A Stemness‐Enhanced Eye Drop Stimulates Corneal Regeneration via Sustained Release of Versican and Providing Lubricated Substrate DOI Open Access

Shiding Li,

Siyi Zhang, Fei Fang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract Stem cells are vital for tissue regeneration, and their dysfunction can lead to diseases like aplastic anemia Alzheimer's disease. Limbal epithelial stem (LESCs) maintain corneal transparency as the sole source of epithelium. Dysfunction or deficiency LESCs causes opacity, neovascularization blindness, known limbal cell (LSCD). Current LSCD treatment mainly relies on transplantation, which faces challenges limited sources immune rejection, making therapy challenging often ineffective. This study introduces a cell‐free, stemness‐enhanced eye drop (SEED @ ) stimulate autologous regeneration. SEED , thermosensitive Pluronic F‐127 hydrogel loaded with versican, promote proliferation, chemotaxis, migration while maintaining stemness via Wnt/β‐catenin signaling pathway. In vivo, alone lubricate cornea repair in mouse alkali burn model. addition, forms thin, transparent layer ocular surface, enabling sustained versican release up 24 h. Notably, twice‐daily application significantly accelerated regeneration rabbit model, achieving approximately 95% by day 14. Collectively, shows great potential treating offers promising drug delivery platform diseases.

Language: Английский

Advancements in Hydrogels for Corneal Healing and Tissue Engineering DOI Creative Commons
Kevin Y. Wu,

S.Y. Qian,

Anne Faucher

et al.

Gels, Journal Year: 2024, Volume and Issue: 10(10), P. 662 - 662

Published: Oct. 16, 2024

Hydrogels have garnered significant attention for their versatile applications across various fields, including biomedical engineering. This review delves into the fundamentals of hydrogels, exploring definition, properties, and classification. Hydrogels, as three-dimensional networks crosslinked polymers, possess tunable properties such biocompatibility, mechanical strength, hydrophilicity, making them ideal medical applications. Uniquely, this article offers original insights application hydrogels specifically corneal tissue engineering, bridging a gap in current research. The further examines anatomical functional complexities cornea, highlighting challenges associated with pathologies reliance on donor corneas transplantation. Considering global shortage corneas, discusses potential hydrogel-based materials Emphasis is placed synthesis processes, physical chemical crosslinking, integration bioactive molecules. Stimuli-responsive which react to environmental triggers, are identified promising tools drug delivery repair. Additionally, clinical explored, showcasing efficacy trials. Finally, addresses regulatory approval need research fully realize offering outlook future developments field.

Language: Английский

Citations

5
The Spatial Transcriptomic Atlas of Human Limbus and Vital Niche Microenvironment Regulating the Fate of Limbal Epithelial Stem Cells DOI Creative Commons

Shiding Li,

Hao Sun,

Fei Fang

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(3), P. 52 - 52

Published: March 25, 2025

Purpose: This study aimed to generate the spatial atlas of human limbus using transcriptomic technology and reveal deep interaction among niche microenvironment. Methods: The was performed 10× Genomics Space Ranger software platform. Single-cell RNA sequencing data limbal epithelial stem cells (LESCs) were downloaded for integrating analysis. Results: We profiled more than 400 spots within each sample spatially located major cell types area. LESCs localized mainly in basement membrane, situated predominantly stromal Next, divided into four regions based on histological structure, differential expressed genes analyzed. Notably, GPHB5 highly epithelium middle region co-staining with deltaNp63 suggested it might be a novel potential biomarker LESCs. Subsequently, mesenchymal found exhibit greatest amounts ligands associated widespread activity COL6A2/CD44 signaling cells, melanocytes, immune indicate its essential role mediating bidirectional communication via collagen pathway. Conclusions: research mapped positioning key detailed interactions between types. These findings provide foundation further LESC enhance our understanding corneal biology.

Language: Английский

Citations

0
Advances in Regenerative Medicine, Cell Therapy, and 3D Bioprinting for Corneal, Oculoplastic, and Orbital Surgery DOI
Kevin Y. Wu, Natalie Kearn,

Doanh Truong

et al.

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Limbal Explant Cultures on Amniotic Membrane: The Effects of Passaging the Explants on Cell Phenotype DOI
Mehmet Gürdal, Kemal Baysal,

İsmet Durak

et al.

Experimental Eye Research, Journal Year: 2025, Volume and Issue: 255, P. 110392 - 110392

Published: April 16, 2025

Language: Английский

Citations

0
Delivery of Itgb1-siRNA by triptolide-modified and anti-Flt1 peptide-guided ionizable cationic LNPs for targeted therapy of corneal neovascularization DOI
Yuwen Song, Tingting Xu, Hao Li

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 383, P. 113811 - 113811

Published: May 3, 2025

Language: Английский

Citations

0
MicroRNAs in Corneal Diseases: Emerging Roles as Biomarkers, Regulators, and Therapeutics DOI
Liangbo Chen,

Shiding Li,

Yao Fu

et al.

The Ocular Surface, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
A Stemness‐Enhanced Eye Drop Stimulates Corneal Regeneration via Sustained Release of Versican and Providing Lubricated Substrate DOI Open Access

Shiding Li,

Siyi Zhang, Fei Fang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract Stem cells are vital for tissue regeneration, and their dysfunction can lead to diseases like aplastic anemia Alzheimer's disease. Limbal epithelial stem (LESCs) maintain corneal transparency as the sole source of epithelium. Dysfunction or deficiency LESCs causes opacity, neovascularization blindness, known limbal cell (LSCD). Current LSCD treatment mainly relies on transplantation, which faces challenges limited sources immune rejection, making therapy challenging often ineffective. This study introduces a cell‐free, stemness‐enhanced eye drop (SEED @ ) stimulate autologous regeneration. SEED , thermosensitive Pluronic F‐127 hydrogel loaded with versican, promote proliferation, chemotaxis, migration while maintaining stemness via Wnt/β‐catenin signaling pathway. In vivo, alone lubricate cornea repair in mouse alkali burn model. addition, forms thin, transparent layer ocular surface, enabling sustained versican release up 24 h. Notably, twice‐daily application significantly accelerated regeneration rabbit model, achieving approximately 95% by day 14. Collectively, shows great potential treating offers promising drug delivery platform diseases.

Language: Английский

Citations

1