Current Research in Drug-Free Cancer Therapies

Bioengineering, Journal Year: 2025, Volume and Issue: 12(4), P. 341 - 341

Published: March 26, 2025

Cancer treatment has historically depended on conventional methods like chemotherapy, radiation, and surgery; however, these strategies frequently present considerable limitations, including toxicity, resistance, negative impacts healthy tissues. In addressing challenges, drug-free cancer therapies have developed as viable alternatives, utilizing advanced physical biological to specifically target tumor cells while reducing damage normal This review examines several strategies, such high-intensity focused energy beams, nanosecond pulsed electric fields, photothermal therapy well the use of inorganic nanoparticles promote selective apoptosis. We also investigate significance targeting microenvironment, precision medicine, immunotherapy in progression personalized therapies. Although approaches demonstrate significant promise, challenges scalability, safety, regulatory obstacles must be resolved for clinical application. paper presents an overview current research therapies, emphasizing recent advancements, underlying scientific principles, steps required implementation.

Language: Английский

Plasma-Activated Medium Inhibited the Proliferation and Migration of Non-Small Cell Lung Cancer A549 Cells in 3D Culture

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13262 - 13262

Published: Dec. 10, 2024

Lung cancer is the most common type of malignant tumor worldwide. Plasma-activated medium (PAM) an innovative treatment method that has received considerable scientific attention. The objective this study to evaluate effects PAM on anti-tumor characteristics non-small cell lung (NSCLC) cells in two-dimensional (2D) and three-dimensional (3D) cultures. proliferative migratory capabilities A549 2D 3D cultures were assessed using MTT, migration, invasion assays, cycle, respectively. also investigated impact changes content intracellular extracellular reactive species analyzed protein expression Western Blot method. inhibited viability, abilities both cultures, suppressed epithelial–mesenchymal transition (EMT) process, downregulated RAS/ERK signaling pathway, which effectively spheroid formation. Additionally, effect was mediated through ROS-induced oxidative reactions, exhibited greater cytotoxicity culture compared culture. As 2D, model provides a viable vitro for studying mechanisms cancer. represents effective new NSCLC.

Language: Английский