Deciphering the Mechanism of Usnic Acid in Type 2 Diabetes Treatment: A Network Pharmacology and Molecular Docking Approach DOI Creative Commons
Miah Roney, Mohd Fadhlizil Fasihi Mohd Aluwi

Published: April 1, 2025

Language: Английский

B. glomerulata Promotes Neuroprotection Against Ischemic Stroke by Inhibiting Apoptosis through the Activation of PI3K/AKT/mTOR Pathway DOI Creative Commons
Zihan Xu, Li Yang,

Peng-lai Pi

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 132, P. 155817 - 155817

Published: June 12, 2024

Brassaiopsis glomerulata (Blum) Regel (B.glomerulata) is recognized as a traditional Chinese medicine (TCM) primarily used for promoting blood circulation and removing stasis. It frequently utilized in the treatment of injuries resulting from falls bumps.

Language: Английский

Citations

10
The Many Facets of PPAR-γ Agonism in Obesity and Associated Comorbidities: Benefits, Risks, Challenges, and Future Directions DOI
Dimitris Kounatidis, Natalia G. Vallianou, Eleni Rebelos

et al.

Current Obesity Reports, Journal Year: 2025, Volume and Issue: 14(1)

Published: Feb. 12, 2025

Language: Английский

Citations

2
Enantioselectivity Enhancement of a Geobacillus thermoleovorans CCR11 Lipase by Rational Design DOI Open Access

Aaron-Salvador Bustos-Baena,

Rodolfo Quintana‐Castro, María Guadalupe Sánchez‐Otero

et al.

Catalysts, Journal Year: 2025, Volume and Issue: 15(2), P. 168 - 168

Published: Feb. 12, 2025

Lipases are enzymes that catalyze the hydrolysis of carboxylic esters at a lipid–water interface and able to reactions such as alcoholysis, esterification, transesterification, enantioselective synthesis in organic media. They important biocatalysts for biotechnological industrial applications—such food flavor industry—and production biopharmaceuticals, biofuels, biopolymers, detergents. A desirable property lipases is stereoselectivity chemicals with high optical purity. In this work, we report improvement capabilities Geobacillus thermoleovorans CCR11 lipase. By means rational design bioinformatic approaches, six amino acids catalytic cavity lipase LipTioCCR11 were substituted resulting an increase optimum temperature enzyme resistance presence solvents hydrolytic reactions, promotion recognition R isomers importance pharmaceutical industries.

Language: Английский

Citations

2
Combining Network Pharmacology, Molecular Docking and Experimental Validation to Explore the Effects and Mechanisms of Indirubin on Acute Lymphoblastic Leukemia DOI Creative Commons
Lu Jin, Yinghui Guan, Xue Li

et al.

Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 1083 - 1103

Published: Feb. 1, 2025

To investigate the effects and underlying mechanisms of indirubin in treating ALL using network pharmacology experimental validation. Potential targets indirubin- ALL-related genes were identified public databases. Core filtered through protein-protein interaction analysis Cytoscape. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses conducted to explore potential against ALL. Drug-disease-functional annotation-signaling maps constructed. Molecular docking between core proteins was performed AutoDock Vina software. Finally, both vitro vivo experiments validate these findings. PPI eight ALL: AKT1, CASP3, mammalian target rapamycin. GO KEGG suggested that mechanism action involves multiple biological functions signaling pathways, with PI3K-AKT likely playing a central role. findings further confirmed strong binding affinity for targets. Both demonstrated inhibited cell proliferation induced cycle arrest apoptosis; may involve pathway. The pharmacology, as well validation elucidated, offering new insights therapeutic avenues treatment

Language: Английский

Citations

2
The Mechanism of Bisphenol S‐Induced Atherosclerosis Elucidated Based on Network Toxicology, Molecular Docking, and Machine Learning DOI Open Access
Bing Guo, He Xuan

