Pyroptosis in Spinal Cord Injury: Implications for Pathogenesis and Therapeutic Approaches DOI Creative Commons
Guangjin Gu,

Huaqi Yu,

Huishuang Zou

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(6), P. 210 - 210

Published: June 11, 2024

Traumatic spinal cord injury (SCI) is a serious disease of the central nervous system. Aside from limited intrinsic regenerative capacity neurons, complex microenvironmental disturbances can also lead to further cellular damage and growth inhibition. Programmed cell death regulated by pyroptosis has an important role in pathogenesis SCI. While there been wealth new knowledge regarding pyroptosis, detailed understanding its SCI possible therapeutic strategies still lacking. This review summarizes current advances regulatory pyroptosis-regulated inflammasome components inhibitory microenvironment following SCI, as well recent advances.

Language: Английский

Treg cells-derived exosomes promote blood-spinal cord barrier repair and motor function recovery after spinal cord injury by delivering miR-2861 DOI Creative Commons

Guang Kong,

Xiong Wu,

Cong Li

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Oct. 4, 2023

Abstract The blood-spinal cord barrier (BSCB) is a physical between the blood and spinal parenchyma. Current evidence suggests that disruption of BSCB integrity after injury can lead to secondary injuries such as edema excessive inflammatory response. Regulatory T (Treg) cells are effective anti-inflammatory inhibit neuroinflammation injury, their infiltration exhibits same temporal spatial characteristics automatic repair BSCB. However, few studies have assessed relationship Treg emphasizing integrity. This study explored whether affects recovery SCI underlying mechanism. We confirmed angiogenesis cell occurred simultaneously SCI. Furthermore, we observed significant effects on motor function in mice by knockout overexpression. Subsequently, demonstrated presence exosomes vitro. In addition, found cell-derived encapsulated miR-2861, miR-2861 regulated expression vascular tight junction (TJs) proteins. luciferase reporter assay negative regulation IRAK1 series rescue experiments validated biological IRAKI regulating summary, could package deliver regulate affect mice, which provides novel insights for functional limiting inflammation

Language: Английский

Citations

11
From hope to healing: Exploring the therapeutic potential of exosomes in spinal cord injury DOI Creative Commons
Nidhi Singh, Lahanya Guha, Hemant Kumar

et al.

Extracellular Vesicle, Journal Year: 2024, Volume and Issue: 3, P. 100044 - 100044

Published: May 13, 2024

Spinal cord injury (SCI) is the damage to spinal causing paralysis below site compromised patient life quality. Exosomes are nanosized vesicles released during physiological or pathological processes. They present a promising avenue for therapeutic drug delivery due their tissue specificity and biocompatibility, offering enhanced efficacy with reduced accumulation-based toxicity. In SCI, exosomes target inflammation, apoptosis, microglial polarization, autophagy, contributing interventions. This article comprehensively explores pathophysiology of diverse exosome synthesis processes, limitations challenges overcome, potential from various cellular sources in SCI treatment.

Language: Английский

Citations

4
Yin-Yang: two sides of extracellular vesicles in inflammatory diseases DOI Creative Commons
Bin Zeng, Ying Li,

Nawaz Khan

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 27, 2024

The concept of Yin-Yang, originating in ancient Chinese philosophy, symbolizes two opposing but complementary forces or principles found all aspects life. This can be quite fitting the context extracellular vehicles (EVs) and inflammatory diseases. Over past decades, numerous studies have revealed that EVs exhibit dual sides, acting as both pro- anti-inflammatory agents, akin to Yin-Yang theory (i.e., sides a coin). has enabled serve potential indicators pathogenesis manipulated for therapeutic purposes by influencing immune pathways. review delves into recent advances understanding their regulation specific We shed light on current prospects engineering treating conditions. principle bestows upon them great as, therapeutic, preventive agents

Language: Английский

Citations

4
Microglial C/EBPβ-Fcgr1 regulatory axis blocking inhibits microglial pyroptosis and improves neurological recovery DOI Creative Commons
Jing Li, Yubing Yang, Chenguang Zhao

et al.

