Erroneous Differentiation of Tendon Stem/Progenitor Cells in the Pathogenesis of Tendinopathy: Current Evidence and Future Perspectives DOI

Yucheng Gao,

Hao Wang, Liu Shi

et al.

Stem Cell Reviews and Reports, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

Language: Английский

Cellulose-mediated mechanical property tuning in small intestinal submucosal matrix to enhance stem cell osteogenic differentiation DOI

Boqi Li,

Yufeng Gao, Xiaohu Luo

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139575 - 139575

Published: Jan. 8, 2025

Language: Английский

Citations

0
Epigenetic mechanisms in stem cell therapies for achilles tendinopathy DOI Creative Commons

Zheyang Yuan,

Zheyu Yao,

Xufeng Mao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 13, 2025

Achilles tendinopathy (AT) is a chronic degenerative that affects people’s daily lives. Multiple clinical studies have found current conservative treatments fail to promote quality tendon healing. Recent stem cell therapy can target pathophysiological changes in the by replenishing tendon-derived cells, promoting extracellular matrix (ECM) remodeling, and modulating inflammatory response improve microenvironment of regeneration. And epigenetic modifications play an important role fate determination function. In this review, we provided brief overview biological properties relevant cells. The influence on proliferation, differentiation, immune regulatory function treatment AT was also explored. We focused gene mechanisms controlled DNA methylation, histones non-coding RNAs including microRNAs, circRNAs long RNAs. discuss challenges faced therapies treating their potential solutions. Further research area will provide more comprehensive explanation for AT, leading development stable, safe effective therapies.

Language: Английский

Citations

0
Nanoparticle-Driven Tendon Repair: Role of Vasoactive Intestinal Peptide in Immune Modulation and Stem Cell Enhancement DOI
Hao Wang,

Yucheng Gao,

Jinyu Wang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Tendon repair remains challenging owing to the limited capacity for endogenous repair. Vasoactive intestinal peptide (VIP) promotes bone tissue regeneration; however, its role in tendon unclear. In present study, we demonstrated that VIP stimulated M2 polarization of macrophages and facilitated regeneration by regulating immune homeostasis maintaining function stem/progenitor cells (TSPCs). Additionally, established GelMa-loaded VIP@PLGA@ZIF-8 (VPZ) nanoparticles (VPZG) enable sustained localized release at site patellar injury SD rats. The results vitro experiments VPZG regulated macrophage downregulating NF-κB axis. also promoted efferocytosis suppressed proinflammatory factors. enhanced tenogenic differentiation TSPCs when cocultured with macrophages. vivo, implanted injury, where it released sustainably slowly promote regeneration. This effect was achieved through downregulation expression levels various inflammatory factors, as well regulation local homeostasis. conclusion, our enhancing TSPC function. These findings suggest is a promising avenue clinical improvement treatment.

Language: Английский

Citations

0
Introduction to the Concept of the Secretome in Orthopaedics DOI
Antonio Marmotti,

Martina Coco,

Laura Mangiavini

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Platelet-Rich Plasma-Derived Exosome-Encapsulated Hydrogels Accelerate Diabetic Wound Healing by Inhibiting Fibroblast Ferroptosis DOI

Shanzheng Wang,

Jianyue Wu,

Ke Ren

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Platelet-rich plasma-derived exosomes (PRP-Exos) have recently been considered an optimized strategy for diabetic wound treatment, yet the potential role of PRP-Exos in healing is still unclear. This study aims to investigate mechanisms and utilize hydrogel Pluronic F127 as a carrier maintain sustained release encapsulated PRP-Exos. were isolated from blood healthy individuals characterized, followed by co-culturing with human skin fibroblasts (Diabetes HSF). RNA sequencing (RNA-seq) was used analyze effect on transcriptome normal Diabetes HSF, screening validating crucial mechanism target gene. Then, composed (PRP-Exos/Gel) constructed applied mouse models evaluate mechanism. RNA-seq analysis revealed that significantly upregulated expression FosB HSF. Further intervention HSF showed knocking down induced ferroptosis characterized decreased cell viability, increased oxidative stress, iron ion levels, along downregulation GPX4 SLC7A11 expression, while ACSL4 increased; conversely, overexpression had opposite effect. Subsequently, adding FosB-knocked weakened inhibitory fibroblasts. The synthesized PRP-Exos/Gel exhibited significant thermosensitivity exosomes. In animal experiments, anti-inflammatory effects, evidenced proportion M2 macrophages central granule cells tissue, inhibited fibroblast ferroptosis, thereby accelerating healing. Overall, displays continuous promotes suppressing inflammatory responses which provides new insights methods treatment wounds.

Language: Английский

Citations

0
From conventional to cutting-edge: Exosomes revolutionizing nano-drug delivery systems DOI

Huiyang Fu,

Yinfeng Chen,

Qingyao Fu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 156685 - 156685

Published: Oct. 1, 2024

Language: Английский

Citations

2
Erroneous Differentiation of Tendon Stem/Progenitor Cells in the Pathogenesis of Tendinopathy: Current Evidence and Future Perspectives DOI

Yucheng Gao,

Hao Wang, Liu Shi

et al.

Stem Cell Reviews and Reports, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

Language: Английский

Citations

1