Journal of Applied Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

The increasing prevalence of environmental pollutants has raised public concern about their potential role in diseases such as atherosclerosis (AS). Existing studies suggest that chemicals, including bisphenol S (BPS), may adversely affect cardiovascular health, but the specific mechanisms remain unclear. This study aims to elucidate effects BPS on AS and underlying mechanisms. Through an extensive search databases ChEMBL, STITCH, SwissTargetPrediction, SuperPred, SEA, GEO, we identified 34 targets related BPS-induced AS. A target network was constructed using STRING platform Cytoscape software. GO KEGG functional enrichment analysis DAVID database revealed promote occurrence by interfering with critical biological processes glutathione metabolism, nitrogen tyrosine metabolism. followed selection 4 core targets-aminopeptidase n (ANPEP), alcohol dehydrogenase 5 (ADH5), lysosomal pro-x carboxypeptidase (PRCP), microsomal s-transferase 1 (MGST1)-using five machine learning methods. These play a pivotal Furthermore, molecular docking confirmed tight binding between these targets. In conclusion, this provides theoretical framework for understanding contributes scientific evidence development prevention treatment strategies triggered exposure.

Language: Английский

Citations

2
Glucose-modified BSA/procyanidin C1 NPs penetrate the blood-brain barrier and alleviate neuroinflammation in Alzheimer's disease models DOI

Linyan Duan,

Zhizhong Hao,

Rong Ji

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 268, P. 131739 - 131739

Published: April 22, 2024

Language: Английский

Citations

7
Narciclasine attenuates sepsis-associated acute kidney injury through the ESR1/S100A11 axis DOI
Liping Yin, Xiaofei Huang, Beibei Zhang

et al.

Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 14, 2025

Language: Английский

Citations

1
Anti-osteoporosis effect of Mori Follum- Portulaca oleracea L.- Lycium barbarum L. extract based on network pharmacology and experimental validation and its utilization in functional yogurt DOI
Mingjie Jia, Xue Yang, Yihong Bao

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 59, P. 103974 - 103974

Published: March 30, 2024

Language: Английский

Citations

5
Green Tea Polyphenol (-)-Epicatechin Pretreatment Mitigates Hepatic Steatosis in an In Vitro MASLD Model DOI Creative Commons
Marija Hefer, Ana G. Petrovic, Lucija Kuna

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(8), P. 8981 - 8994

Published: Aug. 16, 2024

Abstract: Metabolic dysfunction-associated steatotic liver disease (MASLD), previously known as non-alcoholic fatty (NAFLD), is becoming more prominent globally due to an increase in the prevalence of obesity, dyslipidemia, and type 2 diabetes. A great deal studies have proposed potential treatments for MASLD, with few them demonstrating promising results. The aim this study was investigate effects (-)-epicatechin (EPI) on development MASLD vitro model using HepG2 cell line by determining metabolic viability cells levels PPARα, PPARγ, GSH. were pretreated 10, 30, 50, 100 μM EPI 4 h assess lipid metabolism. culture established hepatocytes which exposed 1.5 mM oleic acid (OA) 24 h. Moreover, colorimetric MTS assay used order determine cells, PPARα PPARγ protein determined enzyme-linked immunosorbent (ELISA), accumulation visualized Oil Red O Staining method. Also, intracellular glutathione (GSH) measured level oxidative stress. shown treated OA. after incubation OA, significantly decreased, a 71%, compared EPI, where 74–86% respect concentration experiment. Furthermore, GSH exhibited decrease response increasing concentrations. Pretreatment has demonstrated effect vitro. Therefore, considering that mediates metabolism it should be considered hepatoprotective agent future research.

Language: Английский

Citations

5
Role of Gut Microbiota, Immune Imbalance, and Allostatic Load in the Occurrence and Development of Diabetic Kidney Disease DOI Creative Commons

Yi Han,

Huijuan Zheng, Bo Du

et al.

Journal of Diabetes Research, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 17

Published: Dec. 6, 2023

Diabetic kidney disease (DKD) is a prevailing complication arising from diabetes mellitus. Unfortunately, there are no trustworthy and efficacious treatment modalities currently available. In recent times, compelling evidence has emerged regarding the intricate correlation between gut microbiota, which considered largest immune organ within human physique. Various investigations have demonstrated that perturbation of microbiota its associated metabolites potentially underlie etiology progression DKD. This phenomenon may transpire through both innate adaptive immunity, leading to burdensome allostatic load on body ultimately culminating in development Within this literature review, we aim delve into interplay metabolites, system context Furthermore, strive explore elucidate potential chemical interventions could hold promise for DKD, thereby offering invaluable insights directions future research endeavors.

Language: Английский

Citations

11