Journal of Neuroinflammation, Journal Year: 2025, Volume and Issue: 22(1)

Published: Jan. 31, 2025

CAAT/Enhancer Binding Protein β (C/EBPβ) is associated with inflammatory responses in neurodegenerative pathologies, particularly the brain. However, regulatory role of C/EBPβ spinal cord injury and its impact on neurological recovery remain unknown. In this study, we observed significant upregulation microglia after mice was neuroinflammation. Knocking down attenuated pyroptosis, reduced production proinflammatory cytokines, inhibited neuronal apoptosis. Mechanistically, promoted transcription Fcgr1, which involved activating pyroptosis. both in-vivo in-vitro experiments, knocking Cebpb or pyroptosis inhibitor VX765 apoptosis improved mice. These findings indicate that functions as a key regulator participates pyroptosis-mediated neuroinflammation by Fcgr1 transcription.

Language: Английский

Citations

0
T-regulatory cells for the treatment of autoimmune diseases DOI Creative Commons

Marina Fisher,

С. В. Сенников

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 4, 2025

Autoimmune diseases result from imbalances in the immune system and disturbances mechanisms of tolerance. T-regulatory cells (Treg) are key factors formation Tregs modulate responses repair processes, controlling innate adaptive system. The use treatment autoimmune began with manipulation endogenous using immunomodulatory drugs. Then, a method adoptive transfer grown vitro was developed. Adoptive includes polyclonal non-specific effects antigen-specific form CAR-Treg TCR-Treg. This review discusses approaches to Tregs, their advantages, disadvantages, gaps development, future prospects.

Language: Английский

Citations

0
FOXO3-induced microRNA-128-3p promotes the progression of spinal cord injury in mice via regulating NLRP3 inflammasome-mediated pyroptosis DOI Creative Commons
Shuo Yang,

Yunzhi Guan,

Chaojun Zheng

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 21, 2025

Spinal cord injury (SCI) remains a severe condition with an extremely high disability rate and complex pathophysiologic mechanisms. Pyroptosis, inflammatory form of cell death triggered by certain inflammasomes, has key role in variety diseases, including SCI. However, it is unclear whether microRNAs (miRNAs), novel regulators the SCI, are involved SCI-induced pyroptosis. Two GEO miRNA expression profiles (GSE158195 GSE90452) were downloaded, differentially expressed miRNAs analyzed bioinformatics methods. An vivo animal model vitro cellular SCI constructed female C57BL/6 mice BV-2 cells for studying possible roles FOXO3, miR-128-3p NLRP3-mediated pyroptosis Markers ROS, inflammation measured RT-qPCR, Western blotting, immunofluorescence, flow cytometry, enzyme-linked immunosorbent assays. Histopathological changes spinal tissue detected using hematoxylin eosin immunohistochemical. The Basso-Beattie-Bresnahan (BBB) score was used to evaluate motor function each group. Bioinformatics analysis GSE158195 GSE90452 datasets revealed significant downregulation miR-128-3p, phenomenon that consistently observed model. Functionally, upregulation improved functional behavioral recovery, relieved pathological injury, repressed oxidative stress, alleviated mouse models. We also confirmed Thioredoxin-interacting protein (TXNIP) target gene overexpression TXNIP can effectively reverse improvement Moreover, we found transcription factor FOXO3 facilitated expression, its resulted similar effects To best our knowledge, this first report demonstrating secondary through modulation pathway. Our results suggest FOXO3/miR-128-3p/TXNIP/NLRP3-mediated axis may be potential therapeutic

Language: Английский

Citations

0
IsoalloLCA-intervened regulatory T cell exosomes alleviate inflammatory bowel disease by inhibiting NF-κB-associated inflammation in intestinal epithelial cells DOI
Sim Ray Yue, Ling Gong,

Yulin L. Tan

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 154, P. 114501 - 114501

Published: April 12, 2025

Language: Английский

Citations

0
Mesenchymal stem cell exosomes therapy for the treatment of traumatic brain injury: mechanism, progress, challenges and prospects DOI Creative Commons
Ming-wei Liu, Hua Li,

Gang Xiong

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 11, 2025

Language: Английский

Citations

0
Bone marrow mesenchymal stem cells-derived exosomal miR-24-3p alleviates spinal cord injury by targeting MAPK9 to inhibit the JNK/c-Jun/c-Fos pathway DOI

Dongzi Li,

Xiaoyan Xie, Yuxuan Ou

et al.

Archives of Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: 769, P. 110434 - 110434

Published: April 22, 2025

Language: Английский

Citations

0
Regulatory T cell-derived exosome mediated macrophages polarization for osteogenic differentiation in fracture repair DOI

Tingting Wu,

Lulu Wang,

Jian Chen

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 369, P. 266 - 282

Published: March 29, 2024

Language: Английский

Citations

3