A Smart MMP‐9‐responsive Hydrogel Releasing M2 Macrophage‐derived Exosomes for Diabetic Wound Healing

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

Chronic diabetic wounds are characterized by prolonged inflammation and excessive accumulation of M1 macrophages, which impede the healing process. Therefore, resolving promptly transitioning to proliferative phase critical steps for effective wound healing. Exosomes have emerged as a promising therapeutic strategy. In this study, smart hydrogel capable responding pathological cues in inflammatory microenvironment promote transition from proliferation delivering M2 macrophage-derived exosomes (M2-Exos) is developed. The synthesized through cross-linking oxidized dextran, matrix metalloproteinase (MMP)-9-sensitive peptide, carboxymethyl chitosan containing M2-Exos. response elevated MMP-9 concentrations microenvironment, demonstrates diagnostic logic, adjusting release kinetics M2-Exos accordingly. on-demand facilitated macrophage polarization phenotype, thereby promoting accelerating transcriptomic analysis further reveals that MMP-9-responsive with delivery exerts anti-inflammatory regenerative effects downregulating inflammation-related pathways. This study introduces an innovative, microenvironment-responsive exosome system enables precise control agent release, offering personalized approach treatment chronic wounds.

Language: Английский

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Selenium nanoparticles activate selenoproteins to mitigate septic lung injury through miR-20b-mediated RORγt/STAT3/Th17 axis inhibition and enhanced mitochondrial transfer in BMSCs

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 20, 2025

Sepsis-induced acute lung injury (ALI) remains a critical clinical challenge with complex inflammatory pathogenesis. While bone marrow mesenchymal stem cells (BMSCs) demonstrate therapeutic potential through anti-inflammatory and cytoprotective effects, their age-related functional decline limits utility. This study developed chitosan-functionalized selenium nanoparticles (SeNPs@CS, 100 nm) to rejuvenate BMSCs miR-20b-mediated selenoprotein biosynthesis. Mechanistic investigations revealed that SeNPs@CS-treated exhibited enhanced mitochondrial transfer capacity, delivering mitochondria damaged alveolar epithelial (AECII) for cellular repair. Concurrently, miR-20b upregulation suppressed the RORγt/STAT3/Th17 axis, reducing pro-inflammatory Th17 cell differentiation in CD4+ T lymphocytes. The dual-target mechanism integrates immunomodulation via pathway inhibition rejuvenation therapy, representing paradigm-shifting approach ALI management. These engineered mitigated markers murine models, demonstrating superior efficacy conventional BMSC therapies. Our findings establish SeNPs@CS-modified as novel platform combining nanotechnology-enhanced engineering precision immunometabolic regulation, providing new avenues treatment of sepsis-induced ALI.

Language: Английский

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Genome-Wide Identification of the BTB Domain-Containing Protein Gene Family in Pepper (Capsicum annuum L.)

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3429 - 3429

Published: April 6, 2025

Pepper (Capsicum annuum L.), recognized as a globally preeminent vegetable, holds substantial economic and nutritional value. The BTB (broad-complex, tramtrack, bric-a-brac) family of proteins, characterized by highly conserved domain, also denoted the POZ are intricately involved in diverse array biological processes. However, existing corpus research regarding pepper genes remains relatively meager. In this study, total 72 CaBTB gene members were meticulously identified from entire genome pepper. Phylogenetic analysis illuminated presence conspicuous collinear relationships between those its closely affiliated species. Gene expression profiling RT-qPCR revealed that multiple exhibited pronounced differential under treatment regimens. Expression pattern unveiled CaBTB25 manifested remarkably elevated abundance leaves. Moreover, promoters replete with an light-responsive cis-elements. Our comprehensive in-depth explorations into subcellular localization was predominantly detected to localize within nucleus lacked transcriptional activation. This provides crucial theoretical edifice, enabling more profound understanding functions pepper, thereby underscoring potential significance intricate network gene-environment interactions.

Language: Английский

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Pre-vascularized Bioactive Scaffold Driven by Bidirectional Cellular Communication Enhances Bone Repair

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 253, P. 114741 - 114741

Published: April 28, 2025

Language: Английский

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