Size Effect-based Improved Antioxidant Activity of Selenium Nanoparticles Regulating Anti-PI3K-mTOR and Ras-MEK Pathways for Treating Spinal Cord Injury to Avoid Hormone Shock-induced Immunosuppression DOI Creative Commons
Peixin Liu, Xiaodong Liu,

Zihao Wu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Abstract Spinal cord injury (SCI) is a critical condition affecting the central nervous system that often has permanent and debilitating consequences, including secondary injuries. Oxidative damage inflammation are factors in pathological processes. Selenium nanoparticles have demonstrated significant antioxidative anti-inflammatory properties via non-immunosuppressive pathway; however, their clinical application been limited by inadequate stability functionality to cross blood-spinal barrier (BSCB). This study proposed synthesis method for ultra-small-diameter lentinan Se (LNT-UsSeNPs) with significantly superior reactive oxygen species (ROS) scavenging capabilities compared conventional (LNT-SeNPs). These compounds effectively protected PC-12 cells from oxidative stress-induced cytotoxicity, alleviated mitochondrial dysfunction, reduced apoptosis. In vivo studies indicated LNT-UsSeNPs efficiently penetrated BSCB inhibited apoptosis of spinal neurons. Ultimately, directly regulated PI3K-AKT-mTOR Ras-Raf-MEK-ERK signaling pathways regulating selenoproteins achieve therapy. Owing ultra-small size, exhibited strong penetration potent effects without compromising immune function. findings suggest promising candidates further development nanomedicine effective treatment SCI.

Language: Английский

Size effect-based improved antioxidant activity of selenium nanoparticles regulating Anti-PI3K-mTOR and Ras-MEK pathways for treating spinal cord injury to avoid hormone shock-induced immunosuppression DOI Creative Commons
Peixin Liu, Xiaodong Liu,

Zihao Wu

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 16, 2025

Language: Английский

Citations

2
β2 integrin regulates neutrophil trans endothelial migration following traumatic brain injury DOI Creative Commons
Lei Li,

Ruilong Peng,

Cong Wang

et al.

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 8, 2025

Neutrophils are the first responders among peripheral immune cells to infiltrate central nervous system following a traumatic brain injury (TBI), triggering neuroinflammation that can exacerbate secondary tissue damage. The precise molecular controls dictate inflammatory behavior of neutrophils post-TBI, however, remain largely elusive. Our comprehensive analysis landscape surrounding trauma in TBI mice has revealed significant alteration abundance β2 integrin (ITGB2), predominantly expressed by and closely associated with responses. Using fluid percussion (FPI) mouse model, we investigated therapeutic efficacy Rovelizumab, an agent blocks ITGB2. treatment demonstrated improvements neurologic function mice, attenuating blood–brain barrier permeability, mitigating oxidative stress mediator release, enhancing cerebral perfusion. Moreover, ITGB2 blockade effectively limited adherence, migration, infiltration neutrophils, impeded formation neutrophil extracellular traps (NETs) upon their activation. Finally, it was mediates these effects mainly through its interaction intercellular adhesion molecule-1 (ICAM 1) endotheliocyte. These findings collectively illuminate as crucial switch governs adverse post-TBI could be targeted improve clinical outcome patients.

Language: Английский

Citations

1
Brain-targeted M2 macrophage membrane-hybrid biomimetic liposomes for treatment of traumatic brain injury DOI
Yizhi Zhang,

Jintao Shen,

Junzhe Yang

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 125316 - 125316

Published: Feb. 1, 2025

Language: Английский

Citations

0
Exosome‐Functionalized Self‐Carrier Enzyme‐Like/Drug With Triple Amplified Anti‐Oxidative Stress for Synergistic Depression Therapy DOI
Hanqing Wang,

Yunzhu Xu,

Zicheng Zhang

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Depression, a severe disorder affecting both physical and mental health, is commonly treated with first‐line antidepressants, which often exhibit limited efficacy due to poor penetration of the blood‐brain barrier (BBB) significant side effects, thus requiring exploitation biocompatible effective treatments. Recent studies suggest that depression closely linked an imbalance in oxidative stress subsequent inflammatory responses. Antioxidant therapies targeting are therefore emerging as promising strategies. In this study, exosome‐functionalized geniposide (GEN) self‐carried Prussian blue (PB) nanotherapeutic approach fabricated realize efficient BBB for synergistic therapy. The porous PB carrier possesses multi‐enzyme capabilities, can effectively scavenge accumulated ROS, protecting slightly acidic environment released GEN from oxidation, subsequently works simultaneously activate Nrf2‐ARE pathway, enhancing body's defense mechanisms synergistically. triple‐amplified anti‐oxidant strategy nanomaterial shown mitigate microglial activation reduction neuroplasticity, ultimately alleviating pathological markers depression. Overall, constructed nanomaterials underscore therapeutic potential anti‐oxidative removal ROS pathway treatment

Language: Английский

Citations

0
Size Effect-based Improved Antioxidant Activity of Selenium Nanoparticles Regulating Anti-PI3K-mTOR and Ras-MEK Pathways for Treating Spinal Cord Injury to Avoid Hormone Shock-induced Immunosuppression DOI Creative Commons
Peixin Liu, Xiaodong Liu,

Zihao Wu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Abstract Spinal cord injury (SCI) is a critical condition affecting the central nervous system that often has permanent and debilitating consequences, including secondary injuries. Oxidative damage inflammation are factors in pathological processes. Selenium nanoparticles have demonstrated significant antioxidative anti-inflammatory properties via non-immunosuppressive pathway; however, their clinical application been limited by inadequate stability functionality to cross blood-spinal barrier (BSCB). This study proposed synthesis method for ultra-small-diameter lentinan Se (LNT-UsSeNPs) with significantly superior reactive oxygen species (ROS) scavenging capabilities compared conventional (LNT-SeNPs). These compounds effectively protected PC-12 cells from oxidative stress-induced cytotoxicity, alleviated mitochondrial dysfunction, reduced apoptosis. In vivo studies indicated LNT-UsSeNPs efficiently penetrated BSCB inhibited apoptosis of spinal neurons. Ultimately, directly regulated PI3K-AKT-mTOR Ras-Raf-MEK-ERK signaling pathways regulating selenoproteins achieve therapy. Owing ultra-small size, exhibited strong penetration potent effects without compromising immune function. findings suggest promising candidates further development nanomedicine effective treatment SCI.

Language: Английский

Citations

0