Cartilage Endplate‐Targeted Engineered Exosome Releasing and Acid Neutralizing Hydrogel Reverses Intervertebral Disc Degeneration DOI

Jiawen Zhan,

Yongzhi Cui, Ping Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 14(2)

Published: Nov. 18, 2024

Abstract Cartilage endplate cell (CEPC) and nucleus pulposus (NPC) inflammation are critical factors that contribute to intervertebral disc degeneration (IVDD). Recent evidence indicated iron ion influx, reactive oxygen species (ROS), the cGAS‐STING pathway involved in CEPC inflammatory degeneration. Moreover, cytokines produced by degenerating CEPCs lactic acid accumulation within microenvironment significantly NPC inflammation. Consequently, simultaneous alleviation of correction acidic anticipated reverse IVDD. Herein, CEPC‐targeted engineered exosomes loaded with salvianolic A incorporated into a CaCO 3 /chitosan hydrogel, forming composite gel, CAP‐sEXOs@Gel. Notably, CAP‐sEXOs@Gel shows long local retention, realizes slow release CAP‐sEXOs specific uptake CEPCs. After CEPCs, reduce intracellular ROS inhibiting hypoxia‐inducible factor‐2α (HIF‐2α)/TfR1 expression. Iron influx inhibition maintenance normal mitochondrial function reduced mtDNA leakage, suppresing pathway. Additionally, component neutralizes H + , thereby alleviating Collectively, this novel hydrogel demonstrates ability concurrently inhibit inflammation, presenting promising therapeutic approach for

Language: Английский

Targeted delivery of extracellular vesicles: the mechanisms, techniques and therapeutic applications DOI Creative Commons
Shuang Zhao,

Yunfeng Di,

Huilan Fan

et al.

Molecular Biomedicine, Journal Year: 2024, Volume and Issue: 5(1)

Published: Nov. 21, 2024

Abstract Extracellular vesicles (EVs) are cell-derived with a phospholipid bilayer measuring 50–150 nm in diameter demonstrated therapeutic potentials. Limitations such as the natural biodistribution (mainly concentrated liver and spleen) short plasma half-life of EVs present significant challenges to their clinical translation. In recent years, growing research indicated that engineered enhanced targeting lesion sites have markedly promoted efficacy. However, there is dearth systematic knowledge on advances engineering for targeted delivery. Herein, we provide an overview mechanisms, techniques, translations applications. Enrichment at may be achieved through recognition tissue markers, pathological changes, circumvention mononuclear phagocyte system (MPS). Alternatively, external stimuli, including magnetic fields ultrasound, also employed. EV techniques fulfill functions includes genetic engineering, membrane fusion, chemical modification physical modification. A comparative statistical analysis was conducted elucidate discrepancies between diverse size, morphology, stability, efficacy vitro vivo. Additionally, summary registered trials utilizing from 2010 2023 has been provided, full discussion perspectives. This review provides comprehensive mechanisms associated delivery applications advocate further explorations accelerate

Language: Английский

Citations

7
Bioengineered extracellular vesicles presenting PD-L1 and Gal-9 to ameliorate new-onset primary ovarian insufficiency (POI) DOI

Yuanyuan Gu,

Guannan Zhou,

Menglei Zhang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162635 - 162635

Published: April 1, 2025

Language: Английский

Citations

0
Cartilage Endplate‐Targeted Engineered Exosome Releasing and Acid Neutralizing Hydrogel Reverses Intervertebral Disc Degeneration DOI

Jiawen Zhan,

Yongzhi Cui, Ping Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 14(2)

Published: Nov. 18, 2024

Abstract Cartilage endplate cell (CEPC) and nucleus pulposus (NPC) inflammation are critical factors that contribute to intervertebral disc degeneration (IVDD). Recent evidence indicated iron ion influx, reactive oxygen species (ROS), the cGAS‐STING pathway involved in CEPC inflammatory degeneration. Moreover, cytokines produced by degenerating CEPCs lactic acid accumulation within microenvironment significantly NPC inflammation. Consequently, simultaneous alleviation of correction acidic anticipated reverse IVDD. Herein, CEPC‐targeted engineered exosomes loaded with salvianolic A incorporated into a CaCO 3 /chitosan hydrogel, forming composite gel, CAP‐sEXOs@Gel. Notably, CAP‐sEXOs@Gel shows long local retention, realizes slow release CAP‐sEXOs specific uptake CEPCs. After CEPCs, reduce intracellular ROS inhibiting hypoxia‐inducible factor‐2α (HIF‐2α)/TfR1 expression. Iron influx inhibition maintenance normal mitochondrial function reduced mtDNA leakage, suppresing pathway. Additionally, component neutralizes H + , thereby alleviating Collectively, this novel hydrogel demonstrates ability concurrently inhibit inflammation, presenting promising therapeutic approach for

Language: Английский

Citations

3