Figshare, Journal Year: 2023, Volume and Issue: unknown
Published: Jan. 1, 2023
Additional file 1. Correlation of chronological age with DNA methylation using Horvath, Hannum, SkinandBlood, PhenoAge, and GrimAge clocks the methylation-based telomere length (TL) estimator.
Language: Английский
npj Parkinson s Disease, Journal Year: 2023, Volume and Issue: 9(1)
Published: Oct. 31, 2023
Abstract Understanding the contribution of immune mechanisms to Parkinson’s disease pathogenesis is an important challenge, potentially major therapeutic implications. To further elucidate involvement peripheral cells, we studied epigenome-wide DNA methylation in isolated populations CD14 + monocytes, CD19 B CD4 T and CD8 cells from patients healthy control participants. We included 25 with a maximum five years duration controls, four cell each fresh blood sample. Epigenome-wide profiles were generated 186 samples using Illumina MethylationEpic array association status was tested linear regression models. identified six differentially methylated CpGs monocytes one cells. Four regions including region upstream RAB32 , gene that has been linked LRRK2 . Methylation correlated negatively mRNA expression, expression upregulated both our summary statistics previous study. Our study early provides evidence for changes across different types, highlighting locus. The findings predominantly cell-type-specific, demonstrating value isolating purified genomic studies.
Language: Английский
Citations
10
Clinical Epigenetics, Journal Year: 2023, Volume and Issue: 15(1)
Published: Nov. 28, 2023
Aging has been reported as a major risk factor for severe symptoms and higher mortality rates in COVID-19 patients. Molecular hallmarks such epigenetic alterations telomere attenuation reflect the biological process of aging. Epigenetic clocks have shown to be valuable tools measuring age various tissues samples. As such, these can determine accelerated aging time-to-mortality across tissues. Previous reports attrition acceleration following SARS-CoV-2 infection. However, effect on outcome (death/recovery) patients with acute respiratory distress syndrome (ARDS) not well investigated.
Language: Английский
Citations
10
BMC Pulmonary Medicine, Journal Year: 2024, Volume and Issue: 24(1)
Published: Jan. 2, 2024
Abstract Background Chronic respiratory diseases, such as chronic obstructive pulmonary disease (COPD) and bronchiectasis, present significant threats to global health. Recent studies have revealed the crucial role of lung microbiome in development these diseases. Pathogens evolved complex strategies evade immune response, with manipulation host cellular epigenetic mechanisms playing a pivotal role. There is existing evidence regarding effects Pseudomonas on modifications their association Therefore, this study aims directly assess connection between abundance We hope that our findings will shed light molecular behind pathogen infections. Methods analyzed data from 366 participants, including individuals COPD, acute exacerbations COPD (AECOPD), healthy individuals. Previous given limited attention impact groups comparison Two independent datasets different ethnic backgrounds were used for external validation. Each dataset separately bacteria at genus level. Results The reveals , bacterium, was consistently found high concentrations all but it very low datasets. This suggests may influence through epigenetics, contributing progression Conclusions emphasizes importance understanding relationship microbiome, onset disease. Enhanced recognition functions, along better concepts, can lead improved diagnosis treatment.
Language: Английский
Citations
3
Biology, Journal Year: 2024, Volume and Issue: 13(4), P. 245 - 245
Published: April 7, 2024
Earlier research has established the existence of reliable interactive genomic biomarkers. However, DNA methylation biomarkers, not to mention interactivity, have yet be identified at epigenetic level. This study, drawing from 865,859 sites, discovered two miniature sets Infinium MethylationEPIC each having eight CpG sites (genes) interact with other and disease subtypes. They led nearly perfect (96.87–100% accuracy) prediction COVID-19 patients diseases or healthy controls. These can jointly explain some post-COVID-19-related conditions. optimally performing biomarkers reported in literature become potential druggable targets. Among these cg16785077 (gene MX1), cg25932713 PARP9), cg22930808 PARP9) levels indicate that initial SARS-CoV-2 virus may better treated as a transcribed viral into RNA virus, i.e., an concerned scientists field. Such discovery significantly change scientific thinking knowledge viruses.
Language: Английский
Citations
3
Clinical Epigenetics, Journal Year: 2024, Volume and Issue: 16(1)
Published: Aug. 20, 2024
The epigenetic status of patients 6-month post-COVID-19 infection remains largely unexplored. existence long-COVID, or post-acute sequelae SARS-CoV-2 (PASC), suggests potential long-term changes. Long-COVID includes symptoms like fatigue, neurological issues, and organ-related problems, regardless initial severity. mechanisms behind long-COVID are unclear, but virus-induced changes could play a role. Our study explores the lasting impacts infection. We analyzed genome-wide DNA methylation patterns in an Italian cohort 96 6 months after COVID-19 exposure, comparing them to 191 healthy controls. identified 42 CpG sites with significant differences (FDR < 0.05), primarily within islands gene promoters. Dysregulated genes highlighted links glutamate/glutamine metabolism, which may be relevant PASC symptoms. Key significance effects include GLUD1, ATP1A3, ARRB2. Furthermore, Horvath's clock showed slight age acceleration patients. also observed substantial increase stochastic mutations (SEMs) group, implying drift. SEM analysis 790 affected genes, indicating dysregulation pathways related insulin resistance, VEGF signaling, apoptosis, hypoxia response, T-cell activation, endothelin signaling. provides valuable insights into consequences COVID-19. Results suggest possible associations accelerated aging, drift, disruption critical biological linked immune vascular health. Understanding these crucial for elucidating complex developing targeted therapeutic interventions.
