Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 1 - 30

Published: April 23, 2024

Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.

Language: Английский

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Engineered exosomes and composite biomaterials for tissue regeneration

Theranostics, Journal Year: 2024, Volume and Issue: 14(5), P. 2099 - 2126

Published: Jan. 1, 2024

Exosomes, which are small vesicles enclosed by a lipid bilayer and released many cell types, widely dispersed have garnered increased attention in the field of regenerative medicine due to their ability serve as indicators diseases agents with therapeutic potential. Exosomes play crucial role mediating intercellular communication through transfer biomolecules, including proteins, lipids, RNA, other molecular constituents, between cells. The targeted transport proteins nucleic acids specific cells has potential enhance or impair biological functions. applications, they can be used alone combination approaches. examination unique attributes functions these factors emerged prominent study realm biomedical research. This manuscript summarizes origins properties exosomes, structural, biological, physical, chemical aspects. paper offers complete recent progress tissue repair medicine, emphasizing possible implications methods forthcoming regeneration attempts.

Language: Английский

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Hydrogel-based cardiac repair and regeneration function in the treatment of myocardial infarction

Materials Today Bio, Journal Year: 2024, Volume and Issue: 25, P. 100978 - 100978

Published: Feb. 13, 2024

A life-threatening illness that poses a serious threat to human health is myocardial infarction. It may result in significant number of cells dying, dilated left ventricles, dysfunctional heart function, and ultimately cardiac failure. Based on the development emerging biomaterials lack clinical treatment methods donors for infarction, hydrogels with good compatibility have been gradually applied Specifically, based three processes pathophysiology we summarized various types designed tissue engineering recent years, including natural hydrogels, intelligent growth factors, stem cells, microRNA-loaded hydrogels. In addition, also describe patch preparation techniques promote repair MI function. Although most these are still preclinical research stage trials, they great potential further application future. expected this review will improve our knowledge offer fresh approaches treating

Language: Английский

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Exosomal noncoding RNA: A potential therapy for retinal vascular diseases

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 35(1), P. 102128 - 102128

Published: Jan. 24, 2024

Exosomes are extracellular vesicles that can contain DNA, RNA, proteins, and metabolites. They secreted by cells play a regulatory role in various biological responses mediating cell-to-cell communication. Moreover, exosomes of interest developing therapies for retinal vascular disorders because they deliver substances to cellular targets. According recent research, be used as strategy managing diseases, being investigated therapeutic purposes eye conditions, including glaucoma, dry syndrome, ischemia, diabetic retinopathy, age-related macular degeneration. However, the exosomal noncoding RNA diseases is not fully understood. Here, we reviewed latest research on treating diseases. Research has shown RNAs, microRNAs, circular long RNAs significant regulation Furthermore, through exosome engineering, expression relevant controlled regulate Therefore, this review suggests could considered biomarker diagnosis target disease.

Language: Английский

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LncRNA HULC-miR-556-5p axis regulates cardiac microvascular endothelial cell function in chronic heart failure through the AMPK/FOXO3 pathway

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Purpose This study aimed to investigate the regulatory mechanism of miR-556-5p in endothelial cell injury associated with heart failure and its impact on function. Methods Human cardiac microvascular cells (HCMECs) were utilized as model. The expression levels related proteins assessed using techniques such real-time quantitative PCR Western blot. Cell apoptosis detection, analysis inflammatory cytokine release, viability assays, immunoblotting employed evaluate function physiological status. Results We observed a crucial role Ang II-induced HCMEC injury. Upregulation significantly suppressed release cytokines (such TNF-α, IL-1β, IL-6), while promoting survival. Further experimental results indicated that regulated by reducing level FOXO3 possibly modulating AMPK signaling pathway affect status cells. Additionally, markedly decreased cellular autophagy levels, further supporting failure. Conclusion elucidates important failure, providing new insights into pathophysiological mechanisms

Language: Английский

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Exosomes Derived from Apelin-Pretreated Mesenchymal Stem Cells Ameliorate Sepsis-Induced Myocardial Dysfunction by Alleviating Cardiomyocyte Pyroptosis via Delivery of miR-34a-5p

