Recent applications and molecular mechanisms of hyaluronic acid in skin aging and wound healing

Medicine in Novel Technology and Devices, Journal Year: 2024, Volume and Issue: 23, P. 100320 - 100320

Published: July 14, 2024

Skin aging and wound healing have always been significant topics in the skin field. Hyaluronic acid (HA), a natural glycosaminoglycan (GAG) presenting extracellular matrix (ECM), is extensively applied fields of anti-aging therapeutics repair due to its unique physicochemical biological properties. HA indispensable regulation diverse processes such as cellular proliferation, differentiation, migration, signaling, inflammatory responses, angiogenesis, tissue regeneration. Consequently, HA-based formulations, including creams, gels, serums, dermal fillers, are instrumental combating wrinkles, enhancing hydration, correcting tear trough deformity, restoring facial volume. Additionally, inherent biocompatibility high biodegradability facilitates chemical modification, rendering it an ideal material for This review summarizes recent advances applications molecular mechanisms derivatives field healing; discusses prospects challenges compounds

Language: Английский

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Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application

Gels, Journal Year: 2025, Volume and Issue: 11(1), P. 40 - 40

Published: Jan. 5, 2025

To improve wound healing, advanced biofabrication techniques are proposed here to develop customized patches release bioactive agents targeting cell function in a controlled manner. Three-dimensional (3D) bioprinted "smart" patches, based on methacrylated gellan gum (GGMA), loaded with tannic acid (TA) or L-ascorbic (AA) have been manufactured. stability and degradation time, (GG) was chemically modified by grafting methacrylic moieties the polysaccharide backbone. GGMA were characterized through physicochemical, morphological mechanical evaluation. Kinetics antioxidant potential of TA AA as well antimicrobial activity against common pathogens Pseudomonas aeruginosa, Staphylococcus aureus Escherichia coli accordance ISO 22196:2011 reported. The cytocompatibility demonstrated direct indirect tests human dermal fibroblasts (HDF) per 10993. positive effect migration assessed healing assay. results highlighted that cytocompatible, speed up can swell upon contact hydration medium way. Overall, TA- AA-loaded suitable features; no cytotoxicity; antioxidant, properties, showing satisfactory for dressing applications.

Language: Английский

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Advances of exosomes in diabetic wound healing

Burns & Trauma, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 1, 2025

Poor wound healing is a refractory process that places an enormous medical and financial burden on diabetic patients. Exosomes have recently been recognized as crucial players in the of lesions. They excellent stability, homing effects, biocompatibility, reduced immunogenicity novel cell-free therapies. In addition to transporting cargos target cells enhance intercellular communication, exosomes are beneficial nearly every phase healing. participate modulating inflammatory response, accelerating proliferation reepithelization, increasing angiogenesis, regulating extracellular matrix remodeling. Accumulating evidence indicates hydrogels or dressings conjunction with can prolong duration exosome residency wounds. This review provides overview mechanisms, delivery, clinical application, engineering, existing challenges use repair. We also propose future directions for biomaterials incorporating exosomes: 2D 3D scaffolds, loaded healing-promoting gases, intelligent biomaterials, prospect systematic application exosomes. These findings may might shed light treatments enlighten some studies improve quality life among diabetes

Language: Английский

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Chitosan/cellulose nanocrystals/graphene oxide scaffolds as a potential pH-responsive wound dressing: Tuning physico-chemical, pro-regenerative and antimicrobial properties

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134643 - 134643

Published: Aug. 13, 2024

Chronic wounds (CWs) treatment still represents a demanding medical challenge. Several intrinsic physiological signals (i.e., pH) help to stimulate and support wound healing. CWs, in fact, are characterized by predominantly alkaline pH of the exudate, which acidifies as heals. Therefore, pH-responsive dressings hold great potential owing their capability tuning functions according conditions. Herein, porous chitosan (CS)-based scaffolds loaded with cellulose nanocrystals (CNCs) graphene oxide (GO) were successfully fabricated using freeze-drying method. CNCs extracted from bagasse pulps fibers through acid hydrolysis. GO was synthesised Hummer's The then ionically cross-linked amino L-Arginine (Arg), bioactive agent, tested dressing. Notably, effect singly simultaneously within CS-Arg investigated. modulation content facilitated development an optimal pH-dependent swelling ratio extended degradation time. Furthermore, CS/CNC/GO-Arg exhibited tuned biological features, terms antimicrobial activity, cellular proliferation/migration ability, expression extracellular matrix specific markers elastin collagen I) related healing human dermal fibroblasts.

