Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract Mitochondrial transfer emerges as a promising therapy for the restoration of mitochondrial function in damaged cells, mainly due to its limited immunogenicity. However, isolated mitochondria rapidly lose because they produce little energy outside cells. Therefore, this study investigates whether near infrared (NIR)‐mediated nicotinamide adenine dinucleotide (NADH) pre‐treatment enhances and stability mitochondria‐donor cells prior transplantation. Clinical applications NADH, an essential electron donor oxidative phosphorylation process, are restricted cellular uptake NADH. To address this, photo‐mediated method optimizes direct NADH delivery into increases absorption. L6 treated with irradiated NIR enhanced uptake, significantly improving production function. Importantly, improved functional characteristics maintained even after isolation from Primed mitochondria, i.e., those by NIR‐mediated (P‐MT), encapsulated fusogenic liposomes delivered muscle dysfunction. Compared conventional P‐MT promote greater recovery regeneration. These findings suggest that is effective strategy function, has potential lead novel treatments disorders degeneration.

Language: Английский

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Mendelian randomization analysis to identify potential drug targets for osteoarthritis

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0316824 - e0316824

Published: Feb. 11, 2025

Background Osteoarthritis (OA) is a prevalent chronic joint disease for which there lack of effective treatments. In this study, we used Mendelian randomization analysis to identify circulating proteins that are causally associated with OA-related traits, providing important insights into potential drug targets OA. Method Causal associations between 1553 and five traits were assessed in large-scale two-sample MR analyses using Wald ratio or inverse variance weighting, the results corrected Bonferroni. addition, sensitivity performed validate reliability results, including reverse Steiger filtering ensure causal direction OA; Bayesian co-localization phenotypic scanning eliminate confounding effects horizontal pleiotropy. External validation was exclude incidental findings novel plasma protein quantitative trait loci. Finally, online tool Enrichr utilized screen drugs molecular docking predict binding modes energies most stable likely drugs. Result Four ultimately found be reliably three features: DNAJB12 USP8 knee OA, IL12B spinal RGMB thumb The ORs above 1.51 (95% CI, 1.26–1.81), 1.72 1.42–2.08), 0.87 0.81–0.92), 0.59 0.47–0.75), respectively. Drug-predicting small molecules (doxazosin, XEN 103, montelukast) simultaneously target proteins, DNAJB12, USP8, IL12B, docked well. Conclusion Based on our comprehensive analysis, can draw conclusion relationship genetic levels , risk respective OA.They may options OA screening prevention clinical practice. They also serve as candidate future mechanism exploration selection.

Language: Английский

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Revealing the Landscape Crosstalk Between Reproductive System and Organs Aging

The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(8)

Published: April 27, 2025

ABSTRACT The reproductive system is a vital component of the human body. In modern society, due to various socio‐economic reasons, an increasing number couples are choosing postpone childbearing. Research into impact aging on becoming increasingly important. As people age, there decline in across levels, from testes males spermatogonia cells, and ovaries females oocytes. not only affects itself but also has implications for other organs systems Conversely, can damage system. This review organizes changes that occur within as result focuses interactions between systems. Additionally, this summarizes current therapies aimed at delaying aging, which may provide insights future interventions targeting

Language: Английский

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Sarcopenic obesity: prospects of mitochondrial transplantation to combat metabolic deterioration in aging

Acta Materia Medica, Journal Year: 2025, Volume and Issue: 4(3)

Published: Jan. 1, 2025

Aging impairs adipose tissue function and contributes to metabolic disorders, such as obesity insulin resistance. Chronic inflammation is mediated by cytokines, including IL-6, IL-1β, TNF-α, a pivotal factor in aging that links mitochondrial dysfunction activation of the NLRP3 inflammasome aged tissue. Moreover, distinct depots exhibit complex molecular cellular functions related deterioration conventional regulation pathways. Unraveling these mechanisms crucial for developing therapeutic strategies enhance health promote longevity. This review elucidates current understanding lipid metabolism, resistance, senescence, dysfunction. improve quality control tissue, transplantation, hold promise addressing Finally, novel approach transplantation proposed using exosome cargo sustain healthy metabolism thermogenesis populations. These findings lay groundwork innovative therapies preventive mitigate impact aging.

Language: Английский

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