Differential Activity and Expression of Proteasome in Seminiferous Epithelium During Mouse Spermatogenesis

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 494 - 494

Published: Jan. 9, 2025

Proteasome-mediated protein degradation is essential for maintaining cellular homeostasis, particularly during spermatogenesis, where extensive transformations, such as spermatid differentiation, require precise turnover. A key player in this process the ubiquitin-proteasome system (UPS). This study aimed to investigate proteasome enzymatic activity at different stages of spermatogenic cycle within seminiferous tubules mice and explore regulatory mechanisms that influence its proteolytic function. Specifically, we assessed trypsin-like, chymotrypsin-like, peptidyl-glutamyl-peptide-hydrolyzing (PGPH) activities proteasome. Additionally, examined expression catalytic structural subunits 20S core, assembly core with complexes, phosphorylation status various segments tubules. Our findings demonstrated distinct patterns proteasomal analyzed segments. While levels remained consistent, significant differences were detected mediated by kinase A. These results indicate finely regulated through multiple depending on specific stage epithelial cycle, highlighting complexity proteostasis spermatogenesis.

Language: Английский

Genome-wide exploration of soybean domestication traits: integrating association mapping and SNP × SNP interaction analyses

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Soybean domestication has been essential for crop evolution, adaptation, and modern breeding. Despite advancements in understanding soybean genetics, the genetic basis of DRTs yet to be fully explored, particularly context genome-wide association studies (GWASs) gene interaction analyses (epistasis). This study evaluated 198 diverse accessions using 23,574 high-quality SNPs obtained via ddRAD-seq. Nine key DRTs—including those related seed size (length, width, thickness), coat color, cotyledon hypocotyl stem growth habit, flower pod pubescence, pod-shattering—were phenotyped two environments. A GWAS conducted FarmCPU BLINK models identified 78 significant SNPs, 14 consistently detected across both environments models, demonstrating stability. Notably, SNP rs.Gm16.29778273 linked pod-shattering resistance. The functional annotation three known quantitative trait loci (QTLs)/genes revealed 11 novel candidate genes associated with DRTs, providing insights into their roles Gene Ontology (GO) terms. main effect × analysis that rs.Gm13.16695800 exhibits a pleiotropic effect, controlling color. Furthermore, 324 epistatic interactions were identified, influencing expression thereby highlighting complex architecture underlying these traits. These findings offer valuable traits higher yield. They provide solid foundation developing marker-assisted selection (MAS) strategies improve breeding resilient, high-yielding varieties.

Language: Английский

Genome-wide exploration of soybean domestication traits: integrating association mapping and SNP × SNP interaction analyses

Plant Molecular Biology, Journal Year: 2025, Volume and Issue: 115(2)

Published: April 1, 2025

Language: Английский