TEFM facilitates uterine corpus endometrial carcinoma progression by activating ROS-NFκB pathway DOI Creative Commons

Jia Lei,

Qingguo Zhu,

Jianghao Guo

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 27, 2024

Mitochondrial transcription elongation factor (TEFM) is a recently discovered involved in mitochondrial DNA replication and transcription. Previous studies have reported that abnormal TEFM expression can disrupt the assembly of respiratory chain thus function. However, role on Uterine corpus endometrial carcinoma (UCEC) progression remains unclear. The present study aims to investigate tumor tissue UCEC effect malignant phenotype cells. expressions were measured tissues cell lines by immunohistochemistry, Western blotting, real-time quantitative PCR assays. Besides, effects knockdown or overexpression growth, metastasis, apoptosis, autophagy also determined using EdU, colony formation, flow cytometry, TUNEL, transmission electron microscopy Xenograft model was used confirm proliferative potential UECE cells vivo. Our bioinformatics analysis CPTAC data showed abnormally overexpressed its upregulation significantly associated with poor survival patients UCEC. We found promoted growth metastasis Mechanically, impaired function mitochondria, decreased their membrane activated AKT-NFκB pathway promoting reactive oxygen species (ROS) production, leading enhanced intracellular metastasis. This demonstrates positively regulates promote cells, which provides prognostic biomarker therapeutic target for treatment

Language: Английский

TEFM facilitates uterine corpus endometrial carcinoma progression by activating ROS-NFκB pathway DOI Creative Commons

Jia Lei,

Qingguo Zhu,

Jianghao Guo

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 27, 2024

Mitochondrial transcription elongation factor (TEFM) is a recently discovered involved in mitochondrial DNA replication and transcription. Previous studies have reported that abnormal TEFM expression can disrupt the assembly of respiratory chain thus function. However, role on Uterine corpus endometrial carcinoma (UCEC) progression remains unclear. The present study aims to investigate tumor tissue UCEC effect malignant phenotype cells. expressions were measured tissues cell lines by immunohistochemistry, Western blotting, real-time quantitative PCR assays. Besides, effects knockdown or overexpression growth, metastasis, apoptosis, autophagy also determined using EdU, colony formation, flow cytometry, TUNEL, transmission electron microscopy Xenograft model was used confirm proliferative potential UECE cells vivo. Our bioinformatics analysis CPTAC data showed abnormally overexpressed its upregulation significantly associated with poor survival patients UCEC. We found promoted growth metastasis Mechanically, impaired function mitochondria, decreased their membrane activated AKT-NFκB pathway promoting reactive oxygen species (ROS) production, leading enhanced intracellular metastasis. This demonstrates positively regulates promote cells, which provides prognostic biomarker therapeutic target for treatment

Language: Английский

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