Published: Jan. 1, 2024
Language: Английский
Theriogenology, Journal Year: 2025, Volume and Issue: 239, P. 117384 - 117384
Published: March 10, 2025
Language: Английский
Citations
0
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2025, Volume and Issue: 16(1)
Published: April 15, 2025
Abstract Polyamines (putrescine, spermidine, and spermine) are aliphatic compounds ubiquitous in prokaryotes eukaryotes. Positively charged polyamines bind to negatively macromolecules, such as nucleic acids acidic phospholipids, involved physiological activities including cell proliferation, differentiation, apoptosis gene regulation. Intracellular polyamine levels regulated by biosynthesis, catabolism transport. the body originate from two primary sources: dietary intake intestinal microbial metabolism. These then transported into bloodstream, through which they distributed various tissues organs exert their biological functions. synthesized microorganisms serve dual critical roles. First, essential for maintaining concentrations within digestive tract. Second, transcriptional post-transcriptional mechanisms, these microbial-derived modulate expression of genes governing key processes epithelial cells—including migration, apoptosis, cell–cell interactions. Collectively, regulatory effects help maintain homeostasis ensure integrity gut barrier. In addition, interact with microbiota promoting growth, biofilm formation, swarming, endocytosis vesicle production, etc. Supplementation has been demonstrated be important regulating host composition, enhancing nutrient absorption, improving metabolism immunity. this review, we will focus on recent advances study transport microbes cells. We summarize scientific understanding roles homeostasis, exploring cellular molecular mechanisms potential clinical applications, providing a rationale an target treatment intestinal-based diseases. Graphical
Language: Английский
Citations
0
Journal of Ovarian Research, Journal Year: 2025, Volume and Issue: 18(1)
Published: March 12, 2025
With the development of modern society and prolonged education, more women choose to delay their childbearing age, which greatly increases number aged older than 35 years with needs. However, increasing quantity quality oocytes continue fall, especially aneuploidy, leads a low in vitro fertilization (IVF) success rate, high abortion rate teratogenesis assisted reproduction advanced maternal age. In addition genetics epigenetics, follicular metabolism homeostasis is closely related ovarian aging oocyte aneuploidy. Glucose, lipid, amino acid not only provide energy for follicle genesis but also regulate maturation. This review focuses on relationships among metabolism, discusses potential therapeutic metabolites aging.
Language: Английский
Citations
0
Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)
Published: April 17, 2025
Post-ovulatory aging (POA) is associated with reduced fertilization rates and poor embryo quality both in vivo vitro. However, the relationship between nicotinamide adenine dinucleotide (NAD+) filamentous actin (F-actin) cytoskeleton POA-induced oocytes remains unknown. Here, we investigated mechanisms by which NAD+ salvage pathways function oocyte maturation upon POA through F-actin cytoskeleton. Porcine were aged extending vitro (IVM) for an additional 24 h to create a model. adducin 1 (ADD1)-related spindle assembly analyzed using immunofluorescence, western blotting, RNA sequencing identify key gene categories IVM groups. To assess restoring maturation, mononucleotide (NMN) was added efficiency evaluated. Expression of factors, markers, NAD+-related genes via quantitative polymerase chain reaction, blotting. We revealed unique interactions F-actin/ADD1-related factors (clusterin (CLU) FAM111 trypsin-like peptidase A (FAM111A)) poor-quality oocytes. established extension based on 44 IVM. They exhibited collapses abnormal cortical F-actin, ADD1, acetyl(Ac)-α-tubulin protein levels, resulted defective assembly. analysis performed differentially expressed involved viability response aging, cytoskeleton, NAD metabolic processes and/or This showed that NAD-binding after induction, eight downregulated compared Importantly, activation addition NMN medium at rescued capability perturbations F-actin. polymerization generated from essential factor determining quality. effect mediated microtubules related
Language: Английский
Citations
0
Biology of Reproduction, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 23, 2024
Abstract Natural ovarian aging is one of the major causes for declining fertility in female animals, which has become an insurmountable issue human reproduction clinics and assisted reproductive technology procedures. Nevertheless, molecular basis oocyte remains poorly understood, feasible improvement strategies are unavailable. In present study, vivo supplementation pyrroloquinoline-quinone effectively elevated fecundity reproductively aged mice by balancing hormonal secretion, harmonizing estrus cycle, eliminating fibrosis. Moreover, quality also increased after administration from various aspects, including nuclear cytoplasmic maturation competency, fertilization capacity, pre-implantation embryonic development potential. Transcriptomic analysis identified target pathways that might mediate pyrroloquinoline-quinone’s effects oocytes. Specifically, it was demonstrated restored mitochondrial dynamics lysosomal function to remove excessive reactive oxygen species suppress apoptosis Jointly, these findings demonstrate efficacious method restore compromised ovary damaged mice, be a potential clinical therapy women advanced maternal age with infertility.
Language: Английский
Citations
1
Published: Jan. 1, 2024
Language: Английский
Citations
0