Language: Английский
Citations
3
International Journal of Molecular Medicine, Journal Year: 2024, Volume and Issue: 55(3)
Published: Dec. 27, 2024
Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is an inflammatory response arising from and systemic with diverse causes associated high rates of morbidity mortality. To date, no fully effective pharmacological therapies have been established the relevant underlying mechanisms warrant elucidation, which may be facilitated by multi‑omics technology. The present review summarizes application technology in identifying novel diagnostic markers therapeutic strategies ALI/ARDS as well its pathogenesis.
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: July 19, 2023
Abstract Aging has been reported as a major risk factor for severe symptoms and higher mortality rates in COVID-19 patients. Molecular hallmarks such epigenetic alterations telomere attenuation reflect the biological process of aging. Epigenetic clocks have shown to be valuable tools measuring age variety tissues samples. As such, these can determine accelerated aging time-to-mortality across various tissues. Previous reports attrition acceleration following SARS-CoV-2 infection. However, effect on outcome (death/recovery) patients with Acute Respiratory Distress Syndrome (ARDS) not well investigated. In this study, we measured DNA methylation 87 cases ARDS under mechanical ventilation. Furthermore, compared dynamic changes multiples time-points until recovery or death. was using Horvath, Hannum, DNAm skin blood, GrimAge, PhenoAge clocks, whereas length calculated surrogate marker DNAmTL. Our analysis revealed significant but no cases. addition, observed deceleration at inclusion vs end follow-up recovered deceased certain clocks. When comparing (EAA), detected EAA both Horvath The DNAmTL measurements COVID19 between change when who those died. conclusion, associated treatment hospitalized Patients ARDS. A better understanding long-term effects how they might contribute Long COVID individuals is urgently needed.
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: April 30, 2024
Abstract The emergence of SARS-CoV-2 has had a profound adverse impact on global health and continues to remain threat worldwide. disease spectrum COVID-19 ranges from asymptomatic fatal clinical outcomes especially in the elderly population individuals with underlying medical conditions. host immune responses cells at protein DNA levels remains largely ambiguous. In case-control study, here we explored methylation patterns upper respiratory airway determine how infection altered status requiring hospitalization for COVID-19. We performed arrays nasopharyngeal swabs inclusion/hospitalization as well 6 weeks post-inclusion. Our study reveals distinct pattern patients compared healthy controls, characterized by 317 779 differentially methylated CpGs. Notably, within transcription start sites gene body, exhibited higher number genes/CpGs elevated levels. Enrichment analysis genes highlighted associated inflammatory functions. Some -induced CpG methylations were transient, returning normal Enriched interest included IL-17A, pivotal cytokine implicated inflammation healing, NUP93, antiviral innate immunity. Further, six our data set, OAS1, CXCR5, APP, CCL20, CNR2, C3AR1, found enrichment previous studies. Additionally, RNAse1 RNAse2 emerged key regulators, while IL-18 played role various biological processes patients. Overall, results demonstrates that major modifying many this could have implications conditioning airways individual response future infections.
Language: Английский
Citations
0
Published: Sept. 23, 2024
Language: Английский
Citations
0
GeroScience, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 5, 2024
Abstract The COVID-19 pandemic has left a lasting legacy on human health, extending beyond the acute phase of infection. This article explores evidence suggesting that SARS-CoV-2 infection can induce persistent epigenetic modifications, particularly in DNA methylation patterns, with potential long-term consequences for individuals’ health and aging trajectories. review discusses methylation-based biomarkers, such as clocks, to identify individuals at risk accelerated tailor personalized interventions. Integrating clock analysis into clinical management could mark new era treatment COVID-19, possibly helping clinicians understand patient susceptibility severe outcomes establish preventive strategies. Several valuable reviews address role epigenetics infectious diseases, including Sars-CoV-2 However, this provides an original overview current understanding dimensions offering insights implications pandemic. While acknowledging limitations data, we emphasize need future research unravel precise mechanisms underlying COVID-19-induced changes explore approaches target these modifications. Graphical Abstract: Impact landscape individual response Following infection, may develop either normal immune or aberrant one, cytokine storm. Both scenarios result long-lasting consequences, known “long COVID.” condition reshape by altering contributing “epigenetic drift.” drift, further influenced various factors, lead gene expression, functionality, disease susceptibility. One significant consequence drift is acceleration biological aging, which profoundly impact medical Created BioRender.com.
Language: Английский
Citations
0