Decoding dengue’s neurological assault: insights from single-cell CNS analysis in an immunocompromised mouse model

Journal of Neuroinflammation, Journal Year: 2025, Volume and Issue: 22(1)

Published: March 4, 2025

Dengue encephalitis, a severe neurological complication of dengue virus infection, is increasingly recognized for its rising incidence and significant public health burden. Despite growing prevalence, the underlying mechanisms effective therapeutic strategies remain poorly understood. Cellular atlas encephalitis was determined by single-nucleus RNA sequencing. Viral load level cytokines expression detected RT-qPCR. The target cells were verified immunofluorescence. cytotoxic effect CD8+ T cell flow cytometry, immunofluorescence, in vivo depletion, adoptive transfer CCK-8-based viability assay. Axonal synaptic reduction induced infection demonstrated RT-qPCR, Western blot, transmission electron microscope Finally, motor sensory functions mice open field test hot plate test, respectively. In this study, we utilized sequencing on brain tissues from dengue-infected murine model to construct comprehensive cellular encephalitis. Our findings identify neurons, particularly inhibitory GABAergic subtypes, as primary targets virus. Additionally, immune infiltration observed, contributing damage. Comprehensive analyses cell-cell communication, combined with depletion restoration experiments, have elucidated critical role triggering through their interaction neurons. These infiltrate peripheral circulation, interact induce damage synapse axon, accompanied dysfunction. We defined mouse identified neuron addition, cell, which leads apoptosis dysfunction mice. study provides molecular framework understanding advanced technologies. insights gained serve foundation future investigations into pathogenesis development targeted approaches.

Language: Английский

Baicalin Relieves Glaesserella parasuis-Triggered Immunosuppression Through Polarization via MIF/CD74 Signaling Pathway in Piglets

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 640 - 640

Published: April 29, 2025

Glaesserella parasuis (G. parasuis) infection is responsible for Glässer’s disease in pigs. G. could trigger piglet immunosuppression, but the mechanism of inducing immunosuppression by remains unknown. Macrophage migration inhibitory factor (MIF)/CD74 axis has been shown to participate inflammation response and function MIF/CD74 during elicited not fully explored. This experiment explored efficacy baicalin on alleviation through regulating polarization via signaling pathway. Our data indicated that reduced IL-1β, IL-6, IL-8, IL-18, TNF-α, COX-2 expression, regulated expression spleen. Immunohistochemistry analysis showed enhanced CD74 protein levels spleen piglets induced parasuis. Baicalin PI3K/Akt/mTOR pathway RAF/MEK/ERK activation, modified autophagy-related proteins Beclin-1, P62, LC3B, promoted M2 M1 polarization, CD3, CD4, CD8, TIM3 study reveals important functions parasuis-induced may offer a new therapeutic method control infection.

Language: Английский

Research progress on tumor-infiltrating lymphocyte therapy for cervical cancer

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 19, 2025

Cervical cancer is a common malignant tumor in women, and human papillomavirus (HPV) infection major cause of cervical cancer. Tumor-infiltrating lymphocytes (TILs) are heterogeneous group primarily composed T found within the parenchyma stroma. These cells can be isolated from tissue, activated, expanded vitro , reinfused into patient to exert an anti-tumor immune effect. As form personalized immunotherapy, TILs therapy has shown satisfactory efficacy safety advanced recurrent metastatic cancer, offering new hope patients with However, for still faces several limitations challenges. This article reviews process latest developments discusses challenges usage prospects this treatment.

Language: Английский