International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(5), P. 2107 - 2107
Published: Feb. 27, 2025
Macrophages
are
highly
adaptable
immune
cells
capable
of
responding
dynamically
to
diverse
environmental
cues.
They
pivotal
in
maintaining
homeostasis,
orchestrating
responses,
facilitating
tissue
repair,
and,
under
certain
conditions,
contributing
disease
pathogenesis.
This
review
delves
into
the
complex
biology
macrophages,
highlighting
their
polarization
states,
roles
autoimmune
and
inflammatory
diseases,
involvement
cancer
progression,
potential
as
therapeutic
targets.
By
understanding
context-dependent
functional
plasticity
we
can
better
appreciate
contributions
health
disease,
paving
way
for
innovative
strategies.
Genes,
Journal Year:
2023,
Volume and Issue:
14(10), P. 1856 - 1856
Published: Sept. 24, 2023
The
development
of
cancer
begins
with
cells
transitioning
from
their
multicellular
nature
to
a
state
akin
unicellular
organisms.
This
shift
leads
breakdown
in
the
crucial
regulators
inherent
multicellularity,
resulting
emergence
diverse
cell
subpopulations
that
have
enhanced
adaptability.
presence
different
within
tumour,
known
as
intratumoural
heterogeneity
(ITH),
poses
challenges
for
treatment.
In
this
review,
we
delve
into
dynamics
multicellularity
unicellularity
during
onset
and
progression.
We
highlight
role
genetic
non-genetic
factors,
well
tumour
microenvironment,
promoting
ITH
evolution.
Additionally,
shed
light
on
latest
advancements
omics
technologies
allow
in-depth
analysis
tumours
at
single-cell
level
spatial
organization
tissue.
Obtaining
such
detailed
information
is
deepening
our
understanding
evolutionary
paths
cancer,
allowing
effective
therapies
targeting
key
drivers
BMC Urology,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: July 16, 2024
Abstract
Background
Insulin-like
growth
factor
2
mRNA-binding
protein
(IGF2BP2)
has
been
confirmed
to
play
oncogenic
role
in
many
cancers.
However,
the
and
mechanism
of
IGF2BP2
bladder
cancer
(BCa)
still
deserves
be
further
revealed.
Methods
The
mRNA
levels
neuronilin-1
(NRP1)
were
detected
by
real-time
quantitative
PCR
(RT-qPCR)
western
blot.
Cell
proliferation,
apoptosis,
migration
invasion
determined
using
colony
formation
assay,
EdU
CCK8
flow
cytometry
transwell
assay.
Xenograft
tumor
model
was
conducted
evaluate
vivo.
THP-1-M0
macrophages
co-cultured
with
condition
medium
(CM)
BCa
cells
induce
polarization.
M2
macrophage
polarization
assessed
detecting
markers
RT-qPCR
measuring
proportion
cytometry.
Moreover,
MeRIP
RIP
assay
performed
assess
m6A
level
interaction
between
NRP1.
Results
NRP1
upregulated
tissues
cells.
knockdown
suppressed
cell
metastasis,
as
well
inhibited
growth.
After
CM
cells,
markedly
enhanced,
while
this
effect
abolished
knockdown.
positively
correlated
level,
it
could
increase
stability.
overexpression
reversed
suppressive
on
progression.
Conclusion
m6A-reader
enhanced
progression
promoting
