Frontiers in Endocrinology,
Journal Year:
2024,
Volume and Issue:
15
Published: Dec. 10, 2024
As
the
population
ages,
occurrence
of
osteoporosis
is
becoming
more
common.
Diabetes
mellitus
one
factors
in
development
osteoporosis.
Compared
with
general
population,
incidence
significantly
higher
diabetic
patients.
Diabetic
(DOP)
a
metabolic
bone
disease
characterized
by
abnormal
tissue
structure
due
to
hyperglycemia
and
insulin
resistance,
reduced
strength
increased
risk
fractures.
This
complex
mechanism
that
occurs
at
cellular
level
such
as
blood
vessels,
inflammation,
resistance.
Although
application
some
drugs
clinical
practice
can
reduce
DOP,
fractures
caused
DOP
still
very
high.
Extracellular
vesicles
(EVs)
are
new
communication
mode
between
cells,
which
transfer
miRNAs
proteins
from
mother
cells
target
through
membrane
fusion,
thereby
regulating
function
cells.
In
recent
years,
role
EVs
pathogenesis
has
been
widely
demonstrated.
this
article,
we
first
describe
changes
microenvironment
Second,
DOP.
Finally,
summarize
research
progress
challenges
Materials Today Bio,
Journal Year:
2025,
Volume and Issue:
31, P. 101509 - 101509
Published: Jan. 22, 2025
Articular
cartilage,
composed
of
chondrocytes
within
a
dynamic
viscoelastic
matrix,
has
limited
self-repair
capacity,
posing
significant
challenge
for
regeneration.
Constructing
high-fidelity
cartilage
organoids
through
three-dimensional
(3D)
bioprinting
to
replicate
the
structure
and
physiological
functions
is
crucial
regenerative
medicine,
drug
screening,
disease
modeling.
However,
commonly
used
matrix
bioinks
lack
reversible
cross-linking
precise
controllability,
hindering
cellular
regulation.
Thus,
encoding
adaptive
cultivating
an
attractive
idea.
DNA,
with
its
ability
be
intricately
encoded
reversibly
cross-linked
into
hydrogels,
offers
manipulation
at
both
molecular
spatial
structural
levels.
This
endows
hydrogels
viscoelasticity,
printability,
cell
recognition,
stimuli
responsiveness.
paper
elaborates
on
strategies
encode
bioink
via
emphasizing
regulation
predictable
properties
resulting
interactions
behavior.
The
significance
these
construction
highlighted.
Finally,
we
discuss
challenges
future
prospects
using
DNA-encoded
3D
bioprinted
organoids,
underscoring
their
potential
impact
advancing
biomedical
applications.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 14, 2025
Integrin
and
focal
adhesion
can
regulate
cytoskeleton
distribution
to
govern
actin-related
force
remodeling
play
an
important
role
in
nuclear
configuration
force-sensing
mechanotransduction
of
stem
cells.
However,
further
exploration
the
interaction
between
actinin
complex
myosin,
kinetics,
molecular
mechanism
structures
nucleate
within
engineered
cells
is
vague.
An
extensive
comprehension
cell
morphogenesis,
remodeling,
essential
reveal
basic
physical
principles
polymerization
force-related
signaling
delivery.
Advanced
microarrays
are
designed
determine
heterogeneous
morphology
behaviors
The
heterogeneity
from
transferred
into
nuclei
by
evaluation
Lamins,
YAP,
BrdU
expression.
Tuning
activation
proteins
nucleators
adjust
mechanics
may
be
underlying
change
response
its
physiological
microarrayed
Osteoporosis
(OP)
is
a
systemic
skeletal
disorder
characterized
by
decreased
bone
mineral
density
and
heightened
risk
of
fractures.
Therapies
for
OP
have
primarily
focused
on
balancing
formation
resorption,
but
enhancing
the
remineralization
osteoporotic
also
key
strategy
effective
repair.
Recent
insights
into
biomineralization
mechanisms
highlighted
essential
role
mineral-containing
extracellular
vesicles
(EVs)
secreted
osteoblasts
in
promoting
marrow
mesenchymal
stromal/stem
cell
(BMSC)
differentiation
initiating
matrix
mineralization.
Drawing
from
these
principles,
we
developed
biomimetic
approach
to
replicate
structure
function
osteoblast-derived
EVs
engineering
mitochondrial
minerals
with
homing
membranes
(CMs).
This
bone-targeted
system
exhibits
excellent
biocompatibility,
osteogenic
stimulating
angiogenesis
regulating
cellular
energy
metabolism.
Additionally,
CM-coated
shows
affinity
collagen
fibrils,
effectively
intrafibrillar
mineralization,
thereby
facilitating
Overall,
offers
safe
efficient
therapeutic
alternative,
positioning
it
as
platform
tissue
regenerative
medicine.
Polymers for Advanced Technologies,
Journal Year:
2025,
Volume and Issue:
36(2)
Published: Feb. 1, 2025
ABSTRACT
The
objective
of
a
regenerative
medicine
is
to
repair,
restore,
and
regenerate
tissues
organs
that
occurred
due
injury
or
defect
disease.
