FAP expression dynamics and role in silicosis: Insights from epidemiological and experimental models

Environmental Pollution, Journal Year: 2024, Volume and Issue: 356, P. 124311 - 124311

Published: June 3, 2024

Language: Английский

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Fibroblast activation protein is a cellular marker of fibrotic activity in canine idiopathic pulmonary fibrosis

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: July 31, 2024

Canine idiopathic pulmonary fibrosis (CIPF) is a progressive fibrotic interstitial lung disease of unknown etiology, afflicting aging West Highland white terriers (WHWTs) and leading to respiratory failure. Fibroblast activation protein (FAP), protease overexpressed in many cancers, upregulated humans. The aim this study was investigate FAP as marker active biopsies from WHWTs affected with CIPF, well the potential plasmatic biomarker. After establishing scoring system evaluate severity activity on histopathological sections, anti-FAP immunohistochemistry performed healthy CIPF samples. expression characterized using both visual digital quantitative pathology software analyses then correlated activity. Levels were measured by enzyme-linked immunosorbent assay compared dogs. Lung samples 22 collected. According system, they classified cases mild (5), moderate (9) severe (8) attributed scores Fifteen non-fibrotic. Healthy FAP-negative, whereas fibroblasts FAP-positive 20 immunohistochemical mildly ( p < 0.05; R 2 = 0.22) but highly 0.001; 0.68). Digital image analysis detected higher percentage cells areas 0.001) distributed outside mature lesions, clustered or scattered within alveolar septa. On other hand, significantly lower dogs 0.01). In conclusion, provides valuable histological assess CIPF. It demonstrates that good cellular thus constitutes promising target be exploited for diagnostic therapeutic applications. Additionally, it suggests FAP, although non-specific, could altered

Language: Английский

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Cardiac fibroblast activation: The lower the better

International Journal of Cardiology, Journal Year: 2024, Volume and Issue: 409, P. 132162 - 132162

Published: May 14, 2024

Language: Английский

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Enhanced Purification of Uterine Smooth Muscle Cells from Adenomyosis Using a Novel Dual- Enzyme Digestion Method

New Biotechnology, Journal Year: 2024, Volume and Issue: 84, P. 151 - 161

Published: Nov. 7, 2024

Uterine adenomyosis causing attention to the abnormal smooth muscle layer has recently received increasing attention, which created need for a method that can efficiently collect high purity uterine cells (USMC) in laboratory setting. In this study, we explored composition ratios of digestion solution obtain optimal (DM4). Furthermore, tested superiority two methods obtaining adenomyotic by comparing DM4 with conventional tissue adhesion growth rate, single-cell RNA sequencing, and fluorescence identification. The results demonstrated USMCs produced exhibited significantly higher rates proliferation were more effective generating mature compared those obtained using adherence methods, is inclined isolate progenitor cell population. This study presents reliable isolating provides solid foundation future studies on etiology mechanism adenomyosis.

Language: Английский

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Analysis of cellular NO-GC expression in the murine heart and lineage determination in angiotensin II-induced fibrosis

iScience, Journal Year: 2024, Volume and Issue: 28(1), P. 111615 - 111615

Published: Dec. 16, 2024

Language: Английский

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