Physiological and pathological consequences of exosomes at the blood–brain-barrier interface DOI Creative Commons

Leila Salimi,

Fatemehsadat Seyedaghamiri,

Mohammad Karimipour

et al.

Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 21(1)

Published: May 19, 2023

Abstract Blood–brain barrier (BBB) interface with multicellular structure controls strictly the entry of varied circulating macromolecules from blood-facing surface into brain parenchyma. Under several pathological conditions within central nervous system, integrity BBB is disrupted due to abnormal crosstalk between cellular constituents and recruitment inflammatory cells. Exosomes (Exos) are nano-sized extracellular vesicles diverse therapeutic outcomes. These particles transfer a plethora signaling molecules potential modulate target cell behavior in paracrine manner. Here, current review article, properties Exos their alleviation compromised were discussed.

Language: Английский

Pericytes, inflammation, and diabetic retinopathy DOI
Benjamin G. Spencer, Jose J. Estevez, Ebony Liu

et al.

Inflammopharmacology, Journal Year: 2019, Volume and Issue: 28(3), P. 697 - 709

Published: Oct. 14, 2019

Language: Английский

Citations

88
The Role of Neurovascular System in Neurodegenerative Diseases DOI
Ajmal Ahmad, Vanisha Patel, Jianfeng Xiao

et al.

Molecular Neurobiology, Journal Year: 2020, Volume and Issue: 57(11), P. 4373 - 4393

Published: July 28, 2020

Language: Английский

Citations

74
Microglia and the Blood–Brain Barrier: An External Player in Acute and Chronic Neuroinflammatory Conditions DOI Open Access
Giorgia Serena Gullotta, Giuseppe Costantino, Maria Angela Sortino

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9144 - 9144

Published: May 23, 2023

Microglia are the resident immune cells of central nervous system that guarantee surveillance and exert also a modulating role on neuronal synaptic development function. Upon injury, microglia get activated modify their morphology acquiring an ameboid phenotype pro- or anti-inflammatory features. The active in blood–brain barrier (BBB) function interaction with different cellular components BBB—endothelial cells, astrocytes pericytes—are described. Here, we report specific crosstalk all BBB cell types focusing particular involvement modulation neuroinflammatory conditions occur conjunction acute event, such as stroke, slow neurodegenerative disease, Alzheimer’s disease. potential to dual role, either protective detrimental, depending disease stages environmental conditioning factors is discussed.

Language: Английский

Citations

32
Unveiling the impact of aging on BBB and Alzheimer's disease: Factors and therapeutic implications DOI
Niraj Kumar Jha, Niraj Kumar Jha, Mohana Vamsi Nuli

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 98, P. 102224 - 102224

Published: Feb. 10, 2024

Language: Английский

Citations

14
Angiogenesis within Atherosclerotic Plaques: Mechanical Regulation, Molecular Mechanism and Clinical Diagnosis DOI Creative Commons
Hanxiao Chen,

Chih-Yu Peng,

Fei Fang

et al.

Mechanobiology in Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 100114 - 100114

Published: Feb. 1, 2025

Language: Английский

Citations

1
Serum biomarkers of neuroinflammation and blood-brain barrier leakage in amyotrophic lateral sclerosis DOI Creative Commons
Maize C. Cao, Erin E. Cawston, Grace Chen

et al.

BMC Neurology, Journal Year: 2022, Volume and Issue: 22(1)

Published: June 11, 2022

Amyotrophic lateral sclerosis (ALS) is an incurable and rapidly progressive neurological disorder. Biomarkers are critical to understanding disease causation, monitoring progression assessing the efficacy of treatments. However, robust peripheral biomarkers yet be identified. Neuroinflammation breakdown blood-brain barrier (BBB) common familial sporadic ALS may produce a unique biomarker signature in blood. Using cytometric bead array (n = 15 participants per group (ALS or control)) proteome profiling 6 control)), we assessed total 106 serum cytokines, growth factors, BBB markers control participants. Further, primary human brain pericytes, which maintain BBB, were used as biosensor inflammation following pre-treatment with serum. Principal components analysis all profile data showed no clustering sera, individual proteins met threshold for statistical difference between controls (adjusted P values). 20 most changed sera medium effect size (Cohen's d 0.67) cluster their levels together identified three sample subsets; control-only, mixed control-ALS, ALS-only. These predominantly pro-angiogenic including fractalkine, BDNF, EGF, PDGF, Dkk-1, MIF angiopoietin-2. S100β, protein highly concentrated glial cells therefore marker leakage when found blood, was unchanged serum, suggesting that profiles reflective rather than CNS biofluids. Finally, pericytes remained proliferative secretome by chronic exposure Our exploratory study suggests cytokine not small studies ALS, but larger using multiplexed factors identify pathogenesis.

