Astragalus Membranaceus: Exploring its Protective Role in Heat Stroke via Integrated Transcriptomic and Molecular Docking Approaches DOI Creative Commons

Defeng Yin,

Yao Yang, Qin Guo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Abstract Objective: This study aims to explore the therapeutic potential of Astrolus Membranaceus in treating heatstroke and its targets. Method: was conducted at Affiliated Hospital Southwest Medical University, peripheral blood samples were collected from 10 patients (HS=10) healthy individuals (NC=10) for RNA sequencing. Perform differential analysis using DESeq2 software package. In addition, active ingredients targets Astragalus membranaceus screened TCMSP database. Intersection target obtain cross target. Then perform GO KEGG on intersection targets. Build a protein interaction network with STRING website Cytoscape software, molecular docking between core molecules AutoDock Tools. Results: RNA sequencing results showed that compared NC group, HS group had total 2042 differentially expressed RNAs. After taking RNA, 23 intersecting obtained. found enrichment cellular components genes is mainly extracellular region. The functional involves function regulators. biological processes related factors include regulation function, multicellular organic processes, response stress. enriched pathways crossover MAPK signaling pathway. key TP53, BCL2, MMP9 protein-protein identified cytoHubba. indicate quercetin forms hydrogen bonds MMP9, low binding energies. Conclusion: reveals mechanism heatstroke, providing scientific basis further drug development clinical application.

Language: Английский

Metabolomics combined with network pharmacology reveals the protective effect of astragaloside IV on alcoholic liver disease DOI
Jinfang Hao,

Ruixian Hu,

Jianming Zhao

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: unknown, P. 156032 - 156032

Published: Sept. 1, 2024

Language: Английский

Citations

2
BuyangHuanwu Decoction alleviates Endothelial Cell Apoptosis and Coronary Microvascular Dysfunction via Regulation of the MAPKK4/p38 Signaling Axis DOI Creative Commons

Xing Chang,

Dan Wu, Xin Gao

et al.

International Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 21(13), P. 2464 - 2479

Published: Jan. 1, 2024

MAPKK4 has been implicated in the pathological mechanisms underlying myocardial and vascular injury, specifically influencing endothelial cell damage programmed death via subcellular pathways. Nevertheless, regulatory role of coronary microvascular injury following infarction remains unconfirmed, exploration targeted mitochondrial protective therapeutic agents unaddressed. In light this gap, we established a gene-modified mouse model ischemia-reperfusion employed Buyang Huanwu decoction (BYHW), traditional cardiovascular formula, to assess its efficacy treating post-ischemia-reperfusion. The study aimed elucidate mechanism by which BYHW mitigates induced through attenuation apoptosis. Experimental outcomes revealed that high-dose significantly ameliorated post-ischemia-reperfusion, restoring structural integrity microvasculature reducing inflammation oxidative stress. Contrarily, transgenic mice overexpressing MAPKK4, intervention failed attenuate To further investigate, simulated hypoxia/reoxygenation cells using MAPKK4-related cellular gene modification model. results indicated attenuates inflammatory enhances viability hypoxic stress, inhibiting apoptosis pathway. However, overexpression MAPKK4/p38 negated effects BYHW, showing no impact on stress under conditions. Molecular interaction studies confirmed active components Astragaloside IV Ligustrazine, interact with MAPKK4/P38 axis.

Language: Английский

Citations

1
Exploring the active ingredients and mechanisms of Liujunzi decoction in treating hepatitis B: a study based on network pharmacology, molecular docking, and molecular dynamics simulations DOI

Qing Ma,

Wenjun Li,

Wenying Wu

et al.

Computer Methods in Biomechanics & Biomedical Engineering, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: Nov. 13, 2024

Liujunzi decoction (LJZD) is commonly used to treat hepatitis B virus (HBV), though its active ingredients and mechanisms are not fully known. This study identified core targets components of LJZD for treating (HB) through network pharmacology, molecular docking, dynamics simulation. Screening from databases yielded 533 components, 2619 LJZD, 2910 HB, with 891 intersecting targets. STRING CytoHubba analyses AR VDR as targets, key pathways including PI3K-Akt MAPK. The findings clarify LJZD's multicomponent, multitarget mechanisms, supporting clinical application HB treatment.

Language: Английский

Citations

1
Network Pharmacology to Reveal the Mechanism of Fufang Banmao Capsule for Treating Unresectable Primary Liver Cancer and Clinical Data Validation DOI
Youwen Hu, Yangyang Xiao, Lijun Wan

et al.

Current Pharmaceutical Design, Journal Year: 2024, Volume and Issue: 30(35), P. 2785 - 2796

Published: Aug. 2, 2024

Fufang Banmao capsule (FFBM), a traditional Chinese medicine, has been used to treat primary liver cancer (PLC) for several years. However, the bioactive ingredients, and mechanism of FFBM treating PLC remains unclear. Our objective is utilize network pharmacology investigate these aspects subsequently validate their effectiveness through clinical data.

Language: Английский

Citations

0
Astragalus Membranaceus: Exploring its Protective Role in Heat Stroke via Integrated Transcriptomic and Molecular Docking Approaches DOI Creative Commons

Defeng Yin,

Yao Yang, Qin Guo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Abstract Objective: This study aims to explore the therapeutic potential of Astrolus Membranaceus in treating heatstroke and its targets. Method: was conducted at Affiliated Hospital Southwest Medical University, peripheral blood samples were collected from 10 patients (HS=10) healthy individuals (NC=10) for RNA sequencing. Perform differential analysis using DESeq2 software package. In addition, active ingredients targets Astragalus membranaceus screened TCMSP database. Intersection target obtain cross target. Then perform GO KEGG on intersection targets. Build a protein interaction network with STRING website Cytoscape software, molecular docking between core molecules AutoDock Tools. Results: RNA sequencing results showed that compared NC group, HS group had total 2042 differentially expressed RNAs. After taking RNA, 23 intersecting obtained. found enrichment cellular components genes is mainly extracellular region. The functional involves function regulators. biological processes related factors include regulation function, multicellular organic processes, response stress. enriched pathways crossover MAPK signaling pathway. key TP53, BCL2, MMP9 protein-protein identified cytoHubba. indicate quercetin forms hydrogen bonds MMP9, low binding energies. Conclusion: reveals mechanism heatstroke, providing scientific basis further drug development clinical application.

Language: Английский

Citations

0