Frontiers in Bioscience-Landmark,
Journal Year:
2024,
Volume and Issue:
29(10)
Published: Oct. 12, 2024
Background:
Gastric
cancer
(GC)
is
a
significant
global
health
burden
with
limited
treatment
options.
The
purpose
of
this
study
was
to
investigate
the
role
SLC30A2,
zinc
transporter,
in
GC
development
and
its
capacity
as
target
for
therapy.
Methods:
A
comprehensive
analysis
datasets
(GSE54129
stomach
adenocarcinoma
(STAD)
from
Cancer
Genome
Atlas
(TCGA))
conducted
using
bioinformatics
tools
examine
differential
gene
expression,
focusing
on
SLC30A2.
Functional
assays,
including
Cell
counting
kit-8
(CCK-8)
transwell
were
carried
out
cell
lines
determine
impact
SLC30A2
knockdown
behavior.
Flow
cytometry
utilized
quantitatively
observe
apoptosis
cycle
progression.
sulfate
(ZnSO4)
cells
evaluated
by
detecting
markers,
Wnt/β-catenin
signaling
pathway
activity,
oxidative
stress
biomarkers,
regulatory
effect
overexpression.
Results:
Our
revealed
upregulation
samples
compared
normal
samples,
high
expression
linked
poor
prognosis.
repressed
proliferation,
invasion,
migration
cells,
induced
apoptosis,
well
arrested
cycle.
Additionally,
ZnSO4
cytotoxicity
while
overexpression
rescued
ZnSO4-induced,
migration,
proliferation.
Moreover,
had
been
shown
bolster
trigger
pathway,
effects
which
mitigated
Conclusion:
results
implied
that
essential
progression
modulating
homeostasis
cellular
processes.
Targeting
or
may
represent
potential
therapeutic
approach
treatment.
Journal of Orthopaedic Surgery and Research,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: Aug. 17, 2024
Increasing
evidence
shows
the
pivotal
significance
of
miRNAs
in
pathogenesis
osteoporosis.
miR-381-3p
has
been
identified
as
an
inhibitor
osteogenesis.
This
study
explored
role
and
mechanism
postmenopausal
osteoporosis
(PMOP),
most
common
type
Bilateral
ovariectomy
(OVX)
rat
model
was
established
antagomir
administrated
through
tail
vein
vivo.
The
pathological
changes
rats
were
assessed
evaluation
serum
bone
turnover
markers
(BALP,
PINP,
CTX-1),
hematoxylin
eosin
(H&E)
staining,
well
expression
osteoblast
differentiation
biomarkers.
Moreover,
isolated
marrow
mesenchymal
stem
cells
from
OVX-induced
(OVX-BMMSCs)
utilized
to
explore
impact
on
differentiation.
In
addition,
target
gene
downstream
pathway
further
investigated
both
vivo
vitro.
elevated,
whereas
KLF5
suppressed
OVX
rats.
decreased
levels
markers,
improved
trabecular
separation,
promoted
biomarker
ALP
activity
mineralization
suppressed,
biomarkers
impeded
after
overexpression
during
OVX-BMMSCs.
While
contrasting
results
found
inhibition
miR-381-3p.
targets
KLF5,
negatively
affecting
its
Wnt/β-catenin
pathway,
Silencing
restored
activation
induced
by
antagomir.
aggravates
PMOP
inhibiting
osteogenic
targeting
KLF5/Wnt/β-catenin
pathway.
appears
be
a
promising
candidate
for
therapeutic
intervention
PMOP.
PLoS ONE,
Journal Year:
2024,
Volume and Issue:
19(9), P. e0311212 - e0311212
Published: Sept. 30, 2024
Colorectal
cancer
(CRC)
has
become
a
significant
global
health
concern
and
ranks
among
the
leading
causes
of
morbidity
mortality
worldwide.
Due
to
its
malignant
nature,
current
immunotherapeutic
treatments
are
used
tackle
this
issue.
However,
not
all
patients
respond
positively
treatment,
thereby
limiting
clinical
effectiveness
requiring
identification
novel
therapeutic
targets
optimise
strategies.
The
putative
ligand
Siglec-15,
Sialyl-Tn
(STn),
is
associated
with
tumour
progression
synthesised
by
sialyltransferases
ST6GALNAC1
ST6GALNAC2.
deregulation
both
within
literature
remain
limited,
involvement
microRNAs
(miRNAs)
in
STn
production
require
further
elucidation.
Here,
we
identified
miRNAs
involved
regulation
via
computational
approach
analysis
miRNA
binding
sites
were
determined.
In
silico
tools
predicted
miR-21,
miR-30e
miR-26b
regulate
gene,
which
had
shown
upregulated
expression
cohort.
Moreover,
each
displayed
high
affinity
towards
seed
region
.
Additionally,
enrichment
outlined
pathways
several
hallmarks,
including
epithelial
mesenchymal
transition
(EMT)
MYC
progression.
Furthermore,
our
findings
demonstrated
that
profile
was
significantly
downregulated
CRC
tumours,
low
correlated
poor
survival
outcomes
when
compared
patient
data.
comparison
counterpart,
there
no
differences
ST6GALNAC2
between
normal
tissues,
evidenced
immunohistochemistry
analysis.
Immunohistochemistry
staining
highlighted
higher
more
prevalent
human
tissues
regard
conclusion,
integrated
reliant
on
deregulated
sialyltransferase
CRC,
regulated
oncomirs.
We
proposed
other
antigen
Siglec-15/Sia
axis.
Frontiers in Bioscience-Landmark,
Journal Year:
2024,
Volume and Issue:
29(10)
Published: Oct. 12, 2024
Background:
Gastric
cancer
(GC)
is
a
significant
global
health
burden
with
limited
treatment
options.
The
purpose
of
this
study
was
to
investigate
the
role
SLC30A2,
zinc
transporter,
in
GC
development
and
its
capacity
as
target
for
therapy.
Methods:
A
comprehensive
analysis
datasets
(GSE54129
stomach
adenocarcinoma
(STAD)
from
Cancer
Genome
Atlas
(TCGA))
conducted
using
bioinformatics
tools
examine
differential
gene
expression,
focusing
on
SLC30A2.
Functional
assays,
including
Cell
counting
kit-8
(CCK-8)
transwell
were
carried
out
cell
lines
determine
impact
SLC30A2
knockdown
behavior.
Flow
cytometry
utilized
quantitatively
observe
apoptosis
cycle
progression.
sulfate
(ZnSO4)
cells
evaluated
by
detecting
markers,
Wnt/β-catenin
signaling
pathway
activity,
oxidative
stress
biomarkers,
regulatory
effect
overexpression.
Results:
Our
revealed
upregulation
samples
compared
normal
samples,
high
expression
linked
poor
prognosis.
repressed
proliferation,
invasion,
migration
cells,
induced
apoptosis,
well
arrested
cycle.
Additionally,
ZnSO4
cytotoxicity
while
overexpression
rescued
ZnSO4-induced,
migration,
proliferation.
Moreover,
had
been
shown
bolster
trigger
pathway,
effects
which
mitigated
Conclusion:
results
implied
that
essential
progression
modulating
homeostasis
cellular
processes.
Targeting
or
may
represent
potential
therapeutic
approach
treatment.
