Alterations in the oral microbiome and metabolome of methamphetamine addicts DOI Creative Commons
Dawei Wang,

Yu Feng,

Min Yang

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 19, 2023

Abstract Background : Drug addiction can seriously damage human physical and mental health, while detoxification is a long difficult process. Although studies have reported changes in the oral microbiome of methamphetamine (METH) addicts, role plays this process still unknown. This study aims to explore function based on analysis variations metabolome METH addicts. Results We performed 16S rRNA sequencing saliva samples collected from 278 addicts 105 healthy controls (CTL) undergoing at center Shandong, China. In addition, untargeted metabolomic profiling was conducted 220 (170 50 CTL) identify biomarkers build classifiers for both microbiota metabolites. Compared CTL group, alpha diversity reduced group with significant differences metabolic pathways, including enhanced tryptophan metabolism, lysine biosynthesis, purine steroid biosynthesis. Conversely, pathways porphyrin glutathione pentose phosphate were significantly reduced. It speculated that four key microbial taxa, i.e., Peptostreptococcus , Gemella Campylobacter Aggregatibacter could be involved toxicity mechanisms by affecting above pathways. prediction models more effective than metabolite-based identifying addiction. Conclusions: Our identified potential functional connections between profile providing novel insights into exploring toxic underlying

Language: Английский

Gut microbiota changes associated with low-carbohydrate diet intervention for obesity DOI Creative Commons
Li Li,

Xiaoguo Zhao,

Rashidin Abdugheni

et al.

Open Life Sciences, Journal Year: 2024, Volume and Issue: 19(1)

Published: Jan. 1, 2024

Abstract Low-carbohydrate diets (LCDs) are frequently recommended for alleviating obesity, and the gut microbiota plays key roles in energy metabolism weight loss. However, there is limited in-human research on how LCD changes microbiota. In this before–after study, 43 participants were assigned to intervention 4 weeks. The main objective was investigate specific that occur participants’ microbiome response LCD. Changes analyzed using 16s rRNA sequencing. Body composition measured InBody 770. Remarkably, 35 (79.07%) lost more than 5% of their body weight; levels BMI, fat, total cholesterol significantly decreased, indicating effectiveness intervention. richness increased after By taking intersection ANOVA linear discriminant analysis effect size (LEfSe) results, we identified three phyla, classes, four orders, five families, six genera differentially enriched between baseline week-4 time points. Among relative abundances Firmicutes Actinobacteriota decreased significantly, while Bacteroidetes significantly. At genus level, Ruminococcus , Agathobacter Streptococcus Bifidobacterium showed a significant reduction abundances, whereas Parabacteroides Bacteroides steadily. Our results demonstrate can effectively alleviate obesity modify certain taxa microbiota, providing potential insights personalized dietary interventions against obesity.

Language: Английский

Citations

3
Anti-obesogenic effect of lupin-derived protein hydrolysate through modulation of adiposopathy, insulin resistance and gut dysbiosis in a diet-induced obese mouse DOI Open Access
Eduardo Ponce‐España, Iván Cruz-Chamorro, Guillermo Santos‐Sánchez

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 178, P. 117198 - 117198

Published: July 26, 2024

The prevalence of obesity is increasingly widespread, resembling a global epidemic. Lifestyle changes, such as consumption high-energy-dense diets and physical inactivity, are major contributors to obesity. Common features this metabolic pathology involve an imbalance in lipid glucose homeostasis including dyslipidemia, insulin resistance adipose tissue dysfunction. Moreover, the importance gut microbiota development susceptibility has recently been highlighted. In recent years, new strategies based on use functional foods, particular bioactive peptides, have proposed counteract outcomes. context, present study examines effects lupin protein hydrolysate (LPH) obesity, dyslipidemia dysbiosis mice fed high-fat diet (HFD). After 12 weeks LPH treatment, gained less weight showed decreased dysfunction compared HFD-fed group. HFD-induced (increased triglycerides, cholesterol LDL concentration) were both counteracted by consumption. Discriminant analysis differentially distributed LPH-treated non-treated mice. HFD reduced ecological parameters, promoted blooming deleterious taxa abundance commensal members. Some these changes corrected Finally, correlation suggested that microbial population could be responsible for improvement conclusion, first show effect improving gain, adiposopathy context diet-induced pointing therapeutic potential peptides diseases.

Language: Английский

Citations

3
In vitro gastrointestinal digestion of whole grain noodles supplemented with soluble dietary fiber and their effects on children fecal microbiota DOI
Linlin Gao, Lin Zhang, Huan Liu

et al.

Food Bioscience, Journal Year: 2023, Volume and Issue: 53, P. 102600 - 102600

Published: March 30, 2023

Language: Английский

Citations

8
A polysaccharide from edible red seaweed Bangia fusco-purpurea prevents obesity in high-fat diet-induced C57BL/6 mice DOI
Mingjing Zheng, Xiaopei Chao,

Yajun Zheng

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 283, P. 137545 - 137545

Published: Nov. 13, 2024

Language: Английский

Citations

2
Qushi Huayu decoction ameliorates non-alcoholic fatty liver disease in rats by modulating gut microbiota and serum lipids DOI Creative Commons
Yiming Ni, Xin Wang, Qian Wu

