Rapid screening and sensing of stearoyl-CoA desaturase 1 (SCD1) inhibitors from ginger and their efficacy in ameliorating non-alcoholic fatty liver disease

Journal of Food Measurement & Characterization, Journal Year: 2024, Volume and Issue: 18(8), P. 6843 - 6857

Published: June 21, 2024

Abstract Non-alcoholic fatty liver disease is a prevalent chronic metabolic condition, for which no approved medications are available. As condiment and traditional Chinese medicine, ginger can be useful in reducing the symptoms of non-alcoholic disease. Although its active ingredients mechanisms action unknown, there lack research on them. The purpose this study to prepare magnetite (Fe 3 O 4 )@Stearoyl-CoA desaturase 1 (SCD1) materials analyze them using ultra-high performance liquid-chromatography-mass spectrometry (UPLC-MS) rapid screening potential inhibitors SCD1 ginger. Based analysis, it has been shown that primary components bind directly gingerols, with 10-gingerol having greater affinity binding than 8-gingerol 6-gingerol. Moreover, further studies indicated free acids (FFA)-induced lipid accumulation improved by class compounds normal human hepatocytes (THLE-3), being most effective compound. This provides new insight into mechanism, contributes improvement (NAFLD) provide support use treatment NAFLD.

Language: Английский

Targeting lipid metabolic reprogramming to alleviate diabetic kidney disease: molecular insights and therapeutic strategies

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

Diabetic kidney disease (DKD) is one of the major complications diabetes, and its pathological progression closely associated with lipid metabolic reprogramming. Under diabetic conditions, renal cells undergo significant abnormalities, including increased uptake, impaired fatty acid oxidation, disrupted cholesterol efflux, enhanced catabolism, as adaptive responses to stress. These changes result in accumulation lipids such free acids, diacylglycerol, ceramides, leading lipotoxicity that triggers inflammation fibrosis. Hypoxia DKD microenvironment suppresses oxidation promotes synthesis through HIF-1α pathway, while chronic exacerbates disturbances via inflammatory cytokines, inflammasomes, macrophage polarization. Targeting metabolism represents a promising therapeutic strategy for alleviating DKD; however, further clinical translational studies are warranted validate efficacy safety these approaches.

Language: Английский

Lianweng Granules Alleviate Intestinal Barrier Damage via the IL-6/STAT3/PI3K/AKT Signaling Pathway with Dampness-Heat Syndrome Diarrhea

Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 661 - 661

Published: May 28, 2024

Dampness-heat syndrome diarrhea (DHSD) is a common clinical disease with high prevalence but still has no satisfactory therapeutic medicine, so the search for safe and effective drug candidate ongoing. This study aims to explore efficacy mechanisms of Lianweng granules (LWG) in treatment DHSD identify blood transport components LWG. We assessed LWG by various vivo metrics such as body weight, activity index (DAI), histopathologic examination, intestinal barrier function, levels inflammatory, apoptotic biomarkers, oxidative stress. identified using ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UHPLC-MS/MS), resolved key were used relevant targets. next predicted potential treating network pharmacology molecular docking based on Finally, validated RT-qPCR, Western blotting, ELISA, immunofluorescence evaluated vitro Cell Counting Kit-8 (CCK-8), small interfering RNA, cellular enthusiasm transfer assay (CETSA), affinity response target stability (DARTS). Ninety-one pharmacodynamic enter bloodstream exert possible effects. In vivo, improved reduced colonic injury DAI scores, lowered inflammation, stress, apoptosis markers, partially restored function mice. Guided docking, it suggested that may effects inhibiting IL-6/STAT3/PI3K/AKT signaling. significantly decreased expression IL-6, p-STAT3, p-PI3K, p-AKT, other proteins. These findings supported experiments, where CETSA, DARTS, siRNA evidenced LWG’s targeting STAT3. targeted STAT3 inhibit colon, thereby restoring some extent exerting effect DHSD.

Language: Английский