Development of Free Testosterone Chemiluminescence Detection Kit and Its Clinical Application DOI
Shuang Han, Min Liu, Simin Zhao

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Abstract Background: Free testosterone makes up only 1–2% of the total in blood, but it is active form and enters cells directly to work. The level substantially affected by sex hormone-binding globulin, whereas free can better reflect actual biological activity. clinical detection levels serum important for diagnosis related diseases, such as male hypogonadism, polycystic ovary syndrome, metabolic osteoporosis, Alzheimer's disease, hypogonadism. However, existing methods have problems, insufficient sensitivity, complex operation, high cost, there an urgent need develop efficient accurate technologies. We aimed a chemiluminescence kit conducted comprehensive performance evaluation. Methods: content was quantified using competitive immunoassay method. Free derivatives competed binding biotinylated testosterone-specific antibodies. reaction procedure determined concentration magnetic beads optimized, antibodies were biotinylated, labeled with acridinium ester, working concentrations both fine-tuned. Following determination process, assay evaluated through series tests assessing linearity, limit blank, accuracy, precision, stability, specificity, relevance. Samples collected from 1615 2035 female patients, their measured. Finally, significance diagnostic applications analyzed. Results: The linear r > 0.99, blank 0.021 pg/mL, accuracy deviation < 5%, imprecision stability lasted 12 months. There no cross-reactivity similar substances. In total, 392 samples analysis methodological comparison conducted. correlation coefficient 0.9941, meeting requirements. patient detection. showed significant differences among patients different diagnoses. Prostate malignancy associated lower levels, other diagnoses (such those hair loss) higher levels. Therefore, could serve reference indicator assessment certain diseases. Conclusion: developed this study exhibited excellent performance, low degree automation. It fully meets requirements When tested on samples, be regarded which help doctors make treatments

Language: Английский

The use of deidentified organ donor testes for research DOI Creative Commons
Marina V. Pryzhkova, Marnie W. Skinner, Juliana I. Candelaria

et al.

Andrology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Our knowledge of testis development and function mainly comes from research using mammalian model organisms, primarily the mouse. However, there are integral differences between men other species regarding cellular composition expression profiles during fetal post‐natal in mature testis. Therefore, to specifically learn more about human function, is a need use tissue for research. Human testicular tissues that have been donated allowed extensive molecular cytological assessments, as well single‐cell transcriptome epigenome analyses. These also used cell technologies vitro models aim improve infertility treatments diagnostics. Biopsied material taken patients designated usually very small size unsuitable comprehensive studies. On hand, whole testes obtained deceased, deidentified donors has become valuable resource assess conservation humans organisms identify human‐specific phenomena. This review discusses acquisition their basic science

Language: Английский

Citations

0
Development of Free Testosterone Chemiluminescence Detection Kit and Its Clinical Application DOI
Shuang Han, Min Liu, Simin Zhao

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Abstract Background: Free testosterone makes up only 1–2% of the total in blood, but it is active form and enters cells directly to work. The level substantially affected by sex hormone-binding globulin, whereas free can better reflect actual biological activity. clinical detection levels serum important for diagnosis related diseases, such as male hypogonadism, polycystic ovary syndrome, metabolic osteoporosis, Alzheimer's disease, hypogonadism. However, existing methods have problems, insufficient sensitivity, complex operation, high cost, there an urgent need develop efficient accurate technologies. We aimed a chemiluminescence kit conducted comprehensive performance evaluation. Methods: content was quantified using competitive immunoassay method. Free derivatives competed binding biotinylated testosterone-specific antibodies. reaction procedure determined concentration magnetic beads optimized, antibodies were biotinylated, labeled with acridinium ester, working concentrations both fine-tuned. Following determination process, assay evaluated through series tests assessing linearity, limit blank, accuracy, precision, stability, specificity, relevance. Samples collected from 1615 2035 female patients, their measured. Finally, significance diagnostic applications analyzed. Results: The linear r > 0.99, blank 0.021 pg/mL, accuracy deviation < 5%, imprecision stability lasted 12 months. There no cross-reactivity similar substances. In total, 392 samples analysis methodological comparison conducted. correlation coefficient 0.9941, meeting requirements. patient detection. showed significant differences among patients different diagnoses. Prostate malignancy associated lower levels, other diagnoses (such those hair loss) higher levels. Therefore, could serve reference indicator assessment certain diseases. Conclusion: developed this study exhibited excellent performance, low degree automation. It fully meets requirements When tested on samples, be regarded which help doctors make treatments

Language: Английский

Citations

0