Investigating the role of IDO1 in tumors: correlating IDO1 expression with clinical pathological features and prognosis in lung adenocarcinoma patients

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18776 - e18776

Published: Feb. 19, 2025

This study aimed to investigate the role and expression patterns of IDO1 in various tumors, focusing on its correlation with clinical pathological characteristics prognosis patients specifically diagnosed lung adenocarcinoma. Pan-cancer analysis assessed function across different tumor types. Bioinformatics tools, immunohistochemistry techniques, statistical analyses were employed evaluate levels their association features was found be significantly overexpressed types higher correlating poorer progression-free survival (PFS) overall (OS). In adenocarcinoma patients, protein predominantly localized cytoplasm cell membrane cells, observed cells closer normal tissue. Statistical revealed no significant differences based patient's data, including gender, age, location, allergy history, hypertension cardiovascular disease diabetes (both type 1 2), body mass index, smoking family alcohol maximum diameter (P > 0.05). However, positively correlated lymph node metastasis, pleural invasion, recurrence, lower differentiation, solid components, preoperative chemotherapy, tumor, node, metastasis (TNM) staging (*P < 0.05), while negatively prior surgical history Patients exhibiting high demonstrated worse PFS OS (***P 0.001 **P = 0.003, respectively). High correlates increased invasiveness, metastatic potential, advanced stage, prognosis.

Language: Английский

Hypoxia-Induced VGF Promotes Cell Migration and Invasion in Prostate Cancer via the PI3K/Akt Axis

Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(2)

Published: Feb. 20, 2025

Metastasis is a major cause of prostate cancer (PCa)-related deaths in men. Recent studies have indicated that VGF nerve growth factor inducible (VGF) affects tumor invasion and metastasis. The present study investigated whether abnormally expressed PCa progression the specific regulatory mechanisms by which sh- hypoxia-inducible factor1 alpha (HIF-1α) plasmid was transfected into human cell lines 22Rv1 C4-2 to create with stable low expression overexpression VGF. Quantitative PCR (qPCR) performed detect mRNA. Western blot invasive migration-related proteins. Akt activator SC79 (4 μg/mL) added. After adding docetaxel nM) cells sh-NC sh-VGF, capacity migrate invasively assessed using Transwell scratch assays. Nude mice were injected stably or sh-VGF metastasis detected live imaging HE staining after injection (10 mg/kg). Abnormal levels tissue plasma samples detected, knockdown suppressed also shown affect via PI3K/Akt signaling. limited inhibitory impact higher when paired (p < 0.001). hypoxia induction, both mRNA protein HIF-1α increased, associated poor prognosis for PCa. By stimulating pathway, encourages As result, targeting may be potential treatment approach metastatic therapy.

Language: Английский

PTK2B inhibitor PF-431396 inhibits inflammatory response and apoptosis of ovarian granulosa cells by targeting AKT1 phosphorylation in premature ovarian insufficiency

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 155, P. 114651 - 114651

Published: April 14, 2025

Language: Английский