Hormonal and Allosteric Regulation of the Luteinizing Hormone/Chorionic Gonadotropin Receptor DOI Creative Commons
А. О. Шпаков

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(9)

Published: Aug. 30, 2024

Luteinizing hormone (LH) and human chorionic gonadotropin (CG), like follicle-stimulating hormone, are the most important regulators of reproductive system. They exert their effect on cell through LH/CG receptor (LHCGR), which belongs to family G protein-coupled receptors. Binding induces interaction LHCGR with various types heterotrimeric proteins (Gs, Gq/11, Gi) β-arrestins, leads stimulation (Gs) or inhibition (Gi) cyclic adenosine monophosphate-dependent cascades, activation phospholipase pathway (Gq/11), also formation signalosomes that mediate mitogen-activated protein kinases (β-arrestins). The efficiency selectivity intracellular cascades by different gonadotropins varies, is due differences in ligand-binding site LHCGR. Gonadotropin signaling largely depends status N- O-glycosylation LH CG, homo- heterodimeric complexes, cell-specific microenvironment presence autoantibodies it, allosteric mechanisms implementation these influences, multiplicity sites loci development low-molecular-weight profiles pharmacological activity, can be used medicine for correction disorders assisted technologies, promising. These other issues regarding hormonal regulation summarized discussed this review.

Language: Английский

Primary ovarian insufficiency: update on clinical and genetic findings DOI Creative Commons
Silvia Federici, Raffaella Rossetti, Silvia Moleri

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 26, 2024

Primary ovarian insufficiency (POI) is a disorder of insufficient follicle function before the age 40 years with an estimated prevalence 3.7% worldwide. Its relevance emerging due to increasing number women desiring conception late or beyond third decade their lives. POI clinical presentation extremely heterogeneous possible exordium as primary amenorrhea dysgenesis secondary different congenital acquired abnormalities. significantly impacts non only on fertility prospect affected but also general, psychological, sexual quality life, and, furthermore, long-term bone, cardiovascular, and cognitive health. In several cases underlying cause remains unknown thus, these forms are still classified idiopathic. However, we now know menopause inheritable trait has strong genetic background. This confirmed by existence candidate genes, experimental natural models. The most common contributors X chromosome-linked defects. Moreover, variable expressivity defect suggests it can be considered multifactorial oligogenic defect. Here, present updated review findings principal X-linked autosomal genes involved in syndromic non-syndromic POI. We provide current information management premature hypoestrogenic state well preservation subjects at risk

Language: Английский

Citations

7
Histone Lactylation Is Involved in Mouse Oocyte Maturation and Embryo Development DOI Open Access
Diqi Yang, Haoyi Zheng,

Wenjie Lu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4821 - 4821

Published: April 28, 2024

Numerous post-translational modifications are involved in oocyte maturation and embryo development. Recently, lactylation has emerged as a novel epigenetic modification implicated the regulation of diverse cellular processes. However, it remains unclear whether occurs during development Herein, lysine (Kla) were determined mouse early by immunofluorescence staining. Exogenous lactate was supplemented to explore consequences modulating histone levels on processes transcriptomics. Results demonstrated that lactylated proteins widely present mice with tissue- cell-specific distribution. During maturation, for H3K9la, H3K14la, H4K8la, H4K12la most intense at germinal vesicle (GV) stage subsequently weakened or disappeared. Further, supplementing culture medium 10 mM sodium elevated both rate Kla GV oocytes, there substantial increases metaphase II (MII) oocytes. It altered transcription molecules oxidative phosphorylation. Moreover, changed dynamically embryogenesis. Sodium enhanced significantly increased lactylation, while impacting glycolytic gene transcription. This study reveals roles development, providing new insights improving quality.

Language: Английский

Citations

5
Non-Male Factor Only—ICSI Can Overcome Oocyte Factor in PCOS Patients DOI Open Access

Yasmin Shibli Abu Raya,

Nardin Aslih, Yuval Atzmon

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(1), P. 244 - 244

Published: Jan. 3, 2025

Background: In this research, we retrospectively studied the influence of IVF vs. ICSI technique on embryo morphokinetics by means a time-lapse incubator in fresh cycles. Methods: A total 2645 treatment cycles resulting ovum pick-up 11,471 fertilized oocytes were included research from 2018 to 2022. The embryos grouped according or ICSI. Embryonic development was monitored using incubator, and they transferred day 3 5. Results: group developed faster had less fragmentation. However, fewer 2PNs achieved more discarded compared IVF. When sibling treated with either analyzed, found that resulted quicker higher KIDScores. Discussion: anovulation PCOS subgroups primary contributors high KIDScores oocytes, indicating might have beneficial effects oocyte factors, similar positive results it provides when male factors are involved. Conclusions: Women undergoing better used spontaneous This study reveals is superior female factor infertility.

