Cureus, Journal Year: 2025, Volume and Issue: unknown
Published: April 13, 2025
Language: Английский
Proceedings of the National Academy of Sciences, Journal Year: 2021, Volume and Issue: 118(51)
Published: Dec. 16, 2021
Significance Tumor heterogeneity is widely attributed to the imperfection of DNA replication. However, little known about mechanoregulation tumor heterogeneity. Here, we report that volumetric compression usually arises from progression increases overall gene-expression noise, leading differential cell-fate transitions along epithelial/mesenchymal transition regulatory network homogeneous non–small-cell lung carcinoma. The increased noise could be caused by a transit decrease in gene expression following decreasing cell volume under compression. Both experiments and numerical modeling confirmed hybrid state either epithelial or mesenchymal states stochastically. Thus, suggest cause its mechanical microenvironment as sensed cytoplasmic volume.
Language: Английский
Citations
33
Oncology Letters, Journal Year: 2022, Volume and Issue: 23(6)
Published: April 26, 2022
6-Gingerol is a bioactive compound isolated from Zingiber officinale. has been shown to have anticancer effects in numerous types of cancer cell. The mechanisms underlying the effect prostate requires investigation. In present study, on cell viability LNCaP, PC3 and DU145 cells were determined using MTT colony formation assays. significantly inhibited migration, adhesion invasion LPS-stimulated LPS-unstimulated cells. Furthermore, these changes accompanied by alterations protein expression levels epithelial-mesenchymal transition biomarkers, including E-cadherin, N-cadherin, Vimentin zonula occludens-1. also induced autophagy increasing LC3B-II Beclin-1 Combining with LY294002, an inhibitor, increased survival decreased glutathione (GSH) peroxidase 4 nuclear factor erythroid 2-related 2 Reactive oxygen species (ROS) but GSH following treatment Co-treatment ferroptosis ferrostatin-1, ROS 6-Gingerol-treated These results suggested that may via regulation ferroptosis. addition, EMT-related conclusion, induce protective autophagy, autophagic death ferroptosis-mediated findings provide strategy for prevention cancer.
Language: Английский
Citations
28
Cells, Journal Year: 2024, Volume and Issue: 13(3), P. 262 - 262
Published: Jan. 30, 2024
Noradrenaline and adrenaline, their cognate receptors, are currently accepted to participate in cancer progression. They may also initiation, although role this phase is much less explored. The aim of work was study the influence adrenergic stimulation several processes related breast carcinogenesis, using agonists MCF-10A non-tumorigenic cells. Activation β-adrenoceptors promoted an epithelial phenotype cells, revealed by increased expression marker E-cadherin a decrease mesenchymal markers, N-cadherin vimentin. cell motility migration were impaired after activation. Concomitant with effect, protrusions (lamellipodia filopodia) while increasing adhesion. decreases proliferation. When cells cultured under low attachment conditions, activation β- (likely β2) or α2-adrenoceptors had protective effects against death, suggesting pro-survival these adrenoceptors. Overall, our results showed that, adrenoceptor (mainly through β-adrenoceptors) be risk factor inducing some hallmarks, providing mechanistic explanation for increase incidences that associated conditions cause massive stimulation, such as stress.
Language: Английский
Citations
5
Biomaterials, Journal Year: 2023, Volume and Issue: 299, P. 122180 - 122180
Published: May 26, 2023
Language: Английский
Citations
11
Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)
Published: June 5, 2024
Abstract The extracellular matrix (ECM) is a dynamic and complex microenvironment that modulates cell behavior fate. Changes in ECM composition architecture have been correlated with development, differentiation, disease progression various pathologies, including breast cancer [1]. Studies shown aligned fibers drive pro-metastatic microenvironment, promoting the transformation of mammary epithelial cells into invasive ductal carcinoma via epithelial-to-mesenchymal transition (EMT) [2]. impact orientation on metabolism, however, largely unknown. Here, we employ two non-invasive imaging techniques, fluorescence-lifetime microscopy (FLIM) intensity-based multiphoton microscopy, to assess metabolic states cultured ECM-mimicking nanofibers random orientation. By tracking changes intrinsic fluorescence nicotinamide adenine dinucleotide flavin dinucleotide, as well expression levels metastatic markers, reveal how fiber alters metabolism EMT progression. Our study indicates cellular microenvironments play key role phenotypes evidenced by more glycolytic signature nanofiber scaffolds compared This finding particularly relevant for subsets marked high collagen remodeling (e.g. pregnancy associated cancer), may serve platform predicting clinical outcomes within these [3–6].
