micelles for Delivery of Triptolide DOI Open Access
Meizhen Yin, Xinying Zhang,

T.Y. Zhang

et al.

Polymers, Journal Year: 2024, Volume and Issue: 16(24), P. 3485 - 3485

Published: Dec. 13, 2024

The present study aimed to explore an ideal delivery system for triptolide (TPL) by utilizing the thin-film hydration method prepare drug-loaded, folate-modified mixed pluronic micelles (FA–F-127/F-68–TPL). Scanning electron microscopy and atomic force showed that drug-loaded had a spherical shape with small particle size, average of 30.7 nm. Cell viability experiments FA–F-127/F-68–TPL significantly reduced HepG2 cell viability, exhibiting strong cytotoxicity. Its cytotoxicity was markedly enhanced compared bare TPL. Nile red (Nr) used as model drug FA–F-127/F-68–Nr further validate its tumor-targeting cellular uptake capability. After coincubation cells, multifunctional microplate reader intracellular fluorescence intensity increased, indicating could more effectively enter cells. A nude mouse subcutaneous hepatocellular carcinoma constructed. Following tail vein injection FA–F-127/F-68–Nr, imaging FA–F127/F-68–Nr target tumor tissue, even if entering small-sized challenging, it be excreted through urine. Nude mice were treated injections (45 µg/kg) every other day 21 days. results growth transplanted tumors slowed, no significant difference In summary, exhibits advantages low cost, excellent biological properties, active/passive targeting capabilities, notable against liver cancer inhibition growth. Significantly, FA–F-127/F-68–TPL, despite challenges in insignificant EPR effect, can efficiently via kidneys, thereby preventing release during prolonged circulation potential damage normal tissues. Therefore, represents promising antitumor system.

Language: Английский

Folic Acid-Targeted Mixed Pluronic Micelles for Delivery of Triptolide DOI Open Access
Meizhen Yin, Xinying Zhang,

T.Y. Zhang

et al.

Polymers, Journal Year: 2024, Volume and Issue: 16(24), P. 3485 - 3485

Published: Dec. 13, 2024

The present study aimed to explore an ideal delivery system for triptolide (TPL) by utilizing the thin-film hydration method prepare drug-loaded, folate-modified mixed pluronic micelles (FA–F-127/F-68–TPL). Scanning electron microscopy and atomic force showed that drug-loaded had a spherical shape with small particle size, average of 30.7 nm. Cell viability experiments FA–F-127/F-68–TPL significantly reduced HepG2 cell viability, exhibiting strong cytotoxicity. Its cytotoxicity was markedly enhanced compared bare TPL. Nile red (Nr) used as model drug FA–F-127/F-68–Nr further validate its tumor-targeting cellular uptake capability. After coincubation cells, multifunctional microplate reader intracellular fluorescence intensity increased, indicating could more effectively enter cells. A nude mouse subcutaneous hepatocellular carcinoma constructed. Following tail vein injection FA–F-127/F-68–Nr, imaging FA–F127/F-68–Nr target tumor tissue, even if entering small-sized challenging, it be excreted through urine. Nude mice were treated injections (45 µg/kg) every other day 21 days. results growth transplanted tumors slowed, no significant difference In summary, exhibits advantages low cost, excellent biological properties, active/passive targeting capabilities, notable against liver cancer inhibition growth. Significantly, FA–F-127/F-68–TPL, despite challenges in insignificant EPR effect, can efficiently via kidneys, thereby preventing release during prolonged circulation potential damage normal tissues. Therefore, represents promising antitumor system.

Language: Английский

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