Language: Английский

Citations

0
Regulation of cell cycle in plant gametes: when is the right time to divide? DOI Creative Commons
Sara Simonini

Development, Journal Year: 2025, Volume and Issue: 152(2)

Published: Jan. 15, 2025

ABSTRACT Cell division is a fundamental process shared across diverse life forms, from yeast to humans and plants. Multicellular organisms reproduce through the formation of specialized types cells, gametes, which at maturity enter quiescent state that can last decades. At point fertilization, signalling lifts triggers cell cycle reactivation. Studying how regulated during plant gamete development fertilization challenging, decades research have provided valuable, yet sometimes contradictory, insights. This Review summarizes current understanding regulation, development, quiescence, fertilization-triggered

Language: Английский

Citations

0
Correlation between follicle diameter and pre-ovulatory metabolic profile in Bos grunniens DOI Creative Commons
Jingbin Yan,

Yuxin Fu,

Lan Lan

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 28, 2025

Introduction In this study, we investigated the metabolic profiles of yak ( Bos grunniens ) follicles during development period from perspective metabolomics, aiming to screen out differential metabolites in different sizes and potential pathways follicle provide a basis for study developmental mechanisms further stage development. Methods A total 20 four-year-old female yaks were selected, collected after slaughter divided into d < 3 mm, 3–6 mm > 6 mm. The follicular fluid was collected, replicates per group subjected LC–MS assay, combined with multidimensional unidimensional statistical analyses between sizes. Differential enriched KEGG that might be related Results We found most mainly amino acid metabolism pathways, energy other cofactor synthesis, small large follicle, 2-Lysophosphatidylcholine, PC (17:0/0:0), (16:0/0:0), LysoPC (18:0/0:0) down-regulated; Dioctyl succinate, P-Coumaraldehyde, ISOPRENE, L-Isoleucine, succinate up-regulated. Conclusion These results suggest metabolism, production steroid hormones their metabolites, activity granulosa cells play important roles theoretical exploration yak.

Language: Английский

Citations

0
Editing the growth differentiation factor 9 gene affects porcine oocytes in vitro maturation by inactivating the maturation promoting factor DOI

Yafei Jiao,

Alian Liao,

X. S. Jiang

et al.

Theriogenology, Journal Year: 2025, Volume and Issue: 236, P. 120 - 136

Published: Feb. 7, 2025

Language: Английский

Citations

0
Neddylation Inhibition Affects Cell Proliferation and Steroidogenesis in Sheep Follicular Granulosa Cells DOI
Lihua Lyu, Xiaowei Qin,

Haoyu Xiu

et al.

Theriogenology, Journal Year: 2025, Volume and Issue: 237, P. 99 - 109

Published: Feb. 18, 2025

Language: Английский

Citations

0
Decoding the Molecular Landscape of Prepubertal Oocyte Maturation: GTPBP4 as a Key Driver of In Vitro Developmental Competence DOI Creative Commons
Jianpeng Qin,

Yaozong Wei,

Ao Ning

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

ABSTRACT The intricate mechanisms driving oocyte maturation remain only partially understood, especially within the domains of domestic animal reproduction and translational medicine. In case prepubertal girls, clinical challenge is pronounced, as ovarian tissue cryopreservation‐though promising‐remains an experimental technique necessitating rigorous scientific validation to guarantee developmental potential preserved materials facilitate broader adoption. To address these knowledge gaps, while considering ethical implications, we applied transcriptome translatome sequencing comprehensively profile transcriptional dynamics in adult goats. Our analyses uncovered a sequential transition gene expression regulation, shifting from cytoplasmic processes chromosome segregation during process. Comparative profiling between goat oocytes revealed critical regulatory factors essential for maturation. These include genes involved organelle function ( GTPBP4 TOMM7 ), spindle organisation CKS2 , CCP110 CKAP5 ESCO1 ) CENPE CENPF CENPN SGO2 ). Functional through vitro experiments demonstrated that significantly enhances competence oocytes. This enhancement occurs promote cell cycle progression, mRNA translation. findings provide detailed map molecular events underpinning offer new perspectives on strategies required females. Translating insights humans, this research highlights fertility preservation such cryopreservation transplantation, follicle culture, meiotic artificial ovary technologies. Moreover, identified have significant implications improving reproductive efficiency breeding, bridging basic science.

Language: Английский

Citations

0
Intergenerationzal genetic instability and anticipation in spinocerebellar ataxia type 3: The impact of parental gender DOI

Mao‐Lin Cui,

Hao‐Ling Xu,

Gui‐He Li

et al.

Gene Reports, Journal Year: 2025, Volume and Issue: unknown, P. 102206 - 102206

Published: March 1, 2025

Language: Английский

Citations

0
MPF Regulates Oocyte and Embryo Development During Parthenogenesis Induction in Silkworm, Bombyx mori DOI Creative Commons
Chao Ma, Fang Xu,

Chengjie Hu

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 361 - 361

Published: March 31, 2025

In most species, oocytes are arrested at the prophase or metaphase of meiosis I and require sperm-derived external stimuli to resume meiosis. Maturation-promoting factor (MPF) is an oocyte maturation composing catalytic subunit Cdc2 regulatory CycB that can restart stalled this study, we demonstrated MPF activity affected parthenogenesis induction in model lepidopteran insect Bombyx mori using activator inhibitor interference. We found upregulation significantly increased rate, whereas downregulation reduced it. Furthermore, inhibition also led a delay embryonic development. Given its evolutionary conservation, emerges as potential universal target for manipulating reproductive outcomes, offering broad applications genetics selective breeding.

Language: Английский

Citations

0