Language: Английский
Citations
4
Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 76, P. 101119 - 101119
Published: July 14, 2024
Language: Английский
Citations
4
Published: Feb. 6, 2025
Epithelial cells undergo epithelial–mesenchymal transition (EMT) during migration and regain their epithelial phenotype in the post-migration phase (mesenchymal– transition; MET). We established an experimental system that reproduced three-dimensional triphasic epithelia, i.e., original epithelium, its EMT, MET. Keratinocytes (KCs), skin cells, placed on a microporous membrane migrated through 3.0-µm or larger micropores. The 3.0-µm-pored induced structure with three states: stratified KCs above membrane, showing EMT within micropores, new epithelium under membrane. micropores failed to maintain epithelia. Live imaging revealed moved reciprocating manner, actin-rich filopodia-like KC structures extending into out of while unidirectionally Piezo1 keratin 6 were identified as negative modulators entry exit from These results demonstrate non-cancerous migrate confined spaces which might help form recapitulating wound healing processes.
Language: Английский
Citations
0
Published: Feb. 6, 2025
Epithelial cells undergo epithelial–mesenchymal transition (EMT) during migration and regain their epithelial phenotype in the post-migration phase (mesenchymal– transition; MET). We established an experimental system that reproduced three-dimensional triphasic epithelia, i.e., original epithelium, its EMT, MET. Keratinocytes (KCs), skin cells, placed on a microporous membrane migrated through 3.0-µm or larger micropores. The 3.0-µm-pored induced structure with three states: stratified KCs above membrane, showing EMT within micropores, new epithelium under membrane. micropores failed to maintain epithelia. Live imaging revealed moved reciprocating manner, actin-rich filopodia-like KC structures extending into out of while unidirectionally Piezo1 keratin 6 were identified as negative modulators entry exit from These results demonstrate non-cancerous migrate confined spaces which might help form recapitulating wound healing processes.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 11, 2025
Coordinated cell rotation along a curved matrix interface can sculpt epithelial tissues into spherical morphologies. Subsequently, radially-oriented invasion of multicellular strands or branches occur by local remodeling the confining matrix. These symmetry-breaking transitions emerge from dynamic reciprocity between cells and matrix, but remain poorly understood. Here, we show that spheroids collectively transition circumferential orbiting to radial via bi-directional interactions with surrounding curvature. Initially, exhibit an ellipsoidal shape become rounded as occurs. However, gradually reorient coordinated towards outward strand due accumulation contractile tractions at discrete sites. Remarkably, initial ellipsoid morphology predicts subsequent 2-4 roughly aligned major axis. We then perturb collective migration using osmotic pressure, showing be arrested reversed. also investigate in "mosaic" spheroids, small fraction "leader" weakened cell-cell adhesions impede still invade Finally, establish minimal self-propelled particle model elucidate how is mediated crosstalk cell-matrix adhesion boundary. Altogether, this work elucidates tissue morphogenesis governed interplay behaviors curvature cell-matrix, relevance for embryonic development tumor progression.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1799 - 1799
Published: Feb. 20, 2025
Primary or acquired resistance to therapeutic agents is a major obstacle in the treatment of cancer patients. Cervical fourth leading cause deaths among women worldwide and, despite advances screening and treatments, many patients with advanced stage cervical have high recurrence rate within two years standard treatment, drug being contributing factor. The development cell lines can facilitate comprehensive investigation mechanisms, which cannot be easily performed clinical trials. This study aimed create three novel robust (HeLa, CaSki, SiHa) fibroblast growth factor receptor (FGFR) tyrosine kinase inhibitor (PD173074). All drug-resistant (DR) overexpressed FGFR1, FGFR2, FGF2, FGF4, FGF7 proteins that were also localized nucleus. In addition, DR cells had significantly more aggressive phenotype (more migratory proliferative, less apoptotic) compared parental lines. These are critical tool for understanding molecular mechanisms underpinning identification potential biomarkers. Moreover, availability such may effective strategies using FGFR inhibitors combination other target pathways responsible inhibitors.
Language: Английский
Citations
0