Language: Английский

Citations

37
Effects of choline containing phospholipids on the neurovascular unit: A review DOI Creative Commons
Proshanta Roy, Daniele Tomassoni, Giulio Nittari

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: Sept. 23, 2022

The roles of choline and choline-containing phospholipids (CCPLs) on the maintenance progress neurovascular unit (NVU) integrity are analyzed. NVU is composed neurons, glial vascular cells ensuring correct homeostasis blood-brain barrier (BBB) indirectly function central nervous system. CCPLs phosphatidylcholine (lecithin), cytidine 5′-diphosphocholine (CDP-choline), alphoscerate or α-glyceryl-phosphorylcholine (α-GPC) contribute to modulation physiology cells. A loss contributes development neurodegenerative diseases such as Alzheimer’s disease, multiple sclerosis, Parkinson’s disease. Our study has characterized cellular components reviewed effect lecithin, CDP-choline α-GPC documented in preclinical studies limited clinical trials these compounds. interesting results obtained with some CCPLs, particular α-GPC, probably would justify reconsideration most promising molecules larger attentively controlled studies. This can also better define role pathophysiology brain disorders by impairment.

Language: Английский

Citations

33
Role of pericytes in blood–brain barrier preservation during ischemia through tunneling nanotubes DOI Creative Commons
Francesco Pisani, Valentina Castagnola, Laura Simone

et al.

Cell Death and Disease, Journal Year: 2022, Volume and Issue: 13(7)

Published: July 5, 2022

Abstract Crosstalk mechanisms between pericytes, endothelial cells, and astrocytes preserve integrity function of the blood-brain-barrier (BBB) under physiological conditions. Long intercellular channels allowing transfer small molecules organelles distant cells called tunneling nanotubes (TNT) represent a potential substrate for energy matter exchanges tripartite cellular compartments BBB. However, role TNT across BBB conditions in course dysfunction is unknown. In this work, we analyzed TNT’s functional dialog human brain pericytes co-cultured with normal or after exposure to ischemia/reperfusion, condition which breakdown occurs, participate repair. Using live time-lapse fluorescence microscopy laser-scanning confocal microscopy, found that form long receive mitochondria from both cell types through mechanism. The mitochondrial also occurred multicellular assembloids reproduce three-dimensional architecture Under formation upregulated, exposed oxygen-glucose deprivation were rescued apoptosis by healthy TNT-mediated mitochondria, an effect was virtually abolished presence TNT-destroying drugs. results establish crosstalk demonstrate rescues ischemia/reperfusion-induced apoptosis. Our data confirm might play pivotal preserving structural repair diseases.

Language: Английский

Citations

31
Targetability of the neurovascular unit in inflammatory diseases of the central nervous system DOI

Brandon C. Smith,

Rachel A. Tinkey,

Benjamin C. Shaw

et al.

Immunological Reviews, Journal Year: 2022, Volume and Issue: 311(1), P. 39 - 49

Published: July 31, 2022

Summary The blood–brain barrier (BBB) is a selectively permeable separating the periphery from central nervous system (CNS). BBB restricts flow of most material into and out CNS, including many drugs that could be used as potent therapies. permeability modulated by several cells are collectively called neurovascular unit (NVU). NVU consists specialized CNS endothelial (ECs), pericytes, astrocytes, microglia, neurons. ECs maintain complex “seal” via tight junctions, forming BBB; breakdown these junctions leads to disruption. Pericytes control vascular within capillaries help basal lamina. Astrocytes much has moved beyond EC layer can form secondary under inflammatory conditions. Microglia survey border for noxious material. Neuronal activity also plays role in maintenance BBB. Since neurons all able modulate BBB, understating contributions each member will potentially uncover novel effective methods delivery neurotherapies CNS.

Language: Английский

Citations

31
Mural Cell SRF Controls Pericyte Migration, Vessel Patterning and Blood Flow DOI Creative Commons
Michael M. Orlich, Rodrigo Diéguez‐Hurtado, Regine Muehlfriedel

et al.

Circulation Research, Journal Year: 2022, Volume and Issue: 131(4), P. 308 - 327

Published: July 14, 2022

Pericytes and vascular smooth muscle cells, collectively known as mural are recruited through PDGFB (platelet-derived growth factor B)-PDGFRB receptor beta) signaling. MCs essential for integrity, their loss has been associated with numerous diseases. Most of this knowledge is based on studies in which insufficiently or fully absent upon inducible ablation. In contrast, little about the physiological consequences that result from impairment specific MC functions. Here, we characterize role transcription SRF (serum response factor) study its function developmental pathological contexts.We generated a mouse model MC-specific Srf gene deletion studied during retinal angiogenesis using RNA-sequencing, immunohistology, vivo live imaging, vitro techniques.By postnatal day 6, pericytes lacking were morphologically abnormal failed to properly comigrate angiogenic sprouts. As consequence, pericyte-deficient vessels at sprouting front became dilated leaky. By 12, also cells had lost SRF, coincided formation arteriovenous shunts. Mechanistically, show PDGFB-dependent activation mediated via MRTF (myocardin-related cofactors. We further MRTF-SRF signaling promotes pericyte ischemic retinopathy. experiments demonstrated regulates expression contractile SMC proteins maintain tone.SRF crucial distinct functions cells. directs migration downstream PDGFRB mediates machinery, triggers These roles contexts provide rationale novel therapeutic approaches targeting activity MCs.

Language: Английский

Citations

29