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 11, 2023

Non-alcoholic fatty liver disease (NAFLD) is a multifactorial disease. As clinical empirical prescription of traditional Chinese medicine, Qushi Huayu decoction (QHD) has attracted considerable attention for its advantages in multi-target treatment NAFLD. However, the intervention mechanism QHD on abnormal lipid levels and gut microbiota NAFLD not been reported.Therefore, we verified therapeutic effect high-fat diet (HFD)-induced rats by physiological parameters histopathological examination. In addition, studies serum lipidomics based 16S rRNA sequencing ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) were conducted to elucidate QHD.The changes are mainly reflected their diversity composition, while treated restored these changes. The genera Blautia, Lactobacillus, Allobaculum, Lachnoclostridium Bacteroides predominant group, whereas, Turicibacter, Sporosarcina, Romboutsia, Clostridium_sensu_stricto_1, Psychrobacter NAFLD+QHD group. Lipid subclasses, including diacylglycerol (DG), triglycerides (TG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidic acid (PA), phosphatidylserine (PS), lysophosphatidylinositol (LPI), phosphatidylglycerol (PG), significantly different between control groups, altered DG, TG, PA, lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), platelet activating factor (PAF). Finally, Spearman's correlation analysis showed that related differential molecules associated with Bacteroides, Lachnoclostridium, which also correlated biological NAFLD.Taken together, may exert beneficial effects regulating thus intervening lipids.

Language: Английский

Citations

6
Pediococcus acidilactici Y01 reduces HFD-induced obesity via altering gut microbiota and metabolomic profiles and modulating adipose tissue macrophage M1/M2 polarization DOI
Yujing Wang, Yu Xue, Huan Xu

et al.

Food & Function, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

P. acidilactici Y01 has the anti-obesity effect possibly via altering gut microbiota and metabolomic profiles modulating adipose tissue macrophage M1/M2 polarization.

Language: Английский

Citations

1
Variations in the oral microbiome and metabolome of methamphetamine users DOI Creative Commons
Dawei Wang, Yu Feng, Min Yang

et al.

mSystems, Journal Year: 2023, Volume and Issue: 9(1)

Published: Dec. 19, 2023

Drug addiction can seriously damage human physical and mental health, while detoxification is a long difficult process. Although studies have reported changes in the oral microbiome of methamphetamine (METH) users, role that plays process drug still unknown. This study aims to explore function based on analysis variations metabolome METH users. We performed 16S rRNA sequencing saliva samples collected from 278 users 105 healthy controls (CTL). In addition, untargeted metabolomic profiling was conducted 220 samples. Compared CTL group, alpha diversity reduced group relative abundances

Language: Английский

Citations

3
Effect of vitamin D3 on lipid droplet growth in adipocytes of mice with HFD‐induced obesity DOI Creative Commons
Jingjing Zhang, Tracy Zhang, Yong Zhou

et al.

Food Science & Nutrition, Journal Year: 2023, Volume and Issue: 11(10), P. 6686 - 6697

Published: Aug. 24, 2023

Vitamin D-regulating action of PPARγ on obesity has been confirmed adipocyte differentiation. However, it is not clear whether vitamin D affects the morphological size mature adipocytes by influencing expression in vivo. Our hypothesis was that D3 (VitD3) inhibits growth suppressing white obese mice. Five-week-old male C57BL/6J mice were randomly divided into normal diet and high-fat groups. After 10 weeks, body weight between two groups differed 26.91%. The a diet, solvent control, low-dose VitD3 (5000 IU/kg·food), medium-dose (7500 high-dose (10,000 PPAR γ antagonist group, intervention lasted for 8 weeks. Diet-induced (DIO) fed exacerbated markers adiposity (body weight, fat mass, mass rate, brown adipocytes, mRNA, protein levels ATGL Fsp27), level Fsp27 decreased group. In conclusion, possibly via inhibiting expression, thereby lipolysis, increasing volume decreasing their fat-storing ability resulted expression. Therefore, long-term oral may necessarily improve obesity, we need more clinical trials to explore dose duration treatment deficiency patients.

Language: Английский

Citations

2
Multi-trajectories of body mass index, waist circumference, gut microbiota, and incident dyslipidemia: a 27-year prospective study DOI Creative Commons
Xiaofan Zhang,

Fangxu Guan,

Wanglong Gou

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 19, 2024

Evidence is insufficient to establish a longitudinal association between combined trajectories of body mass index (BMI) and waist circumference (WC) dyslipidemia. Our study aimed explore the multi-trajectories BMI WC incident dyslipidemia identify microbiota metabolite signatures these trajectories.

Language: Английский

Citations

0
The serum metabolic profile plays a role in mediating the regulatory impact of gut microbiota and its metabolites on susceptibility to obesity in mice DOI Creative Commons
Jingrong Huang,

Baoting Chen,

Yuhang Wen

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Abstract Obesity is a multifaceted health concern that impacted by genetic, environmental, and behavioral factors, with varying levels of individual susceptibility. Research has identified distinct physiological characteristics among individuals differing susceptibilities to obesity. Prior research demonstrated notable variances in gut microbiota metabolites between obesity-prone (OP) those obesity-resistance (OR). However, the potential impact these differences on serum remains uncertain, as does role metabolite changes influencing susceptibility We performed non-targeted metabolomic analysis samples from OP OR mice. Subsequently, correlation was conducted data pertaining metabolites, microbiota, obesity-related indicators, other factors. This study aimed investigate association alterations mice, well examine Our demonstrates following HFD, 8Z,11Z,14Z-Eicosatrienoic acid (ESA) Eicosapentaenoic (EPA) can lipid through regulation Arachidonic (AA) EPA. EPA also influences abundance Lachnospiraceae_NK4A136_group, resulting increased accumulation. Moreover, Ruminiclostridium_9 exacerbates saturated fatty acids AA decreasing Additionally, Hydroxybenzoic accumulation elevating Choline levels. These contribute development obesity, indicating variations play significant disparities, being influenced metabolites.

Language: Английский

Citations

0