International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(19), P. 10353 - 10353
Published: Sept. 26, 2024
Organ
and
tissue
damage
can
result
from
injury
disease.
How
to
facilitate
regeneration
has
been
a
topic
for
centuries,
still,
we
are
trying
find
agents
use
treatments.
Two
groups
of
biological
substances
known
wound
healing.
Phytochemicals
with
bioactive
properties
form
one
group.
Many
phytochemicals
have
anti-inflammatory
effects
enhance
Recent
studies
described
their
at
the
gene
protein
expression
levels,
highlighting
receptors
signaling
pathways
involved.
The
extremely
large
number
multiple
types
they
activate
suggest
broad
range
applicability
clinical
use.
hydrophobic
nature
many
difficulty
chemical
stabilization
problem.
developments
in
biotechnology
nanotechnology
methods
enabling
researchers
overcome
these
problems.
other
group
is
extracellular
vesicles
(EVs),
which
now
important
functions,
including
improvement
proteins
nanoparticles
contained
mammalian
EVs
as
well
specificity
targets
microRNAs
included
becoming
clear.
Plant-derived
found
contain
phytochemicals.
overlap
wound-healing
capabilities
both
differences
possibility
combinatorial
two
groups,
may
effects.
Stem Cell Research & Therapy,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Feb. 28, 2025
Abstract
Background
Diabetic
foot
ulcers
pose
significant
challenges
for
clinicians
worldwide.
Cell-free
exosome
therapy
holds
great
potential
wound
healing.
Dedifferentiated
fat
cells
(DFATs)
have
been
used
in
tissue
engineering
and
regeneration,
but
there
are
no
reports
on
the
use
of
DFATs-derived
exosomes
diabetic
repair.
Objectives
This
study
aims
to
investigate
whether
DFATs-Exos
accelerated
healing
explore
its
mechanism.
Methods
In
vitro,
were
harvested
from
adipose
treat
endothelial
(ECs)
fibroblasts.
XAV939
was
as
a
Wnt/β-catenin
pathway
inhibitor.
The
biocompatibility
gelatin
methacryloyl
(GelMA)
hydrogel
assessed.
vivo,
DFAT-derived
encapsulated
10%
GelMA
applied
model.
Histological
analysis
closure
rates
evaluated.
Results
promoted
angiogenesis
ECs
significantly
alleviated
high
glucose-induced
inhibition
cell
proliferation
migration
by
activating
pathway.
compared
DFAT-Exos
or
alone,
DFAT-Exos/GelMA
combination
enhanced
collagen
maturity.
Conclusion
animal
model
through
activation
signaling
Journal of Nanobiotechnology,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: March 24, 2025
Exosomes-loaded
hydrogels
have
potential
value
in
wound
treatment.
Current
studies
focus
on
improving
hydrogels'
biocompatibility
and
optimizing
different
stem
cell-derived
exosomes
for
better
therapeutic
effect.
Herein,
we
present
a
novel
biocompatible
recombinant
human
collagen
(RHC)
hydrogel
loading
with
MSCs-derived
promoting
healing.
We
modify
the
RHC
methacrylate
anhydride
(MA)
at
optimal
concentration,
generating
(RHCMA)
ideal
physiochemical
properties
exosome
delivery
(MSC-exos@RHCMA).
Exosomes
derived
from
adipose-derived
MSCs
(ADSC-exos),
bone
marrow-derived
(BMSC-exos)
umbilical
cord
(ucMSC-exos)
are
harvested
culture
supernatants
loaded
into
RHCMA,
respectively.
These
three
systems
exhibit
desired
sustained
release
features,
can
significantly
improve
cell
proliferation
migration.
In
addition,
these
MSC-exos@RHCMAs
show
excellent
performance
treating
wounds
of
rats.
Notably,
demonstrated
that
healing
effect
occurs
best
under
treatment
ucMSC-exos@RHCMA,
following
inflammatory
resolution,
angiogenesis,
formation.
results
would
supply
important
clinical
application
MSC-exos
future.
Journal of Orthopaedic Surgery and Research,
Journal Year:
2025,
Volume and Issue:
20(1)
Published: March 25, 2025
Nonunion
of
fractures
is
a
major
unsolved
problem
in
clinical
treatment
and
prognosis
orthopedics.
Bone
marrow
mesenchymal
stem
cell
(BMSC)
exosomes
have
been
proven
to
be
involved
mediating
tissue
bone
regeneration
variety
diseases.
However,
the
role
BMSC
fracture
nonunion
unclear.
were
injected
into
rat
model
fracture,
fracture-healing
site
was
detected
by
micro-CT
serum
metabolites
analyzed
LC-MS/MS.
The
results
showed
that
could
successfully
isolated
from
BMSCs
cultured
an
exosome-free
medium.
Compared
with
group,
exosome-treated
rats
healing
obviously.
PBS
there
158
up-regulated
differential
abundance
(DAMs)
79
down-regulated
DAMs
BMSC-exo
group.
enriched
'Th1
Th2
differentiation',
'ErbB
signaling
pathway',
'PPAR
pathway'
'HIF-1
related
function
proliferation
differentiation.
DAMs-PE
HIF-1
pathway
metabolite
promote
healing.
Our
study
reveals
mechanism
which
BMSC-exosome
improves
process
through
metabolic
reprogramming
provides
reference
for
nonunion.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(19), P. 10353 - 10353
Published: Sept. 26, 2024
Organ
and
tissue
damage
can
result
from
injury
disease.
How
to
facilitate
regeneration
has
been
a
topic
for
centuries,
still,
we
are
trying
find
agents
use
treatments.
Two
groups
of
biological
substances
known
wound
healing.
Phytochemicals
with
bioactive
properties
form
one
group.
Many
phytochemicals
have
anti-inflammatory
effects
enhance
Recent
studies
described
their
at
the
gene
protein
expression
levels,
highlighting
receptors
signaling
pathways
involved.
The
extremely
large
number
multiple
types
they
activate
suggest
broad
range
applicability
clinical
use.
hydrophobic
nature
many
difficulty
chemical
stabilization
problem.
developments
in
biotechnology
nanotechnology
methods
enabling
researchers
overcome
these
problems.
other
group
is
extracellular
vesicles
(EVs),
which
now
important
functions,
including
improvement
proteins
nanoparticles
contained
mammalian
EVs
as
well
specificity
targets
microRNAs
included
becoming
clear.
Plant-derived
found
contain
phytochemicals.
overlap
wound-healing
capabilities
both
differences
possibility
combinatorial
two
groups,
may
effects.
