Comparaison des performances diagnostiques de deux biopuces IgE : ISAC® et ALEX2® DOI Creative Commons

S. Lecolant,

D. Khelifi,

Catherine Neukirch

et al.

Revue française d'allergologie, Journal Year: 2023, Volume and Issue: 63(2), P. 103289 - 103289

Published: Jan. 25, 2023

Specific IgE to individual allergen components: Peanut DOI
Anna M. Ehlers, Edward F. Knol, Stef J. Koppelman

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 495 - 506

Published: Jan. 1, 2024

Language: Английский

Citations

0
Multiparametric or multiplex systems in allergy diagnostics DOI

Roberto Bernardini,

Stefania Arasi, Simona Barni

et al.

Rivista di immunologia ed allergologia pediatrica., Journal Year: 2024, Volume and Issue: 38(2), P. 17 - 21

Published: June 1, 2024

Language: Английский

Citations

0
Comparative studies of seafood and reptile α‐ and β‐parvalbumins DOI
Andrea O’Malley,

Joshua M. Ray,

Paweł Kitlas

et al.

Protein Science, Journal Year: 2024, Volume and Issue: 33(12)

Published: Nov. 25, 2024

Abstract Small calcium‐binding proteins such as parvalbumins (PVs) are major seafood and fish allergens. However, the impact of structural changes on their capacity to bind IgE has not been studied in detail. Therefore, reptilian PVs, well human α‐PV, were selected for biochemical, structural, binding studies. Likely due high solubility, crystallization proved difficult, so additional techniques used promote proteins. Novel crystal structures determined PV, cod allergen Gad m 1.0201, saltwater crocodile Cro p 1.0101, α‐PV from thornback ray. β‐PVs considered allergens, while α‐PVs rarely categorized To explain these differences, results studies combined. This approach allowed us provide new insight into epitopes present focusing cross‐reactivity among α‐ β‐PVs. In addition, we have shown that display remarkable thermal stability across a range pH conditions, which is relevant case food allergens processing. Moreover, it presence calcium cations critical PVs via protein folding, an formation epitopes. These shows reptile PV allows further evaluation cross‐reactivity.

Language: Английский

Citations

0
Molecular Profiles of Sensitization to Non-Specific Lipid Transfer Proteins in Lithuania: Single Center Experience DOI Open Access
Sandra Sakalauskaitė,

Ligita Pilkyte,

Edita Gasiūnienė

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13535 - 13535

Published: Dec. 18, 2024

Non-specific Lipid Transfer proteins (nsLTPs) are relevant allergens of several pollens and plant foods. Sensitization to nsLTPs is not typical in our region. Still, it has become an increasingly common cause IgE-mediated food allergies food-induced anaphylaxis Northern Europe recent decades. No in-depth studies describe the prevalence sensitization molecular components Lithuania. This study aimed determine profile atopic patients at Immunology Allergy Department Kauno Klinikos nsLTPs, using allergen component analysis. Sixty Lithuanian adults with symptoms allergic rhinitis and/or asthma were included into study. Specific immunoglobulin E (IgE) levels measured two vitro techniques: extract Results showed that 25% subjects sensitized nsLTP-containing sources, mostly Zea m 14, Mal d 3, Vit v 1, Art 3. The median amount total IgE was higher nsLTP-sensitized than nsLTP-nonsensitized patients. Based on Cohen’s Kappa McNemar tests, results analysis tests do always agree, especially when we sources containing nsLTPs. Molecular could be first choice determining detailed who experienced unknown origin.

Language: Английский

Citations

0
Comparaison des performances diagnostiques de deux biopuces IgE : ISAC® et ALEX2® DOI Creative Commons

S. Lecolant,

D. Khelifi,

Catherine Neukirch

et al.

Revue française d'allergologie, Journal Year: 2023, Volume and Issue: 63(2), P. 103289 - 103289

Published: Jan. 25, 2023

Citations

1