Language: Английский

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Development and Characterization of Biocompatible Chitosan-Aloe Vera Films Functionalized with Gluconolactone and Sorbitol for Advanced Wound Healing Applications

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Chitosan (CTS) has emerged as a promising biopolymer for wound healing due to its biocompatibility, biodegradability, and intrinsic bioactive properties. This study explores the development characterization of CTS-based films enhanced with natural agents, aloe vera (A), gluconolactone (GL), sorbitol (S), improve their mechanical, antimicrobial, regenerative performance potential use in advanced care. A series were fabricated varying concentrations A, GL, S, physicochemical, biological properties comprehensively evaluated. Fourier transform infrared (FTIR) spectroscopy atomic force microscopy (AFM) analysis revealed modifications film structure attributable these additives, influencing surface roughness, hydrophilicity, thermal stability. Biocidal assays confirmed antimicrobial activity, particularly containing GL A. Biodegradation studies demonstrated significant enhancement microbial decomposition films, while cytocompatibility tests minimal cytotoxic effects improved cellular response. research underscores combining CS S engineer multifunctional biomaterials tailored effectively tackling different phases process, offering sustainable biocompatible alternative clinical applications.

Language: Английский

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Bioprinted Hydrogels as Vehicles for the Application of Extracellular Vesicles in Regenerative Medicine

Gels, Journal Year: 2025, Volume and Issue: 11(3), P. 191 - 191

Published: March 8, 2025

Three-dimensional bioprinting is a new advance in tissue engineering and regenerative medicine. Bioprinting allows manufacturing three-dimensional (3D) structures that mimic tissues or organs. The bioinks used are mainly made of natural synthetic polymers must be biocompatible, printable, biodegradable. These may incorporate progenitor cells, favoring graft implantation regeneration injured tissues. However, the natures biomaterials, processes, lack vascularization, immune responses factors limit viability functionality implanted cells damaged limitations can addressed by incorporating extracellular vesicles (EV) into bioinks. Indeed, EV from have capacities, being similar to those their source cells. Therefore, combinations with biomaterials cell-free therapies. Likewise, they complement manufacture increasing viability, differentiation, ability incorporated Thus, main objective this review show how use 3D technology for application medicine these nanovesicles hydrogels as To end, latest advances derived vitro vivo studies been described. Together, high therapeutic potential strategy

Language: Английский

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Effects of Hydrogels on Mesenchymal Stem/Stromal Cells Paracrine Activity and Extracellular Vesicles Production

Journal of Extracellular Vesicles, Journal Year: 2025, Volume and Issue: 14(3)

Published: March 1, 2025

ABSTRACT Mesenchymal stem/stromal cells (MSCs) are a valuable source of paracrine factors, as they have remarkable secretory capacity, and there is sizeable knowledge base to develop industrial clinical production protocols. Promising cell‐free approaches for tissue regeneration immunomodulation driving research towards secretome applications, among which extracellular vesicles (EVs) steadily gaining attention. However, the manufacturing application EVs limited by insufficient yields, gaps, low standardization. Facing these limitations, hydrogels represent versatile three‐dimensional (3D) culture platform that can incorporate matrix (ECM) components mimic natural stem cell environment in vitro; via niche‐mimicking properties, regulate MSCs’ morphology, adhesion, proliferation, differentiation secretion capacities. impact hydrogel's architectural, biochemical biomechanical properties on remains poorly understood, field still its infancy interdependency parameters compromises comparability studies. Therefore, this review summarizes discusses reported effects hydrogel encapsulation MSC‐EVs. Considering cell‐material interactions overall activity MSCs, we identify persistent challenges from standardization process control, outline future paths research, such synergic use bioreactors enhance MSC‐EV generation.

