Diet-Induced Obesity Alters Granulosa Cell Transcriptome and Ovarian Immune Environment in Mice

Life, Journal Year: 2025, Volume and Issue: 15(3), P. 330 - 330

Published: Feb. 20, 2025

Obesity affects female reproductive performance by impairing the ovarian and uterine environments. Using a diet-induced obesity mouse model, we examined whether high-fat diet (HFD) regimen gene expression profile in granulosa cells (GCs) short-term HFD has similar effects on as long-term HFD. C57BL/6J mice were fed or normal (ND) for 16–18 weeks (long-term group) 4 (short-term group). GCs collected from each group of RNA-sequencing. RT-PCR immunofluorescence staining performed to validate results. RNA-sequencing analyses revealed that several immediate early genes, including growth response 1 (Egr1), an important mediator ovulation, significantly downregulated GCs. Protein tyrosine phosphatase receptor type C (Ptprc) hematopoietic prostaglandin D synthase (Hpgds), both which are associated with increased inflammation, upregulated Downregulation Egr1 was also confirmed mice, suggesting it constitutes change Increased transcription factors suggests may affect overall transcriptional landscape. The results indicate possible modulation immune environment ovaries. These provide novel insights into molecular changes obese

Language: Английский

Comprehensive single-cell transcriptome analysis of autologous platelet-rich plasma therapy on human thin endometrium

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 26, 2025

Therapeutics for thin endometrium (TE) have emerged, with autologous platelet-rich plasma (PRP) therapy gaining significant attention. In the present study, ten eligible TE patients were recruited PRP infusion. Endometrial tissue biopsies collected before and after (paired samples) subjected to single-cell RNA sequencing (scRNA-seq). Additionally, haematoxylin eosin (HE) immunohistochemistry (IHC) employed validate changes in protein markers. The results demonstrated increased average endometrial thickness these patients. Cellular trajectory reconstruction analysis using gene counts expression (CytoTRACE) scores indicated that high-stemness cells more enriched proliferating stromal (pStr) or (Str) post-PRP samples, while greater stemness was observed glandular epithelial (GE) luminal (LE). Gene set variation (GSVA) revealed differences mesenchymal‒epithelial transition (MET)-related signature between paired samples. Furthermore, an number of macrophages, particularly M1-type detected As first study investigate effects via transcriptomic analysis, our findings suggest may enhance high-stemness, stimulate MET, boost macrophage function. These insights contribute a better understanding mechanisms underlying its potential treating

Language: Английский

Restoration of vascular dysfunction resulting from maternal high-fat diet via modulation of the NLRP3/IL-1β axis

Clinical and Experimental Hypertension, Journal Year: 2024, Volume and Issue: 47(1)

Published: Dec. 26, 2024

This study investigated the impact of maternal high-fat diet on vascular function and endothelial homeostasis in offspring. We found that offspring exposed to exhibited elevated blood pressure, impaired abdominal aortic function, imbalance. These changes were accompanied by increased levels reactive oxygen species (ROS) upregulation pro-inflammatory cytokines (including IL-1β, TNF-α, IL-6, IL-10). Treatment with NLRP3 or IL-1β inhibitors prevented deterioration reduced NO production, inflammation induced exposure compared control group. The findings suggest during pregnancy, mitigating impairments can be achieved inhibiting NLRP3/IL-1β pathway.

Language: Английский