Effects of 3D-printed exosome-functionalized brain acellular matrix hydrogel on neuroinflammation in rats following cerebral hemorrhage DOI Creative Commons
Aobo Zhang,

Boyu Sun,

Chengrui Nan

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 20, 2025

Exosome-based therapeutics have garnered significant attention for intracerebral hemorrhage (ICH) treatment due to their capacity regulate metabolic dysregulation, restore cellular homeostasis, and modulate the injury microenvironment via bioactive cargoes such as microRNAs proteins. However, rapid systemic clearance enzymatic degradation critically limit therapeutic efficacy. To address this challenge, we engineered a three-dimensional (3D) bioprinted scaffold capable of encapsulating sustaining release human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-exos). Based on previous research [1-3], was composed decellularized brain matrix (dECM), gelatin-methacryloyl (GelMA), silk fibroin (SF) crosslinked with photoinitiator. hUCMSC-exos were precisely incorporated extrusion-based 3D bioprinting. Release kinetics assessed in vitro elution vivo imaging. An ICH rat model received stereotaxic implantation exosome-laden (dECM@exo). Neuroinflammatory markers (IL-6, TNF-α, IL-10) apoptotic activity (JC-1, Annexin V/PI, TUNEL) quantified. Neurological outcomes longitudinally evaluated using modified Longa scale, Bederson scoring, sensorimotor tests (rotarod, forelimb placement) at 1, 4, 7 14 days post-ICH. dECM@exo demonstrated sustained exosome over days, significantly promoting neural tissue regeneration while attenuating perihematomal edema. Mechanistically, modulated pathological MMP inflammatory cytokine expression, thereby restoring extracellular homeostasis reducing neuronal apoptosis. The findings demonstrate that biological effectively maintains microenvironmental early stages improves associated condition. is poised serve robust platform drug delivery biotherapy treatment, offering viable alternative managing

Language: Английский

Effects of 3D-printed exosome-functionalized brain acellular matrix hydrogel on neuroinflammation in rats following cerebral hemorrhage DOI Creative Commons
Aobo Zhang,

Boyu Sun,

Chengrui Nan

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 20, 2025

Exosome-based therapeutics have garnered significant attention for intracerebral hemorrhage (ICH) treatment due to their capacity regulate metabolic dysregulation, restore cellular homeostasis, and modulate the injury microenvironment via bioactive cargoes such as microRNAs proteins. However, rapid systemic clearance enzymatic degradation critically limit therapeutic efficacy. To address this challenge, we engineered a three-dimensional (3D) bioprinted scaffold capable of encapsulating sustaining release human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-exos). Based on previous research [1-3], was composed decellularized brain matrix (dECM), gelatin-methacryloyl (GelMA), silk fibroin (SF) crosslinked with photoinitiator. hUCMSC-exos were precisely incorporated extrusion-based 3D bioprinting. Release kinetics assessed in vitro elution vivo imaging. An ICH rat model received stereotaxic implantation exosome-laden (dECM@exo). Neuroinflammatory markers (IL-6, TNF-α, IL-10) apoptotic activity (JC-1, Annexin V/PI, TUNEL) quantified. Neurological outcomes longitudinally evaluated using modified Longa scale, Bederson scoring, sensorimotor tests (rotarod, forelimb placement) at 1, 4, 7 14 days post-ICH. dECM@exo demonstrated sustained exosome over days, significantly promoting neural tissue regeneration while attenuating perihematomal edema. Mechanistically, modulated pathological MMP inflammatory cytokine expression, thereby restoring extracellular homeostasis reducing neuronal apoptosis. The findings demonstrate that biological effectively maintains microenvironmental early stages improves associated condition. is poised serve robust platform drug delivery biotherapy treatment, offering viable alternative managing

Language: Английский

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