Additionally,
the
purpose
reverse
aging
process
body
by
utilizing
body's
innate
healing
ability.
There
still
significant
venture
needs
be
directed
for
advancement
in
arena
medicine,
which
makes
use
products
derived
from
cell
therapy
as
well
biomedical.
Stem
cells
growth
factors
are
primary
components
medicine;
hence,
novel
drug
delivering
systems
now
being
studied
augment
transport
active
pharmaceuticals.
Lipid‐based
complexed
with
therapeutics
suited
site‐specific
controlled
release.
Lipids
biocompatible
available
various
sources
molecular
weights
self‐assemble
into
forms
hydrophilic
hydrophobic
parts.
By
enhancing
plasma
membrane
fluidity,
excipients
such
non‐ionic
surfactants,
fatty
acids,
glycerides
increase
permeability.
Liposomes,
nanoparticles,
nano‐emulsions,
micelles
nano‐lipid
carriers
can
improve
bioavailability
both
lipophilic
medicines.
lipoidal‐polymeric
had
emerged
promising
multifaceted
application
cytocompatibility,
ecofriendly,
ability
encapsulate
variety
drugs.
Further,
treatment
biomedical
tissue
engineering,
rapidly
developing
discipline.
Furthermore,
similar
vein,
delivery
technologies
combined
stem
promote
engraftment,
differentiation,
survival.
Hence
colloidal
carrier
has
impending
versatile
applications.
Therefore,
review
offer
an
abridged
information
on
significance
technology
offering
confined
ecosystem
facilitates
them
thrive
distinguish
effective
manner.
International Journal of Nanomedicine,
Journal Year:
2025,
Volume and Issue:
Volume 20, P. 2059 - 2071
Published: Feb. 1, 2025
Osteoporosis,
a
critical
public
health
challenge,
is
marked
by
skeletal
deformities
and
heightened
fracture
risk.
Steleophaga
plancyi
(Boleny)
(SP),
component
of
traditional
Chinese
medicine,
known
to
enhance
bone
health,
but
the
molecular
mechanisms
behind
its
osteoprotective
effects
are
not
well
understood.
We
isolated
extracellular
vesicle
(EV)-like
particles
from
SP
(SP-EVLP)
using
differential
velocity
centrifugation
investigated
their
on
human
marrow
stromal
cells
(hBMSCs)
in
vitro.
utilized
CCK-8,
Alkaline
phosphatase
(ALP)
alizarin
red
staining
(ARS),
RNA-seq,
bioinformatics,
immunofluorescence,
Western
blot
elucidate
role
SP-EVLP.
The
therapeutic
potential
SP-EVLP
was
evaluated
an
ovariectomized
(OVX)
rat
model,
standard
model
for
osteoporosis,
encapsulating
them
enteric-coated
capsules.
were
successfully
characterized,
they
shown
be
effectively
internalized
hBMSCs,
enhancing
osteogenic
differentiation.
In
OVX
encapsulated
capsules
significantly
increased
mass,
indicating
robust
effect.
Further
mechanistic
studies
revealed
that
promotes
osteoblast
proliferation
activating
melatonin-induced
autophagy,
pathway
may
improve
osteoporotic
conditions.
Our
results
establish
as
promising
candidate
osteoporosis.
activation
autophagy
suggests
mechanism
effects,
opening
new
possibilities
osteoporosis
treatment
development.
Osteoporosis,
a
condition
marked
by
reduced
bone
mass
and
structural
deterioration,
continues
to
be
major
public
health
concern,
especially
as
global
populations
age.
Excessive
osteoclast
formation
is
hallmark
of
osteoporosis.
The
transcription
factor
nuclear
activated
T-cells
cytoplasmic
1
(NFATc1)
indispensable
for
the
early
differentiation
osteoclasts,
orchestrating
expression
essential
genes,
while
at
later
stages,
cathepsin
K
(CTSK)
resorption
activities
mature
osteoclasts.
Here,
we
fabricated
ultrasound-responsive
microdroplets
(MDs)
modulating
both
stages
functions
osteoclasts
via
targeting
NFATc1
CTSK.
internalization
these
dual
MDs
was
evaluated
in
human
marrow-derived
mesenchymal
stromal
cells
(hBMSCs)
murine
RAW
264.7
macrophages,
alongside
biocompatibility
assay.
Their
effects
on
osteogenesis
osteoclastogenesis
were
further
investigated
vitro,
followed
vivo
analysis
osteoporotic
rat
models.
exhibited
well-defined
core-shell
structure
demonstrated
efficient
cellular
uptake
with
minimal
cytotoxicity.
Furthermore,
showed
effect
osteogenic
hBMSCs.
In
vitro
assays,
effectively
suppressed
through
synergistic
inhibitory
effect.
studies
that
rats
receiving
significant
protection
against
loss
induced
ovariectomy.
These
results
highlight
potential
sophisticated,
targeted
therapeutic
approach
osteoporosis
treatment.