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 687 - 703

Published: Jan. 1, 2025

Background: Exosomes sourced from mesenchymal stem cells (MSC-EXOs) have become a promising therapeutic tool for sepsisinduced myocardial dysfunction (SMD).Our previous study demonstrated that Apelin pretreatment enhanced the benefit of MSCs in infarction by improving their paracrine effects.This aimed to determine whether EXOs Apelin-pretreated (Apelin-MSC-EXOs) would potent cardioprotective effects against SMD and elucidate underlying mechanisms.Methods: MSC-EXOs Apelin-MSC-EXOs were isolated identified.Mice neonatal cardiomyocytes (NCMs) treated with or under lipopolysaccharide (LPS) condition vitro.Cardiomyocyte pyroptosis was determined TUNEL staining.RNA sequencing used identify differentially expressed functional miRNAs between Apelin-MSC-EXOs.MSC-EXOs transplanted into mouse model induced cecal ligation puncture (CLP) via tail vein.Heart function evaluated echocardiography.Results: Compared MSC-EXOs, Apelin-MSC-EXO transplantation greatly cardiac mice.Both suppressed cardiomyocyte vivo vitro, latter exhibiting superior protective effects.miR-34a-5p effectively mediated exert high mobility group box-1 (HMGB1) as potential target.Mechanistically, delivered miR-34a-5p injured cardiomyocytes, thereby ameliorating regulation HMGB1/AMPK axis.These partially abrogated downregulation Apelin-MSC-EXOs.Conclusion: Our revealed key component protected mediation signaling pathway.

Language: Английский

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Programmed cardiomyocyte death in myocardial infarction

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Language: Английский

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Therapeutic Potential of Gasdermin D‐Mediated Myocardial Pyroptosis in Ischaemic Heart Disease: Expanding the Paradigm From Bench to Clinical Insights

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(3)

Published: Feb. 1, 2025

ABSTRACT Ischaemic heart disease (IHD) remains a leading cause of global morbidity and mortality. One significant contributor to the pathology IHD is excessive release inflammatory mediators during progression. Pyroptosis form programmed cell death (PCD) triggered by activation inflammasomes caspase 1. The 1 proteolytically cleaves gasdermin D (GSDMD) activated amino acid terminus (GSDMD‐NT), disruption plasma membrane. This cascade events considered canonical pathway pyroptosis. also caused oxidative stress, thereby triggering noncanonical pyroptosis via caspases 4/5/11. Previous studies have provided compelling evidence close relationship between aetiology (e.g., acute myocardial infarction, ischaemia reperfusion injury chronic infarction), as well association with unfavourable clinical outcomes. Several interventions aimed at targeting demonstrated promising therapeutic benefits against IHD‐related pathologies. review provides mechanistic insights into roles in from vitro, vivo perspectives. In‐depth understanding this area could pave way for future development novel strategies IHD.

Language: Английский

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Two promising approaches in the treatment of myocardial infarction: stem cells and gene therapy

Frontiers in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 19, 2025

Cardiovascular diseases (CVDs), characterized by a high incidence rate and mortality, have become the leading cause of death globally. CVDs include coronary heart disease, stroke, hypertension, peripheral vascular diseases. In China, ranks first in all major At present, main methods to treat ischemic disease are drug therapy, intervention operation. These only alleviate symptoms failure myocardial ischemia improve patients' quality life partially restoring reperfusion. Due extensive irreversible necrosis cells caused hypoxia, these cannot reverse damage, resulting suboptimal long-term outcomes. Although mature cardiomyocytes been proved not be terminally differentiated cells, they very limited ability regeneration proliferation, so can completely replace damaged myocardium restore contractile function. transplantation heart, its clinical application promotion source donor, expensive cost, immune rejection, ethical problems. It has an urgent task for medicine seek new better treatment. The content this paper is explore stem gene technology treatment infarction (MI).

Language: Английский

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Exosome-Based Therapy in Cardiovascular Diseases: A New Frontier in Cardiovascular Disease Treatment

Korean Circulation Journal, Journal Year: 2025, Volume and Issue: 55

Published: Jan. 1, 2025

Exosomes, small extracellular vesicles ranging from 30 to 150 nanometers in diameter, have emerged as pivotal mediators of intercellular communication. These vesicles, originally perceived cellular debris, are now recognized for their intricate roles transporting bioactive molecules, including proteins, lipids, and nucleic acids, between cells. Exosomes received considerable attention due diverse physiological pathological processes, especially relation cardiovascular diseases (CVDs). CVDs intricately linked, sharing common risk factors mechanisms, such inflammation, oxidative stress, endothelial dysfunction. been implicated either directly or indirectly influencing these phenomena. They secreted by virtually all cell types, cells, cardiomyocytes, stem play critical maintaining vascular homeostasis responding stimuli. Their capacity traverse biological barriers, maintain stability circulation, effectively encapsulate deliver a variety molecular cargos makes them promising candidates both biomarkers therapeutic agents. This review aims explore the multifaceted exosomes CVDs. And we will discuss mechanisms exosome biogenesis release, composition, ways which they contribute disease pathophysiology. Additionally, emphasize potential diagnostic uses, highlighting significance advancement innovative treatment strategies. explores recent findings advancements research, emphasizing CVD paving way future studies clinical applications.

Language: Английский

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