Identification and validation of CDC20 and ITCH as ubiquitination related biomarker in idiopathic pulmonary fibrosis DOI Creative Commons

Shulei Sun,

Yubao Wang,

Jing Feng

et al.

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: April 1, 2025

Abstract Purpose Ubiquitination plays a crucial role in various diseases. This study aims to explore the potential ubiquitination related genes IPF. Methods The gene microarray dataset GSE24206 was obtained from GEO database. Subsequently, through differential expression analysis and molecular signatures database, we 1734 differentially expressed 742 genes. Through venn diagram analysis, 53 Then, gene-ontology (GO) enrichment Kyoto Encyclopedia of Genes Genomes (KEGG) pathway protein-protein interactions (PPI) set (GSEA) were applied for Finally, CDC20 ITCH IPF patients cells validated by qPCR western blot assay. Results A total (36 up-regulated 17 down-regulated genes) identified between 6 healthy controls. GO KEGG mainly involved regulation protein ubiquitination, post-translational modification ubiquitin mediated proteolysis. PPI results demonstrated that these interacted with each other. GSEA some hub epithelial mesenchymal transition, inflammatory response, hypoxia, apoptosis. experiment level consistent bioinformatics results. Conclusion We analysis. other may influence development transition response. Our research findings provide insights into mechanisms fibrosis evidence therapeutic targets fibrosis.

Language: Английский

Post‐COVID‐19 pulmonary fibrosis: Mechanisms, biomarkers, and therapeutic perspectives DOI Creative Commons

Urvinder Kaur Sardarni,

Siddappa N. Byrareddy

Clinical and Translational Discovery, Journal Year: 2025, Volume and Issue: 5(1)

Published: Feb. 1, 2025

Abstract Post‐COVID‐19 pulmonary fibrosis (post‐CPF) has emerged as a serious complication with profound implications for long‐term respiratory health. This short review explores the multifactorial mechanisms underlying post‐CPF, emphasising role of oxidative stress, epithelial‐to‐mesenchymal transition (EMT), and dysregulated immune responses. Key signalling pathways, such TGF‐β, WNT, Cadherin, are pivotal in progression, offering potential therapeutic targets. Biomarkers, MUC4, KRT5, ATP12A show promise early detection targeting, they share molecular features idiopathic (IPF) fibrotic interstitial lung diseases (f‐ILDs), suggesting opportunities to repurpose antifibrotic therapies. Despite these advancements, significant gaps remain understanding cellular hindering effective management post‐CPF. Addressing challenges through targeted approach is critical improving outcomes survivors severe COVID‐19.

Language: Английский

Citations

0
Identification and validation of CDC20 and ITCH as ubiquitination related biomarker in idiopathic pulmonary fibrosis DOI Creative Commons

Shulei Sun,

Yubao Wang,

Jing Feng

et al.

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: April 1, 2025

Abstract Purpose Ubiquitination plays a crucial role in various diseases. This study aims to explore the potential ubiquitination related genes IPF. Methods The gene microarray dataset GSE24206 was obtained from GEO database. Subsequently, through differential expression analysis and molecular signatures database, we 1734 differentially expressed 742 genes. Through venn diagram analysis, 53 Then, gene-ontology (GO) enrichment Kyoto Encyclopedia of Genes Genomes (KEGG) pathway protein-protein interactions (PPI) set (GSEA) were applied for Finally, CDC20 ITCH IPF patients cells validated by qPCR western blot assay. Results A total (36 up-regulated 17 down-regulated genes) identified between 6 healthy controls. GO KEGG mainly involved regulation protein ubiquitination, post-translational modification ubiquitin mediated proteolysis. PPI results demonstrated that these interacted with each other. GSEA some hub epithelial mesenchymal transition, inflammatory response, hypoxia, apoptosis. experiment level consistent bioinformatics results. Conclusion We analysis. other may influence development transition response. Our research findings provide insights into mechanisms fibrosis evidence therapeutic targets fibrosis.

Language: Английский

Citations

0