Language: Английский

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Antiadhesive Hyaluronic Acid-Based Wound Dressings Promote Wound Healing by Preventing Re-Injury: An In Vivo Investigation

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 510 - 510

Published: Feb. 23, 2024

Wound dressings are widely used to protect wounds and promote healing. The water absorption antifriction properties of important for regulating the moisture balance reducing secondary damages during dressing changes. Herein, we developed a hyaluronic acid (HA)-based foam prepared via lyophilization photocrosslinked HA hydrogels with high antiadhesion properties. To fabricate HA-based (HA foam), hydroxyl groups were modified methacrylate groups, enabling rapid photocuring. resulting photocured solution was freeze-dried form porous structure, enhancing its exudate capacity. Compared conventional biopolymer-based dressings, this exhibited superior assess wound-healing potential foam, animal experiments involving SD rats conducted. Full-thickness defects measuring 2 × cm2 created on skin 36 rats, divided into four 9 individuals each. treated gauze, CollaDerm®, CollaHeal® Plus, respectively. closely monitored period 24 days. In vivo testing demonstrated that facilitated wound healing without causing inflammatory reactions minimized This research presents promising biocompatible based HA, which offers enhanced capabilities improved patient comfort addresses challenges associated dressings.

Language: Английский

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Bioactivation of Konjac Glucomannan Films by Tannic Acid and Gluconolactone Addition

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(35), P. 46102 - 46112

Published: Aug. 20, 2024

Wound healing is a dynamic process that requires an optimal extracellular environment, as well accurate synchronization between various cell types. Over the past few years, great efforts have been devoted to developing novel approaches for treating and managing burn injuries, sepsis, chronic or accidental skin injuries. Multifunctional smart-polymer-based dressings represent promising approach support natural address several problems plaguing partially healed including severe inflammation, scarring, wound infection. Naturally derived compounds offer unique advantages such minimal toxicity, cost-effectiveness, outstanding biocompatibility along with potential anti-inflammatory antimicrobial activity. Herein, main driving idea of work was design development konjac glucomannan d-glucono-1,5-lactone (KG) films bioactivated by tannic acid (GL) addition. Our analysis, using attenuated total reflectance-Fourier transform infrared, atomic force microscopy, surface energy measurements demonstrated (TA) clearly interacted KG matrix, acting its cross-linker, whereas GL embedded within polymer structure. All developed maintained moist which represents pivotal property dressing. Hemocompatibility experiments showed all tested exhibited no hemolytic impact on human erythrocytes. Moreover, presence TA enhanced metabolic energetic activity in dermal fibroblasts, indicated MTT assay, showing results exceeding 150%. Finally, high antibacterial properties they significantly reduced multiplication rate both Staphylococcus aureus Escherichia coli bacterial broth created inhibition zones S. agar plates. These remarkable outcomes make KG/TA/GL film candidates applications.

Language: Английский

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Exploring Synergistic Effects of Bioprinted Extracellular Vesicles for Skin Regeneration

Biomedicines, Journal Year: 2024, Volume and Issue: 12(7), P. 1605 - 1605

Published: July 18, 2024

Regenerative medicine represents a paradigm shift in healthcare, aiming to restore tissue and organ function through innovative therapeutic strategies. Among these, bioprinting extracellular vesicles (EVs) have emerged as promising techniques for rejuvenation. EVs are small lipid membrane particles secreted by cells, known their role potent mediators of intercellular communication the exchange proteins, genetic material, other biological components. The integration 3D technology with offers novel approach engineering, enabling precise deposition EV-loaded bioinks construct complex three-dimensional (3D) architectures. Unlike traditional cell-based approaches, bioprinted eliminate need live thereby mitigating regulatory financial obstacles associated cell therapy. By leveraging synergistic effects bioprinting, researchers aim enhance outcomes skin regeneration while addressing current limitations conventional treatments. This review explores evolving landscape transformative regeneration. Furthermore, it discusses challenges future directions harnessing this therapy clinical applications, emphasizing interdisciplinary collaboration continued scientific inquiry unlock its full potential.

Language: Английский

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